cbc5e17ce713a7076a9f95991e10262079780cc8 max Fri May 16 10:27:58 2025 -0700 overload -c to skip internal markers diff --git src/cbPyLib/cellbrowser/seurat.py src/cbPyLib/cellbrowser/seurat.py index fcc340c..530b9f4 100644 --- src/cbPyLib/cellbrowser/seurat.py +++ src/cbPyLib/cellbrowser/seurat.py @@ -366,31 +366,32 @@ "convert the data and add the dataset under htmlDir") parser.add_option("-p", "--port", dest="port", action="store", type="int", help="only with --htmlDir: start webserver on port to serve htmlDir") parser.add_option("-x", "--skipMatrix", dest="skipMatrix", action="store_true", default = False, help="do not convert the matrix, saves time if the same one has been exported before to the " "same outDir directory") parser.add_option("", "--threads", dest="threadCount", action="store", type="int", default=0, help="activate multiprocess strategy, default thread count is 0, which uses no multithreading") parser.add_option("-m", "--skipMarkers", dest="skipMarkers", action="store_true", default = False, - help="do not calculate cluster-specific markers with FindAllMarkers(), saves a lot of time") + help="do not calculate cluster-specific markers with FindAllMarkers(), saves a lot of time. Also use " + "this option if the dataset includes markers in obj@misc and you do not want to use them.") parser.add_option("-c", "--clusterField", dest="clusterField", action="store", help="Cluster field to color on, by default this is the @ident slot of the Seurat object but it can also be any other meta data field of the @meta.data slot") parser.add_option("", "--markerFile", dest="markerFile", action="store", help="Instead of calculating cluster markers again, use this file. Format: cluster,gene,pVal + any other fields. Or alternatively the native Seurat cluster markers format, as created by write.table") parser.add_option("", "--useMtx", dest="useMtx", action="store_true", help="Write a .mtx.gz file, instead of a tsv.gz file. Necessary for big datasets.") parser.add_option("-s", "--matrixSlot", dest="matrixSlot", action="store", default="counts,data,scale.data", help="Export this slot of the matrix. Can be 'counts', 'scale.data' or 'data'. Default is %default. The first value is the default matrix for coloring the plot.") parser.add_option("", "--assay", dest="assay", action="store", help="Select the Seurat3 assay to export.")