src/hg/encode/DAFs/WoldRnaSeq.daf 1.3
1.3 2009/03/06 00:52:10 mikep
updated DAF to handle more raw data files
Index: src/hg/encode/DAFs/WoldRnaSeq.daf
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/encode/DAFs/WoldRnaSeq.daf,v
retrieving revision 1.2
retrieving revision 1.3
diff -b -B -U 4 -r1.2 -r1.3
--- src/hg/encode/DAFs/WoldRnaSeq.daf 14 Feb 2009 08:11:52 -0000 1.2
+++ src/hg/encode/DAFs/WoldRnaSeq.daf 6 Mar 2009 00:52:10 -0000 1.3
@@ -6,9 +6,9 @@
# Lab and general info
grant Wold
lab Caltech
dataType RnaSeq
-variables cell, localization, rnaExtract, mapAlgorithm
+variables cell, localization, rnaExtract, mapAlgorithm, readType
assembly hg18
dafVersion 0.2.2
# Track/view definition
@@ -29,36 +29,69 @@
type bed 12
hasReplicates yes
required no
-view Alignments
+view Aligns
longLabelPrefix Caltech RNA-Seq Map Algorithm Alignments
type bed 12
hasReplicates yes
required no
-view PairedAligns
+view Paired
longLabelPrefix Caltech RNA-Seq Map Algorithm Paired Alignments
type bed 12
hasReplicates yes
required no
+# For 2x75 datasets, raw Illumina reads (RawData, fasta format, query75.txt files) are run through bowtie 0.9.8.1 with up to 2 mismatches
+# and the resulting mappings are stored (RawData2, bowtie format, hg18sp75spike.bowtie.txt files) for up to ten matches per-read to the genome,
+# spiked controls and UCSC knownGene splice junctions. Reads that were not mapped by bowtie (RawData3, fasta format, unmapped.fa files)
+# are then mapped onto the genome using blat and filtered using pslReps (RawData4, psl format, bowtierej.hg18.blatbetter files).
+# The bowtie and blat mappings are then analyzed by ERANGE3.0.2 to generate wiggles (RawSignal, wiggle format, .graph files),
+# bed files of all reads and splices (Aligns, PairedAligns, bed format, all.bed files),
+# all bowtie and blat splices (Splices, bed format, allsplices.bed files),
+# and blat-only splices (Splices, bed format, blatsplices.bed),
+# as well as RPKM expression level measurements at the gene-level (RawData5, rpkm format, final.rpkm),
+# exon-level (RawData6, rpkm format, gencode_exon.rpkm), and candidate novel exons (RawData7, rpkm format, accepted.rpkm).
+
+# query75.txt
view RawData
type fasta
hasReplicates yes
required no
+# hg18sp75spike.bowtie.txt
view RawData2
-type rpkm
+type bowtie
hasReplicates yes
required no
+# unmapped.fa
view RawData3
-type bowtie
+type fasta
hasReplicates yes
required no
+# bowtierej.hg18.blatbetter
view RawData4
type psl
hasReplicates yes
required no
+# final.rpkm
+view RawData5
+type rpkm
+hasReplicates yes
+required no
+
+# gencode_exon
+view RawData6
+type rpkm
+hasReplicates yes
+required no
+
+# accepted.rpkm
+view RawData7
+type rpkm
+hasReplicates yes
+required no
+