src/hg/encode/DAFs/WoldRnaSeq.daf 1.4
1.4 2009/03/06 16:53:30 mikep
adding rawdata files for Wold pipeline input data
Index: src/hg/encode/DAFs/WoldRnaSeq.daf
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/encode/DAFs/WoldRnaSeq.daf,v
retrieving revision 1.3
retrieving revision 1.4
diff -b -B -U 1000000 -r1.3 -r1.4
--- src/hg/encode/DAFs/WoldRnaSeq.daf 6 Mar 2009 00:52:10 -0000 1.3
+++ src/hg/encode/DAFs/WoldRnaSeq.daf 6 Mar 2009 16:53:30 -0000 1.4
@@ -1,97 +1,109 @@
# Data Agreement File for RNA-seq project $Revision$
# This file specifies the data agreement between your lab and
# the DCC. It should not be changed unilaterally.
# Lab and general info
grant Wold
lab Caltech
dataType RnaSeq
variables cell, localization, rnaExtract, mapAlgorithm, readType
assembly hg18
dafVersion 0.2.2
# Track/view definition
view Signal
longLabelPrefix Caltech RNA-Seq Map Algorithm Signal
type wig
hasReplicates no
required no
view RawSignal
longLabelPrefix Caltech RNA-Seq Raw Signal
type wig
hasReplicates yes
required no
view Splices
longLabelPrefix Caltech RNA-Seq Map Algorithm Splice Alignments
type bed 12
hasReplicates yes
required no
view Aligns
longLabelPrefix Caltech RNA-Seq Map Algorithm Alignments
type bed 12
hasReplicates yes
required no
view Paired
longLabelPrefix Caltech RNA-Seq Map Algorithm Paired Alignments
type bed 12
hasReplicates yes
required no
# For 2x75 datasets, raw Illumina reads (RawData, fasta format, query75.txt files) are run through bowtie 0.9.8.1 with up to 2 mismatches
# and the resulting mappings are stored (RawData2, bowtie format, hg18sp75spike.bowtie.txt files) for up to ten matches per-read to the genome,
# spiked controls and UCSC knownGene splice junctions. Reads that were not mapped by bowtie (RawData3, fasta format, unmapped.fa files)
# are then mapped onto the genome using blat and filtered using pslReps (RawData4, psl format, bowtierej.hg18.blatbetter files).
# The bowtie and blat mappings are then analyzed by ERANGE3.0.2 to generate wiggles (RawSignal, wiggle format, .graph files),
# bed files of all reads and splices (Aligns, PairedAligns, bed format, all.bed files),
# all bowtie and blat splices (Splices, bed format, allsplices.bed files),
# and blat-only splices (Splices, bed format, blatsplices.bed),
# as well as RPKM expression level measurements at the gene-level (RawData5, rpkm format, final.rpkm),
# exon-level (RawData6, rpkm format, gencode_exon.rpkm), and candidate novel exons (RawData7, rpkm format, accepted.rpkm).
# query75.txt
view RawData
type fasta
hasReplicates yes
required no
# hg18sp75spike.bowtie.txt
view RawData2
type bowtie
hasReplicates yes
required no
# unmapped.fa
view RawData3
type fasta
hasReplicates yes
required no
# bowtierej.hg18.blatbetter
view RawData4
type psl
hasReplicates yes
required no
# final.rpkm
view RawData5
type rpkm
hasReplicates yes
required no
# gencode_exon
view RawData6
type rpkm
hasReplicates yes
required no
# accepted.rpkm
view RawData7
type rpkm
hasReplicates yes
required no
+# hg18splice75.fa
+view RawData8
+type fasta
+hasReplicates no
+required no
+
+# spikes.fa
+view RawData9
+type fasta
+hasReplicates no
+required no
+