src/hg/makeDb/doc/sacCer2.txt 1.6
1.6 2009/04/06 05:42:22 markd
loaded UW yeast footprint tracks
Index: src/hg/makeDb/doc/sacCer2.txt
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/makeDb/doc/sacCer2.txt,v
retrieving revision 1.5
retrieving revision 1.6
diff -b -B -U 4 -r1.5 -r1.6
--- src/hg/makeDb/doc/sacCer2.txt 27 Feb 2009 19:24:53 -0000 1.5
+++ src/hg/makeDb/doc/sacCer2.txt 6 Apr 2009 05:42:22 -0000 1.6
@@ -1241,4 +1241,33 @@
hgcentraltest
# test it with some sequence
############################################################################
+# uwFootprints: (2009-04-04 markd)
+# lifted sacCer1 tag count data and set to Nobel lab
+# William Stafford Noble <noble@gs.washington.edu>
+# Xiaoyu Chen <xchen@cs.washington.edu>
+
+ # lift counts to sacSer2 and give back to UW
+ cd /hive/data/genomes/sacCer1/bed/uwFootprintsxi
+ wget http://noble.gs.washington.edu/proj/footprinting/yeast.dnaseI.tagCounts.bed
+ liftOver yeast.dnaseI.tagCounts.bed /hive/data/genomes/sacCer1/bed/blat.sacCer2.2009-02-04/sacCer1ToSacCer2.over.chain.gz /cluster/home/markd/public_html/tmp/yeast/yeast.dnaseI.tagCounts.sacCer2.bed /cluster/home/markd/public_html/tmp/yeast/yeast.dnaseI.tagCounts.sacCer2.nolift.bed
+
+ mkdir /hive/data/genomes/sacCer2/bed/uwFootprints
+ cd /hive/data/genomes/sacCer2/bed/uwFootprints
+
+ # get back wig
+ wget http://noble.gs.washington.edu/proj/footprinting/yeast.dnaseI.tagCounts.sacCer2.lifted.wig
+
+ # lift other BEDs
+ liftOver /hive/data/genomes/sacCer1/bed/uwFootprints/yeast.mappability.bed /hive/data/genomes/sacCer1/bed/blat.sacCer2.2009-02-04/sacCer1ToSacCer2.over.chain.gz yeast.mappability.bed yeast.mappability.nolift.bed
+ # drop yeast.footprints.bed and truncate name to three decimal places
+ tawk 'NR>1{print $1,$2,$3,sprintf("%0.3f",$4)}' /hive/data/genomes/sacCer1/bed/uwFootprints/yeast.footprints.bed | liftOver stdin /hive/data/genomes/sacCer1/bed/blat.sacCer2.2009-02-04/sacCer1ToSacCer2.over.chain.gz yeast.footprints.bed yeast.footprints.nolift.bed
+
+ wigEncode yeast.dnaseI.tagCounts.sacCer2.lifted.wig uwFootprintsTagCounts.wig uwFootprintsTagCounts.wib
+ # Converted yeast.dnaseI.tagCounts.sacCer2.lifted.wig, upper limit 13798.00, lower limit 1.00
+
+ ln -sf /hive/data/genomes/sacCer2/bed/uwFootprints/uwFootprintsTagCounts.wib /gbdb/sacCer2/wib
+ hgLoadWiggle -tmpDir=/data/tmp sacCer2 uwFootprintsTagCounts uwFootprintsTagCounts.wig
+
+ hgLoadBed -tab -tmpDir=/data/tmp sacCer2 uwFootprintsMappability yeast.mappability.bed
+ hgLoadBed -tmpDir=/data/tmp sacCer2 uwFootprintsPrints yeast.footprints.bed