src/hg/makeDb/doc/monDom5.txt 1.11
1.11 2009/07/02 22:21:45 hiram
Rerun all the equCab2 chains and nets to clean up problems
Index: src/hg/makeDb/doc/monDom5.txt
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/makeDb/doc/monDom5.txt,v
retrieving revision 1.10
retrieving revision 1.11
diff -b -B -U 4 -r1.10 -r1.11
--- src/hg/makeDb/doc/monDom5.txt 23 Jun 2009 23:20:21 -0000 1.10
+++ src/hg/makeDb/doc/monDom5.txt 2 Jul 2009 22:21:45 -0000 1.11
@@ -786,4 +786,67 @@
# validate search expression
hgc-sql -Ne 'select name from nscanGene' monDom5 | egrep -v -e '^chr[0-9a-zA-Z_]+\.[0-9]+\.[0-9]+(\.[0-9a-z]+)?$' |wc -l
#########################################################################
+# lastz Horse equCab2 (DONE - 2009-06-29,07-02 - Hiram)
+ mkdir /hive/data/genomes/monDom5/bed/lastzEquCab2.2009-06-29
+ cd /hive/data/genomes/monDom5/bed/lastzEquCab2.2009-06-29
+
+ cat << '_EOF_' > DEF
+# opossum vs. Horse
+
+# settings for more distant organism alignments
+BLASTZ_H=2000
+BLASTZ_Y=3400
+BLASTZ_L=10000
+BLASTZ_K=2200
+BLASTZ_M=50
+BLASTZ_Q=/scratch/data/blastz/HoxD55.q
+
+# TARGET: Opossum (monDom5)
+SEQ1_DIR=/scratch/data/monDom5/monDom5.2bit
+SEQ1_LEN=/scratch/data/monDom5/chrom.sizes
+SEQ1_CHUNK=10000000
+SEQ1_LAP=10000
+
+# QUERY: Horse equCab2
+SEQ2_DIR=/scratch/data/equCab2/equCab2.2bit
+SEQ2_LEN=/scratch/data/equCab2/chrom.sizes
+SEQ2_CTGDIR=/hive/data/genomes/equCab2/equCab2.UnScaffolds.2bit
+SEQ2_CTGLEN=/hive/data/genomes/equCab2/equCab2.UnScaffolds.sizes
+SEQ2_LIFT=/hive/data/genomes/equCab2/jkStuff/equCab2.chrUn.lift
+SEQ2_CHUNK=20000000
+SEQ2_LIMIT=100
+SEQ2_LAP=0
+
+BASE=/hive/data/genomes/monDom5/bed/lastzEquCab2.2009-06-29
+TMPDIR=/scratch/tmp
+'_EOF_'
+ # << happy emacs
+
+ time doBlastzChainNet.pl `pwd`/DEF \
+ -noLoadChainSplit -verbose=2 -bigClusterHub=swarm \
+ -workhorse=hgwdev \
+ -chainMinScore=5000 -chainLinearGap=loose > do.log 2>&1 &
+ # real 1353m4.161s
+ # had some trouble with the first kluster run due to various factors
+ # not the least of which was a major power outage
+ time doBlastzChainNet.pl `pwd`/DEF \
+ -noLoadChainSplit -verbose=2 -bigClusterHub=swarm \
+ -continue=cat -workhorse=hgwdev \
+ -chainMinScore=5000 -chainLinearGap=loose > cat.log 2>&1 &
+ # real 957m25.164s
+ cat fb.monDom5.chainEquCab2Link.txt
+ # 355004426 bases of 3501660299 (10.138%) in intersection
+
+ mkdir /hive/data/genomes/equCab2/bed/blastz.monDom5.swap
+ cd /hive/data/genomes/equCab2/bed/blastz.monDom5.swap
+ time doBlastzChainNet.pl \
+ /hive/data/genomes/monDom5/bed/lastzEquCab2.2009-06-29/DEF \
+ -noLoadChainSplit -verbose=2 -bigClusterHub=swarm \
+ -swap -workhorse=hgwdev \
+ -chainMinScore=5000 -chainLinearGap=loose > swap.log 2>&1 &
+ # real 320m3.573s
+ cat fb.equCab2.chainMonDom5Link.txt
+ # 351787662 bases of 2428790173 (14.484%) in intersection
+
+#########################################################################