src/hg/makeDb/doc/tetNig2.txt 1.5

1.5 2009/08/11 17:16:53 hiram
done with hg19 tetNig2 chain and net
Index: src/hg/makeDb/doc/tetNig2.txt
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/makeDb/doc/tetNig2.txt,v
retrieving revision 1.4
retrieving revision 1.5
diff -b -B -U 1000000 -r1.4 -r1.5
--- src/hg/makeDb/doc/tetNig2.txt	7 Aug 2009 14:42:33 -0000	1.4
+++ src/hg/makeDb/doc/tetNig2.txt	11 Aug 2009 17:16:53 -0000	1.5
@@ -1,379 +1,554 @@
 # for emacs: -*- mode: sh; -*-
 
 
 # Tetraodon Nigirividis from Genoscope, version v8 (released Mar 2007)
 #    Project website:
 #    http://www.genoscope.cns.fr/externe/Download/Projets/Projet_C/genomique/goldenpath_v2/
 
 ##########################################################################
 # Download sequence (DONE - 2009-08-06 - Hiram)
     mkdir /hive/data/genomes/tetNig2
     cd /hive/data/genomes/tetNig2
     mkdir genoscope
     cd genoscope
 
 D="http://www.genoscope.cns.fr/externe/Download/Projets/Projet_C/genomique/golde
 npath_v2/"
 for F in chr.agp chr.agp.info
 do
     wget --timestamping "${D}/${F}"
 done
 
 for C in 1 1_random 2 2_random 3 4 5 6 7 8 9 10 11 12 13 14 15 \
         15_random 16 17 18 19 20 21 21_random Un_random
 do
     wget --timestamping "${D}/unmasked/chr${C}.fa.bz2"
 done
 
 for F in Tetraodon_mRNAs_v8.2.fa Tetraodon_peptide_v8.2.fa \
 	Tetraodon_peptide_v8.2.fa.compo annotation_tetraodon_v8.2.gff         
 do
     wget --timestamping "${D}/annotation_v8.2/${F}"
 done
 
     bunzip *.fa.bz2
     gzip *.fa
 
 ##########################################################################
 # Initial browser (DONE - 2009-08-06 - Hiram)
     cd /hive/data/genomes/tetNig2
     cat << '_EOF_' > tetNig2.config.ra
 # Config parameters for makeGenomeDb.pl:
 db tetNig2
 clade vertebrate
 scientificName Tetraodon nigroviridis
 commonName Tetraodon
 assemblyDate Mar. 2007
 assemblyLabel Genoscope Tetraodon v8.0 (NCBI project 12350, CAAE01000000)
 orderKey 459
 mitoAcc NC_007176
 fastaFiles /hive/data/genomes/tetNig2/genoscope/chr*.fa.gz
 agpFiles /hive/data/genomes/tetNig2/genoscope/chr.agp
 # qualFiles none
 dbDbSpeciesDir tetraodon
 taxId 99883
 '_EOF_'
     # << happy emacs
 
     time nice -n +19 makeGenomeDb.pl -verbose=2 -workhorse=hgwdev \
 	-noGoldGapSplit -stop=agp tetNig2.config.ra > agp.log 2>&1
 
     time nice -n +19 makeGenomeDb.pl -verbose=2 -workhorse=hgwdev \
 	-noGoldGapSplit -continue=db -stop=db tetNig2.config.ra > db.log 2>&1
 
     time nice -n +19 makeGenomeDb.pl -verbose=2 -workhorse=hgwdev \
 	-noGoldGapSplit -continue=dbDb tetNig2.config.ra > dbDb.log 2>&1
 
     #	add the trackDb files to the source tree and entry to trackDb/makefile
 
 ##########################################################################
 # Repeat Masker (DONE - 2009-08-06 - Hiram)
     mkdir /hive/data/genomes/tetNig2/bed/repeatMasker
     cd /hive/data/genomes/tetNig2/bed/repeatMasker
     doRepeatMasker.pl -verbose=2 -workhorse=hgwdev \
 	-noSplit -buildDir=`pwd` tetNig2 > do.log 2>&1
     cat faSize.rmsk.txt
 # 358618246 bases (56303458 N's 302314788 real 292078336 upper 10236452 lower)
 # in 27 sequences in 1 files
 # %2.85 masked total, %3.39 masked real
 
     # since this doesn't mask very much, use windowmasker instead
     hgsql -e "drop table rmsk;" tetNig2
     #	this leaves the interrupted repeats track showing on genome-test
 
 ########################################################################
 # Simple Repeats (DONE - 2009-08-06 - Hiram)
     mkdir /hive/data/genomes/tetNig2/bed/simpleRepeat
     cd /hive/data/genomes/tetNig2/bed/simpleRepeat
     doSimpleRepeat.pl -workhorse=hgwdev \
 	-buildDir=`pwd` tetNig2 > do.log 2>&1 &
     #	fails on the job for chrM, make an empty result:
     touch /hive/data/genomes/tetNig2/TrfPart/009/009.lst.bed
     doSimpleRepeat.pl -workhorse=hgwdev -continue=filter \
 	-buildDir=`pwd` tetNig2 > filter.log 2>&1 &
     cat fb.simpleRepeat 
     #	11549259 bases of 332311746 (3.475%) in intersection
 
 ########################################################################
+# Marking *all* gaps - they are not all in the AGP file
+#	(DONE - 2009-08-07 - Hiram)
+    mkdir /hive/data/genomes/tetNig2/bed/allGaps
+    time nice -n +19 findMotif -motif=gattaca -verbose=4 \
+	-strand=+ ../../tetNig2.2bit > findMotif.txt 2>&1
+    #	real    0m7.967s
+    grep "^#GAP " findMotif.txt | sed -e "s/^#GAP //" > allGaps.bed
+    featureBits tetNig2 -not gap -bed=notGap.bed
+    featureBits tetNig2 allGaps.bed notGap.bed -bed=net.gaps.bed
+    #	what is the last index in the existing gap table:
+    hgsql -N -e "select ix from gap;" tetNig2 | sort -n | tail -1
+    #	34284
+    cat << '_EOF_' > mkGap.pl
+#!/usr/bin/env perl
+
+use strict;
+use warnings;
+
+my $ix=`hgsql -N -e "select ix from gap;" tetNig2 | sort -n | tail -1`;
+chomp $ix;
+
+open (FH,"<new.gaps.bed") or die "can not read new.gaps.bed";
+while (my $line = <FH>) {
+    my ($chrom, $chromStart, $chromEnd, $rest) = split('\s+', $line);
+    ++$ix;
+    printf "%s\t%d\t%d\t%d\tN\t%d\tother\tyes\n", $chrom, $chromStart,
+        $chromEnd, $ix, $chromEnd-$chromStart;
+}
+close (FH);
+'_EOF_'
+    # << happy emacs
+    chmod +x ./mkGap.pl
+    ./mkGap.pl > other.gap
+    hgLoadBed -sqlTable=$HOME/kent/src/hg/lib/gap.sql \
+	-noLoad tetNig2 otherGap other.gap
+    #	Loaded 17051
+    #	adding this many:
+    wc -l bed.tab
+    #	17051
+    #	starting with this many
+    hgsql -e "select count(*) from gap;" tetNig2
+    #	17140
+    hgsql tetNig2 -e 'load data local infile "bed.tab" into table gap;'
+    #	result count:
+    hgsql -e "select count(*) from gap;" tetNig2
+    #	34191
+    # == 17140 + 17051
+
+########################################################################
 # WindowMasker (DONE - 2009-08-06 - Hiram)
     mkdir /hive/data/genomes/tetNig2/bed/windowMasker
     cd /hive/data/genomes/tetNig2/bed/windowMasker
     doWindowMasker.pl -workhorse=hgwdev -buildDir=`pwd` tetNig2 > do.log 2>&1
     twoBitToFa tetNig2.wmsk.sdust.2bit stdout | faSize stdin
     #	358618246 bases (56303458 N's 302314788 real 241249522 upper
     #	61065266 lower) in 27 sequences in 1 files
     #	%17.03 masked total, %20.20 masked real
 
     #	load this initial data to get ready to clean it
     ssh hgwdev
     cd /hive/data/genomes/tetNig2/bed/windowMasker
     hgLoadBed tetNig2 windowmaskerSdust windowmasker.sdust.bed.gz
     #	Loaded 1649525 elements of size 3
     featureBits -countGaps tetNig2 windowmaskerSdust
     #	117367586 bases of 358618246 (32.728%) in intersection
 
     #	eliminate the gaps from the masking
     featureBits tetNig2 -not gap -bed=notGap.bed
+    #	302314788 bases of 302314788 (100.000%) in intersection
     time nice -n +19 featureBits tetNig2 windowmaskerSdust notGap.bed \
         -bed=stdout | gzip -c > cleanWMask.bed.gz
-    #	91061086 bases of 332311746 (27.402%) in intersection
+    #	61065266 bases of 302314788 (20.199%) in intersection
     #	reload track to get it clean
     hgLoadBed tetNig2 windowmaskerSdust cleanWMask.bed.gz
-    #	Loaded 1647549 elements of size 4
+    #	Loaded 1644977 elements of size 4
     featureBits -countGaps tetNig2 windowmaskerSdust
-    #	91061086 bases of 358618246 (25.392%) in intersection
+    #	61065266 bases of 358618246 (17.028%) in intersection
 
     #	mask the sequence with this clean mask
     zcat cleanWMask.bed.gz \
 	| twoBitMask ../../tetNig2.unmasked.2bit stdin \
 	    -type=.bed tetNig2.cleanWMSdust.2bit
     twoBitToFa tetNig2.cleanWMSdust.2bit stdout | faSize stdin \
         > tetNig2.cleanWMSdust.faSize.txt
     cat tetNig2.cleanWMSdust.faSize.txt
     #	358618246 bases (56303458 N's 302314788 real 241249522 upper 61065266
     #	lower) in 27 sequences in 1 files
     #	%17.03 masked total, %20.20 masked real
 
 ########################################################################
 # MASK SEQUENCE WITH WM+TRF (DONE - 2009-08-06 - Hiram)
     cd /hive/data/genomes/tetNig2
     twoBitMask -add bed/windowMasker/tetNig2.cleanWMSdust.2bit \
 	bed/simpleRepeat/trfMask.bed tetNig2.2bit
     #	safe to ignore the warnings about BED file with >=13 fields
     twoBitToFa tetNig2.2bit stdout | faSize stdin > faSize.tetNig2.txt
-    cat faSize.tetNig2.wmskSdust.TRF.txt
+    cat faSize.tetNig2.txt
     #	358618246 bases (56303458 N's 302314788 real 241039472 upper 61275316
     #	lower) in 27 sequences in 1 files
     #	%17.09 masked total, %20.27 masked real
 
     #	create symlink to gbdb
     ssh hgwdev
     rm /gbdb/tetNig2/tetNig2.2bit
     ln -s `pwd`/tetNig2.2bit /gbdb/tetNig2/tetNig2.2bit
 
 #########################################################################
 # MAKE 11.OOC FILE FOR BLAT/GENBANK (DONE - 2009-08-06 - Hiram)
     # Use -repMatch=130 (based on size -- for human we use 1024, and 
     # Tetraodon size is ~12.7% of human judging by gapless tetNig2 vs.
     #	hg18 genome size from featureBits.
     # genome.  Bump that up a bit to be more conservative.
     #	100*302314788/2897310462 = 10.434324
 
     cd /hive/data/genomes/tetNig2
     blat tetNig2.2bit /dev/null /dev/null -tileSize=11 \
       -makeOoc=jkStuff/tetNig2.11.ooc -repMatch=130
     #	Wrote 8132 overused 11-mers to jkStuff/tetNig2.11.ooc
 
     #	copy all of this stuff to the klusters:
+    cd /hive/data/genomes/tetNig2/jkStuff
+    gapToLift tetNig2 nonBridged.lift -bedFile=nonBridged.bed
     cd /hive/data/genomes/tetNig2
     mkdir /hive/data/staging/data/tetNig2
-    cp -p jkStuff/tetNig2.11.ooc chrom.sizes tetNig2.2bit \
-	/hive/data/staging/data/tetNig2
+    cp -p jkStuff/tetNig2.11.ooc jkStuff/nonBridged.lift \
+	chrom.sizes tetNig2.2bit /hive/data/staging/data/tetNig2
 
 #########################################################################
 # Ensembl genes v55 (DONE - 2009-08-06 - Hiram)
     cd /hive/data/genomes/tetNig2
     cat << '_EOF_' > tetNig2.ensGene.ra
 # required db variable
 db tetNig2
 # optional nameTranslation, the sed command that will transform
 #       Ensemble names to UCSC names.  With quotes just to make sure.
 nameTranslation "s/^\([0-9XY][0-9]*\)/chr\1/; s/^MT/chrM/; s/^Un/chrUn/"
 '_EOF_'
 #  << happy emacs
 
     doEnsGeneUpdate.pl -verbose=2 -workhorse=hgwdev \
 	-ensVersion=55 -stop=process tetNig2.ensGene.ra > tetNig2.55.log 2>&1
     doEnsGeneUpdate.pl -verbose=2 -workhorse=hgwdev \
 	-ensVersion=55 -continue=load tetNig2.ensGene.ra >> tetNig2.55.log 2>&1
 
     featureBits tetNig2 ensGene
     # 31637658 bases of 332311746 (9.520%) in intersection
 
 #########################################################################
 #  BLATSERVERS ENTRY (DONE - 2009-08-06 - Hiram)
 Thu Aug  6 15:58:09 PDT 2009
 #	After getting a blat server assigned by the Blat Server Gods,
     ssh hgwdev
 
     hgsql -e 'INSERT INTO blatServers (db, host, port, isTrans, canPcr) \
 	VALUES ("tetNig2", "blat15", "17794", "1", "0"); \
 	INSERT INTO blatServers (db, host, port, isTrans, canPcr) \
 	VALUES ("tetNig2", "blat15", "17795", "0", "1");' \
 	    hgcentraltest
     #	test it with some sequence
 
 #########################################################################
 # Set default position same as tetNig1 after blating tetNig1 to here
 #		(DONE - 2009-08-06 - Hiram)
     hgsql -e \
 'update dbDb set defaultPos="chr10:2797091-2804214" where name="tetNig2";' \
         hgcentraltest
 
 #########################################################################
 # HUMAN (hg18) PROTEINS TRACK (DONE 2009-08-07 braney )
 
 ### !!!! needs re-doing to deal with chrUn correctly !!!!
 
     # bash  if not using bash shell already
 
     cd /cluster/data/tetNig2
     mkdir /cluster/data/tetNig2/blastDb
 
     awk '{if ($2 > 1000000) print $1}' chrom.sizes > 1meg.lst
     twoBitToFa -seqList=1meg.lst  tetNig2.2bit temp.fa
     faSplit gap temp.fa 1000000 blastDb/x -lift=blastDb.lft
     rm temp.fa 1meg.lst
 
     awk '{if ($2 <= 1000000) print $1}' chrom.sizes > less1meg.lst
     twoBitToFa -seqList=less1meg.lst  tetNig2.2bit temp.fa
     faSplit about temp.fa 1000000 blastDb/y 
     rm temp.fa less1meg.lst
 
     cd blastDb
     for i in *.fa
     do
 	/hive/data/outside/blast229/formatdb -i $i -p F
     done
     rm *.fa
     ls *.nsq | wc -l
 # 394
 
 
     mkdir -p /cluster/data/tetNig2/bed/tblastn.hg18KG
     cd /cluster/data/tetNig2/bed/tblastn.hg18KG
     echo  ../../blastDb/*.nsq | xargs ls -S | sed "s/\.nsq//"  > query.lst
     wc -l query.lst
 # 394 query.lst
 
    # we want around 100000 jobs
    calc `wc /cluster/data/hg18/bed/blat.hg18KG/hg18KG.psl | awk '{print $1}'`/\(100000/`wc query.lst | awk '{print $1}'`\)
 
 # 36727/(100000/394) = 144.704380
 
    mkdir -p kgfa
    split -l 145 /cluster/data/hg18/bed/blat.hg18KG/hg18KG.psl  kgfa/kg
    cd kgfa
    for i in *; do 
      nice pslxToFa $i $i.fa; 
      rm $i; 
    done
    cd ..
    ls -1S kgfa/*.fa > kg.lst
    wc kg.lst
 #  254  254 3302 kg.lst
 
    mkdir -p blastOut
    for i in `cat kg.lst`; do  mkdir blastOut/`basename $i .fa`; done
    tcsh
    cd /cluster/data/tetNig2/bed/tblastn.hg18KG
    cat << '_EOF_' > blastGsub
 #LOOP
 blastSome $(path1) {check in line $(path2)} {check out exists blastOut/$(root2)/q.$(root1).psl }
 #ENDLOOP
 '_EOF_'
 
    cat << '_EOF_' > blastSome
 #!/bin/sh
 BLASTMAT=/hive/data/outside/blast229/data
 export BLASTMAT
 g=`basename $2`
 f=/tmp/`basename $3`.$g
 for eVal in 0.01 0.001 0.0001 0.00001 0.000001 1E-09 1E-11
 do
 if /hive/data/outside/blast229/blastall -M BLOSUM80 -m 0 -F no -e $eVal -p tblastn -d $1 -i $2 -o $f.8
 then
         mv $f.8 $f.1
         break;
 fi
 done
 if test -f  $f.1
 then
     if /cluster/bin/i386/blastToPsl $f.1 $f.2
     then
 	liftUp -nosort -type=".psl" -nohead $f.3 /cluster/data/tetNig2/blastDb.lft carry $f.2
         liftUp -nosort -type=".psl" -pslQ -nohead $3.tmp /cluster/data/hg18/bed/blat.hg18KG/protein.lft warn $f.3
         if pslCheck -prot $3.tmp
         then                  
             mv $3.tmp $3     
             rm -f $f.1 $f.2 $f.3 $f.4
         fi
         exit 0               
     fi                      
 fi                         
 rm -f $f.1 $f.2 $3.tmp $f.8 $f.3 $f.4
 exit 1
 '_EOF_'
     # << happy emacs
     chmod +x blastSome
     exit 
     
     ssh swarm
     cd /cluster/data/tetNig2/bed/tblastn.hg18KG
     gensub2 query.lst kg.lst blastGsub blastSpec
     para create blastSpec
 #    para try, check, push, check etc.
 
     para time
 # Completed: 100076 of 100076 jobs
 # CPU time in finished jobs:    2219419s   36990.31m   616.51h   25.69d  0.070 y
 # IO & Wait Time:                445463s    7424.39m   123.74h    5.16d  0.014 y
 # Average job time:                  27s       0.44m     0.01h    0.00d
 # Longest finished job:             319s       5.32m     0.09h    0.00d
 # Submission to last job:          2791s      46.52m     0.78h    0.03d
 
     ssh swarm
     cd /cluster/data/tetNig2/bed/tblastn.hg18KG
     mkdir chainRun
     cd chainRun
     tcsh
     cat << '_EOF_' > chainGsub
 #LOOP
 chainOne $(path1)
 #ENDLOOP
 '_EOF_'
 
     cat << '_EOF_' > chainOne
 (cd $1; cat q.*.psl | simpleChain -prot -outPsl -maxGap=12000 stdin ../c.`basename $1`.psl)
 '_EOF_'
     chmod +x chainOne
     ls -1dS ../blastOut/kg?? > chain.lst
     gensub2 chain.lst single chainGsub chainSpec
     # do the cluster run for chaining
     para create chainSpec
     para try, check, push, check etc.
 
 # Completed: 254 of 254 jobs
 # CPU time in finished jobs:     590077s    9834.62m   163.91h    6.83d  0.019 y
 # IO & Wait Time:                 20749s     345.81m     5.76h    0.24d  0.001 y
 # Average job time:                2405s      40.08m     0.67h    0.03d
 # Longest finished job:           36592s     609.87m    10.16h    0.42d
 # Submission to last job:         36604s     610.07m    10.17h    0.42d
 
     cd /cluster/data/tetNig2/bed/tblastn.hg18KG/blastOut
     for i in kg??
     do
        cat c.$i.psl | awk "(\$13 - \$12)/\$11 > 0.6 {print}" > c60.$i.psl
        sort -rn c60.$i.psl | pslUniq stdin u.$i.psl
        awk "((\$1 / \$11) ) > 0.60 { print   }" c60.$i.psl > m60.$i.psl
        echo $i
     done
     sort u.*.psl m60* | uniq | sort -T /tmp -k 14,14 -k 16,16n -k 17,17n > ../blastHg18KG.psl
     cd ..
     pslCheck blastHg18KG.psl
 # checked: 38517 failed: 0 errors: 0
 
     # load table 
     ssh hgwdev
     cd /cluster/data/tetNig2/bed/tblastn.hg18KG
     hgLoadPsl tetNig2 blastHg18KG.psl
 
     # check coverage
     featureBits tetNig2 blastHg18KG 
 # 18646943 bases of 332311746 (5.611%) in intersection
 
     featureBits tetNig2 blastHg18KG ensGene  -enrichment
 # blastHg18KG 5.611%, ensGene 9.520%, both 4.993%, cover 88.97%, enrich 9.35x
 
     rm -rf blastOut
 #end tblastn
+
+#########################################################################
+# Creating contigs 2bit file (DONE - 2009-08-10 - Hiram)
+    cd /hive/data/genomes/tetNig2/jkStuff
+    gapToLift tetNig2 nonBridged.lift -bedFile=nonBridged.bed
+    #	there aren't any gaps in chrM and it doesn't show up in
+    #	the result !  Add chrM to this lift file:
+    echo -e "0\tchrM.00\t16462\tchrM\t16462" >> nonBridged.lift
+    echo -e "chrM\t0\t16462\tchrM.00" >> nonBridged.bed
+
+    #	take a look at the nonBridged.bed as a custom track
+    mkdir /hive/data/genomes/tetNig2/contigs
+    cd /hive/data/genomes/tetNig2/contigs
+    ln -s ../jkStuff/nonBridged.lift .
+    ~/kent/src/hg/utils/lft2BitToFa.pl ../tetNig2.2bit nonBridged.lift \
+	| gzip -c > tetNig2.contigs.fa.gz
+    #	make sure nothing was destroyed:
+    faCount *.fa.gz > faCount.contigs.txt 2>&1
+    twoBitToFa ../tetNig2.2bit stdout | faCount stdin > faCount.2bit.txt 2>&1
+    tail -1 faCount.contigs.txt
+# total   333797246       81050307        70114488        70126181
+# 81023812	31482458        9799877
+    tail -1 faCount.2bit.txt
+# total   358618246       81050307        70114488        70126181
+# 81023812	56303458        9799877
+    #	only the total size and N count are different
+
+    faSize tetNig2.contigs.fa.gz
+    #	333797246 bases (31482458 N's 302314788 real 241039472 upper 61275316
+    #	lower) in 14352 sequences in 1 files
+    #	%18.36 masked total, %20.27 masked real
+    twoBitToFa ../tetNig2.2bit stdout | faSize stdin
+    #	358618246 bases (56303458 N's 302314788 real 241039472 upper 61275316
+    #	 lower) in 27 sequences in 1 files
+    #	%17.09 masked total, %20.27 masked real
+
+    faToTwoBit tetNig2.contigs.fa.gz tetNig2.contigs.2bit
+    twoBitInfo tetNig2.contigs.2bit stdout | sort -k2nr > tetNig2.contigs.sizes
+
+    cp -p tetNig2.contigs.2bit tetNig2.contigs.sizes  \
+	/hive/data/staging/data/tetNig2
+    cp -p nonBridged.lift /hive/data/staging/data/tetNig2/tetNig2.contigs.lift
+
+#########################################################################
+# GENBANK AUTO UPDATE (DONE - 2009-08-11 - Hiram)
+    # align with latest genbank process.
+    ssh hgwdev
+    cd ~/kent/src/hg/makeDb/genbank
+    cvsup
+    # edit etc/genbank.conf to add tetNig2 just before tetNig1
+
+# tetNig2 (Tetraodon)
+tetNig2.serverGenome = /hive/data/genomes/tetNig2/tetNig2.2bit
+tetNig2.clusterGenome = /scratch/data/tetNig2/tetNig2.2bit
+tetNig2.ooc = /scratch/data/tetNig2/tetNig2.11.ooc
+tetNig2.lift = /scratch/data/tetNig2/tetNig2.contigs.lift
+tetNig2.align.unplacedChroms = chr*_random
+tetNig2.refseq.mrna.native.pslCDnaFilter  = ${lowCover.refseq.mrna.native.pslCDnaFilter}
+tetNig2.refseq.mrna.xeno.pslCDnaFilter    = ${lowCover.refseq.mrna.xeno.pslCDnaFilter}  
+tetNig2.genbank.mrna.native.pslCDnaFilter = ${lowCover.genbank.mrna.native.pslCDnaFilter}
+tetNig2.genbank.mrna.xeno.pslCDnaFilter   = ${lowCover.genbank.mrna.xeno.pslCDnaFilter}
+tetNig2.genbank.est.native.pslCDnaFilter  = ${lowCover.genbank.est.native.pslCDnaFilter}
+tetNig2.downloadDir = tetNig2
+tetNig2.genbank.mrna.xeno.loadDesc = yes
+tetNig2.refseq.mrna.native.load = no
+
+    cvs ci -m "Added tetNig2" etc/genbank.conf
+    # update /cluster/data/genbank/:
+    make etc-update
+
+    ssh genbank
+    screen	#	use a screen to manage this job
+    cd /cluster/data/genbank
+    time nice -n +19 bin/gbAlignStep -initial tetNig2 &
+    #	logFile: var/build/logs/2009.08.10-16:42:06.tetNig2.initalign.log
+    #	real    578m42.777s
+
+    # load database when finished
+    ssh hgwdev
+    cd /cluster/data/genbank
+    time nice -n +19 ./bin/gbDbLoadStep -drop -initialLoad tetNig2
+    #	logFile: var/dbload/hgwdev/logs/2009.08.11-09:22:29.dbload.log
+    #	real    31m29.282s
+
+    # enable daily alignment and update of hgwdev
+    cd ~/kent/src/hg/makeDb/genbank
+    cvsup
+    # add tetNig2 to:
+        etc/align.dbs
+        etc/hgwdev.dbs
+    cvs ci -m "Added tetNig2 - Tetraodon Nigirividis" \
+	etc/align.dbs etc/hgwdev.dbs
+    make etc-update
+
+#########################################################################
+# lastz swap Human hg19 (DONE - 2009-08-11 - Hiram)
+    #	result of human alignment  (date stamp on directory is incorrect)
+    cd /hive/data/genomes/hg19/bed/lastzTetNig2.2009-10-10
+    cat fb.hg19.chainTetNig2Link.txt 
+    #	49611132 bases of 2897316137 (1.712%) in intersection
+
+    #	running the swap
+    mkdir /hive/data/genomes/tetNig2/bed/blastz.hg19.swap
+    cd /hive/data/genomes/tetNig2/bed/blastz.hg19.swap
+    time nice -n +19 doBlastzChainNet.pl -verbose=2 \
+	/hive/data/genomes/hg19/bed/lastzTetNig2.2009-10-10/DEF \
+	-noLoadChainSplit -chainMinScore=5000 -chainLinearGap=loose \
+	-workhorse=hgwdev -smallClusterHub=memk -bigClusterHub=swarm \
+	-swap > swap.log 2>&1 &
+    #	real    13m21.591s
+    #	forgot the qRepeats for tetNig2
+    rm axtChain/tetNig2.hg19.net
+    time nice -n +19 doBlastzChainNet.pl -verbose=2 \
+	/hive/data/genomes/hg19/bed/lastzTetNig2.2009-10-10/DEF \
+	-continue=load -qRepeats=windowmaskerSdust \
+	-noLoadChainSplit -chainMinScore=5000 -chainLinearGap=loose \
+	-workhorse=hgwdev -smallClusterHub=memk -bigClusterHub=swarm \
+	-swap > load.log 2>&1 &
+    #	real    4m7.559s
+    cat fb.tetNig2.chainHg19Link.txt 
+    #	42910930 bases of 302314788 (14.194%) in intersection
+
+##############################################################################