src/hg/inc/bamFile.h 1.9
1.9 2009/09/23 23:50:30 angie
Added bamShowFlagsEnglish to explain the flag bits.
Index: src/hg/inc/bamFile.h
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/inc/bamFile.h,v
retrieving revision 1.8
retrieving revision 1.9
diff -b -B -U 1000000 -r1.8 -r1.9
--- src/hg/inc/bamFile.h 14 Sep 2009 23:44:25 -0000 1.8
+++ src/hg/inc/bamFile.h 23 Sep 2009 23:50:30 -0000 1.9
@@ -1,72 +1,75 @@
/* bamFILE -- interface to binary alignment format files using Heng Li's samtools lib. */
#ifndef BAMFILE_H
#define BAMFILE_H
// bam.h is incomplete without _IOLIB set to 1, 2 or 3. 2 is used by Makefile.generic:
#define _IOLIB 2
#include "bam.h"
#include "sam.h"
void bamIgnoreStrand();
/* Change the behavior of this lib to disregard item strand.
* If called, this should be called before any other bam functions. */
char *bamFileNameFromTable(char *db, char *table, char *bamSeqName);
/* Return file name from table. If table has a seqName column, then grab the
* row associated with bamSeqName (which is not nec. in chromInfo, e.g.
* bam file might have '1' not 'chr1'). */
void bamFetch(char *bamFileName, char *position, bam_fetch_f callbackFunc, void *callbackData);
/* Open the .bam file, fetch items in the seq:start-end position range,
* and call callbackFunc on each bam item retrieved from the file plus callbackData.
* Note: if sequences in .bam file don't begin with "chr" but cart position does, pass in
* cart position + strlen("chr") to match the .bam file sequence names. */
boolean bamIsRc(const bam1_t *bam);
/* Return TRUE if alignment is on - strand. If bamIgnoreStrand has been called,
* then this always returns FALSE. */
INLINE int bamUnpackCigarElement(unsigned int packed, char *retOp)
/* Given an unsigned int containing a number of bases and an offset into an
* array of BAM-enhanced-CIGAR ASCII characters (operations), store operation
* char into *retOp (retOp must not be NULL) and return the number of bases. */
{
// decoding lifted from samtools bam.c bam_format1(), long may it remain stable:
#define BAM_DOT_C_OPCODE_STRING "MIDNSHP"
int n = packed>>BAM_CIGAR_SHIFT;
int opcode = packed & BAM_CIGAR_MASK;
if (opcode >= strlen(BAM_DOT_C_OPCODE_STRING))
errAbort("bamUnpackCigarElement: unrecognized opcode %d. "
"(I only recognize 0..%lu [" BAM_DOT_C_OPCODE_STRING "]) "
"Perhaps samtools bam.c's bam_format1 encoding changed? If so, update me.",
opcode, (unsigned long)(strlen(BAM_DOT_C_OPCODE_STRING)-1));
*retOp = BAM_DOT_C_OPCODE_STRING[opcode];
return n;
}
char *bamGetQuerySequence(const bam1_t *bam);
/* Return the nucleotide sequence encoded in bam. The BAM format
* reverse-complements query sequence when the alignment is on the - strand,
* so here we rev-comp it back to restore the original query sequence. */
char *bamGetCigar(const bam1_t *bam);
/* Return a BAM-enhanced CIGAR string, decoded from the packed encoding in bam. */
void bamShowCigarEnglish(const bam1_t *bam);
/* Print out cigar in English e.g. "20 (mis)Match, 1 Deletion, 3 (mis)Match" */
+void bamShowFlagsEnglish(const bam1_t *bam);
+/* Print out flags in English, e.g. "Mate is on '-' strand; Properly paired". */
+
int bamGetTargetLength(const bam1_t *bam);
/* Tally up the alignment's length on the reference sequence from
* bam's packed-int CIGAR representation. */
struct ffAli *bamToFfAli(const bam1_t *bam, struct dnaSeq *target, int targetOffset);
/* Convert from bam to ffAli format. */
bam1_t *bamClone(const bam1_t *bam);
/* Return a newly allocated copy of bam. */
void bamShowTags(const bam1_t *bam);
/* Print out tags in HTML: bold key, no type indicator for brevity. */
#endif//ndef BAMFILE_H