src/hg/makeDb/doc/metAce1.txt 1.3

1.3 2009/11/25 21:48:40 hiram
change autoScaleDefault to autoScale
Index: src/hg/makeDb/doc/metAce1.txt
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/makeDb/doc/metAce1.txt,v
retrieving revision 1.2
retrieving revision 1.3
diff -b -B -U 1000000 -r1.2 -r1.3
--- src/hg/makeDb/doc/metAce1.txt	26 Jul 2006 16:59:56 -0000	1.2
+++ src/hg/makeDb/doc/metAce1.txt	25 Nov 2009 21:48:40 -0000	1.3
@@ -1,466 +1,466 @@
 # for emacs: -*- mode: sh; -*-
 
  
 # This file describes building the browser database for the archaeal
 # species Methanosarcina acetivorans.
 
 # DOWNLOAD SEQUENCE FROM GENBANK (DONE 8/05)
 
     ssh eieio
     mkdir /cluster/store5/archae/metAce1
     ln -s /cluster/store5/archae/metAce1 /cluster/data/metAce1
     cd /cluster/data/metAce1
     wget ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/Methanosarcina_acetivorans/NC_003552.fna
     mv NC_003552.fna chr.fa
     # Edit header of chr.fa to '> metAce1'
 
 # CREATE DATABASES AND A BUNCH OF INITIAL STUFF (DONE 8/05)
 
     ssh hgwdev
     echo 'create database metAce1' | hgsql ''
     cd /cluster/data/metAce1
     faToNib chr.fa nib/chr.nib
     hgNibSeq metAce1 /cluster/data/metAce1/nib chr.fa
     faSize -detailed chr.fa > chrom.sizes
     mkdir -p /gbdb/metAce1/nib
     echo "create table grp (PRIMARY KEY(NAME)) select * from hg16.grp" \
 	    | hgsql metAce1
     echo 'INSERT INTO dbDb \
         (name, description, nibPath, organism, \
                 defaultPos, active, orderKey, genome, scientificName, \
                 htmlPath, hgNearOk) values \
         ("metAce1", "April 2002", "/gbdb/metAce1/nib", "Methanosarcina acetivorans", \
                "chr:500000-550000", 1, 205, "Methanosarcina acetivorans", \
                 "Methanosarcina acetivorans C2A", "/gbdb/metAce1/html/description.html", \
                 0);' \
       | hgsql hgcentraltest
     echo 'INSERT INTO defaultDb (genome, name) values ("Methanosarcina acetivorans", "metAce1");' \
       | hgsql hgcentraltest
     echo 'INSERT INTO genomeClade (genome, clade, priority) values ("Methanosarcina acetivorans", "archaea",85);'  \
       | hgsql hgcentraltest
 
     cd ~/kent/src/hg/makeDb/trackDb
     # edit the trackDb makefile
 
     # add the trackDb directories
     mkdir -p archae/metAce1
     cvs add archae
     cvs add archae/metAce1
     cvs commit
 
 # GC 20 BASE WIGGLE TRACK (DONE 8/13/05)
 
     mkdir /cluster/data/metAce1/bed/gc20Base
     cd /cluster/data/metAce1/bed/gc20Base
     mkdir wigData20 dataLimits20
     hgGcPercent -chr=chr -file=stdout -win=20 -overlap=19 metAce1 ../../nib | grep -w GC | \
     awk '
 {
     bases = $3 - $2
     perCent = $5/10.0
     printf "%d\t%.1f\n", $2+1, perCent
 }' | wigAsciiToBinary -dataSpan=1 -chrom=chr \
 	-wibFile=wigData20/gc20Base_1 -name=1 stdin > dataLimits20/chr
     hgLoadWiggle metAce1 gc20Base wigData20/*.wig
     mkdir /gbdb/metAce1/wib
     ln -s `pwd`/wigData20/*.wib /gbdb/metAce1/wib
     #	the trackDb entry
 
 # CONTIG TRACK 
     # reformat is a Todd Lowe program
     for num in `seq 746 946`; do   
        curl -o ${num}.gbk "http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?txt=on&val=AE009${num}.1"
        reformat fasta ${num}.gbk >> contigs.fa
     done
     blat /cluster/data/metAce1/chr.fa contigs.fa -minIdentity=100 contigs.psl
     perfectBlatBed4 contigs.psl contigs.bed
     mkdir /cluster/data/metAce1/bed/metAce1Contigs
     cp contigs.bed /cluster/data/metAce1/bed/metAce1Contigs
     cd /cluster/data/metAce1/bed/metAce1Contigs
     hgLoadBed metAce1 metAce1Contigs contigs.bed
     # the trackDb entry: 
 track metAce1Contigs
 shortLabel Contigs
 longLabel Contigs deposited in Genbank
 group map
 priority 0.5
 visibility pack
 url http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=nucleotide&cmd=search&term=$$
 type bed 4 .
 
 
 # TANDEM REPEAT MASKER (DONE)
 
     ssh hgwdev
     mkdir -p /cluster/data/metAce1/bed/simpleRepeat
     cd /cluster/data/metAce1
     trfBig chr.fa /dev/null -bedAt=/cluster/data/metAce1/bed/simpleRepeat/chr.bed
     cd /cluster/data/metAce1/bed/simpleRepeat
     vi chr.bed and replace metAce1 with chr
     hgLoadBed metAce1 simpleRepeat *.bed -sqlTable=~kent/src/hg/lib/simpleRepeat.sql
 
 # DESCRIPTION PAGE (DONE)
 
     ssh hgwdev
     # Write ~/kent/src/hg/makeDb/trackDb/archae/metAce1/description.html
     chmod a+r ~/kent/src/hg/makeDb/trackDb/archae/metAce1/description.html
     # Check it in.
     mkdir /gbdb/metAce1/html
     ln -s /cluster/data/metAce1/html/description.html /gbdb/metAce1/html/
 
 # GENBANK PROTEIN-CODING GENES (DONE)
 
     ssh hgwdev
     mkdir /cluster/data/metAce1/genbank
     cd /cluster/data/metAce1/genbank
     wget ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/Methanosarcina_acetivorans/NC_003552.gbk
     mv NC_003552.gbk metAce1.gbk
     # Create 3 files to assist parsing of the genbank
     # 1. for a genePred file
 
 echo 'locus_tag
 chr
 strand
 start
 end
 start
 end
 1
 start
 end
 lineNumber
 gene
 cmpl
 cmpl
 0' > metAce1-params-gp.txt
     # 2. for the peptide parts
     echo 'locus_tag
 translation' > metAce1-params-pep.txt
     # 3. for the other gene information
     echo 'locus_tag
 gene
 product
 note
 protein_id
 db_xref
 EC_number
 db_xref2' > metAce1-params-xra.txt
     # Now extract the genes and information:
     gbArchaeGenome metAce1.gbk metAce1-params-gp.txt metAce1-genbank-cds.gp
     gbArchaeGenome metAce1.gbk metAce1-params-pep.txt metAce1-genbank-cds.pep
     gbArchaeGenome metAce1.gbk metAce1-params-xra.txt metAce1-genbank-cds.xra
     hgsql metAce1 < /cluster/home/baertsch/kent/src/hg/lib/pepPred.sql
     hgsql metAce1 < /cluster/home/baertsch/kent/src/hg/lib/minGeneInfo.sql
     echo rename table pepPred to gbProtCodePep | hgsql metAce1
     echo rename table minGeneInfo to gbProtCodeXra | hgsql metAce1
     echo load data local infile \'metAce1-genbank-cds.pep\' into table gbProtCodePep | hgsql metAce1
     echo load data local infile \'metAce1-genbank-cds.xra\' into table gbProtCodeXra | hgsql metAce1
     ldHgGene metAce1 refSeq metAce1.gp -predTab -genePredExt
 
 # COG STUFF
   awk 'NR>3{OFS="\t";print $6,$8,$7}' /projects/lowelab/db/Bacteria/Methanosarcina_acetivorans/NC_003552.ptt  > COG
   hgsql metAce1 < /cluster/home/baertsch/kent/src/hg/lib/cogs.sql
   echo "load data local infile 'COG' into table COG" | hgsql metAce1
 # load cog codes
  hgsql metAce1 < /cluster/data/metAce1/genbank/COGXra.sql
 
 
 # GENBANK rRNA GENES (NOT QUITE DONE)
     ssh hgdev
     cd /cluster/data/metAce1/genbank
     gbArchaeGenome -kind=rRNA metAce1.gbk metAce1-params-bed.txt metAce1-rrnas.bed
     echo 'gene product NA' > metAce1-params-rrna-xra.txt
     gbArchaeGenome -kind=rRNA metAce1.gbk metAce1-params-rrna-xra.txt metAce1-rrnas-xra.txt
     hgLoadBed metAce1 gbRRNA metAce1-rrnas.bed
     hgsql metAce1 < ~/kent/src/hg/lib/minGeneInfo.sql
     echo rename table minGeneInfo to gbRRNAXra | hgsql metAce1
     echo load data local infile \'metAce1-rrnas-xra.txt\' into table gbRRNAXra | hgsql metAce1
 
 
 # TODD LOWE tRNA GENES ()
 
     # This one is a bed 6+ file created by hand of 46 tRNAs and 1 pseudo tRNA by Todd
     # Lowe.  See ~/kent/src/hg/lib/loweTrnaGene.as for a description of the fields.
     # **Showing the tRNAScanSE instructions would be nice in the future.  
     ssh hgwdev
     mkdir /cluster/data/metAce1/bed/loweTrnaGene
     cd /cluster/data/metAce1/bed/loweTrnaGene
     hgLoadBed -tab metAce1 loweTrnaGene metAce1-lowe-trnas.bed -sqlTable=~/kent/src/hg/lib/loweTrnaGene.sql
 
 # TODD LOWE snoRNA GENES ()
     # This is a bed 6 file created by hand.
     ssh hgwdev
     mkdir /cluster/data/metAce1/bed/loweSnoGene
     cd /cluster/data/metAce1/bed/loweSnoGene
     hgLoadBed -tab metAce1 loweSnoGene metAce1-snos.bed
 
 # TIGR GENES (DONE)
     # First go to http://www.tigr.org/tigr-scripts/CMR2/gene_attribute_form.dbi
     # and fill out the web form as follows:
     #   - Pick "Retrieve attributes for the specified DNA feature within a specific 
     #     organism and/or a specific role category".
     #       * Pick "Pyrobaculum aerophilum IM2", and "Primary and TIGR annotation ORFs" 
     #         from the 1st and 3rd box.
     #       * Select everything from "Choose TIGR Annotation Gene Attributes"
     #       * Select "Primary Locus Name" from "Choose Primary Annotation Gene Attributes"
     #       * Select everything from "Choose Other Gene Attributes"
     #   - Click submit, and click save as tab-delimited file.
     ssh hgwdev
     mkdir /cluster/data/metAce1/bed/tigrCmrORFs
     cp metAce1-tigr.tab /cluster/data/metAce1/bed/tigrCmrORFs
     cd /cluster/data/metAce1/bed/tigrCmrORFs
     /projects/lowelab/users/aamp/bin/i386/tigrCmrToBed metAce1-tigr.tab metAce1-tigr.bed
     hgLoadBed -tab metAce1 tigrCmrORFs metAce1-tigr.bed -sqlTable=~/kent/src/hg/lib/tigrCmrGene.sql
 
 ###########################################################
 #mulitz pHMM conservation track for 3 Methosarcina species#
 ###########################################################
 ## DONE: 8/14/05 (baertsch)
 
 cd /cluster/data/metAce1/bed
 mkdir conservation
 cd conservation
 cp /cluster/data/pyrFur2/bed/conservation/HoxD55.q .
 
 #get fastas
 cp ../../chr.fa metAce1.chr
 cp /projects/lowelab/db/blastdb/Meth_maze.fa metMaz1.fa
 wget ftp://ftp.jgi-psf.org/pub/JGI_data/Microbial/methanosarcina/031109/2351479_fasta.screen.contigs
 mv 2351479_fasta.screen.contigs metBak0.chr
 ftp://ftp.jgi-psf.org/pub/JGI_data/Microbial/methanococcoides_burtonii/041112/2662199.fsa
 mv 2662199.fsa metBur0.chr
 #edit > lines to say metAce1.chr, metBak0.contigN, and metMaz1.chr
 
 #make nibs and 2bits
 /cluster/bin/i386/faToNib metAce1.chr metAce1.chr.nib
 /cluster/bin/i386/faToNib metMaz1.chr metMaz1.chr.nib
 /cluster/bin/i386/faToTwoBit metBak0.chr metBak0.2bit
 /cluster/bin/i386/faToTwoBit metBur0.chr metBur0.2bit
 
 
 #chrom sizes
 faSize -detailed *.chr > chrom.sizes
 
 #blastz
 blastz metAce1.chr metBak0.chr Q=HoxD55.q > metAce1-metBak0.lav
 blastz metAce1.chr metMaz1.chr Q=HoxD55.q > metAce1-metMaz1.lav
 blastz metAce1.chr metBur0.chr Q=HoxD55.q > metAce1-metBur0.lav
 /cluster/bin/i386/lavToAxt metAce1-metBak0.lav . metBak0.2bit metAce1-metBak0.axt
 /cluster/bin/i386/lavToAxt metAce1-metMaz1.lav . . metAce1-metMaz1.axt
 /cluster/bin/i386/lavToAxt metAce1-metBur0.lav . metBur0.2bit metAce1-metBur0.axt
 
 axtBest metAce1-metBak0.axt metAce1.chr -winSize=500 -minScore=5000 metAce1-metBak0-best.axt
 axtBest metAce1-metMaz1.axt metAce1.chr -winSize=500 -minScore=5000 metAce1-metMaz1-best.axt
 axtBest metAce1-metBur0.axt metAce1.chr -winSize=500 -minScore=5000 metAce1-metBur0-best.axt
 axtToMaf metAce1-metBak0-best.axt chrom.sizes chrom.sizes metAce1-metBak0.maf
 axtToMaf metAce1-metMaz1-best.axt chrom.sizes chrom.sizes metAce1-metMaz1.maf
 axtToMaf metAce1-metBur0-best.axt chrom.sizes chrom.sizes metAce1-metBur0.maf
 
 #multiz: v10 has new parameter (3rd par), 0=2 seqs not align to ref, else 1.
 # stick with v8 for now.
 multiz metAce1-metBak0.maf metAce1-metMaz1.maf - > metAce1-metBak0-metMaz1.maf
 
 
 #phyloHMM
 /cluster/bin/phast/msa_view -i MAF -M metAce1.chr -o SS metAce1-metBak0-metMaz1.maf > metAce1.ss
 /cluster/bin/phast/phyloFit -i SS metAce1.ss -t "(metAce1,(metBak0,metMaz1))" 
 /cluster/bin/phast/msa_view -i SS metAce1.ss --summary-only
 #descrip.                      A          C          G          T        G+C     length   all_gaps  some_gaps
 #metAce1.ss               0.2892     0.2088     0.2089     0.2931     0.4177    6057454          0     580625
 /cluster/bin/phast/phastCons metAce1.ss phyloFit.mod --gc 0.4177 \
  --target-coverage 0.5 --estimate-trees met-tree \
  --expected-lengths 7 --no-post-probs --ignore-missing \
  --nrates 1,1
 /cluster/bin/phast/phastCons metAce1.ss \
  met-tree.cons.mod,met-tree.noncons.mod \
  --target-coverage 0.5 --expected-lengths 75 \
  --viterbi metAce-elements.bed --score \
  --require-informative 0 --seqname chr > cons.dat
 wigEncode cons.dat phastCons.wig phastCons.wib
 
 #phlyloHmmm new
 
 multiz metAce1-metMaz1.maf metAce1-metBak0.maf - > metAce1-metMaz1-metBak0.maf
 /cluster/bin/phast/msa_view -i MAF -M metAce1.chr -o SS metAce1-metMaz1-metBak0.maf > metAce1new.ss
 /cluster/bin/phast/phyloFit -i SS metAce1new.ss -t "(metBak0,(metAce1,metMaz1))" -o Ma1Mz1Mb0
 /cluster/bin/phast/msa_view -i SS metAce1new.ss --summary-only 
 #descrip.                      A          C          G          T        G+C     length   all_gaps  some_gaps
 #metAce1new.ss            0.2892     0.2088     0.2090     0.2930     0.4178    6057224          0     579124
 /cluster/bin/phast/phastCons metAce1new.ss Ma1Mz1Mb0.mod --gc 0.4177 \
  --target-coverage 0.7 --estimate-trees met-tree2 \
  --expected-lengths 7 --no-post-probs --ignore-missing \
  --nrates 1,1
 /cluster/bin/phast/phastCons metAce1new.ss \
  met-tree2.cons.mod,met-tree2.noncons.mod \
  --target-coverage 0.7 --expected-lengths 75 \
  --viterbi metAce-elements2.bed --score \
  --require-informative 0 --seqname chr > cons2.dat
 wigEncode cons2.dat phastCons2.wig phastCons2.wib
 
 
 #move data
 mkdir wib
 mv phastCons.wib wib/phastCons.wib
 mv phastCons.wig wib/phastCons.wig
 ln -s /cluster/data/metAce1/bed/conservation/wib/phastCons.wib /gbdb/metAce1/wib
 mkdir /gbdb/metAce1/pwMaf
 mkdir -p otherMet/metBak0 otherMet/metMaz1
 mv metAce1-metBak0.maf otherMet/metBak0/chr.maf
 mv metAce1-metMaz1.maf otherMet/metMaz1/chr.maf
 ln -s /cluster/data/metAce1/bed/conservation/otherMet/metBak0 /gbdb/metAce1/pwMaf/metBak0_pwMaf
 ln -s /cluster/data/metAce1/bed/conservation/otherMet/metMaz1 /gbdb/metAce1/pwMaf/metMaz1_pwMaf
 mkdir multiz
 mv metAce1-metBak0-metMaz1.maf multiz/chr.maf
 ln -s /cluster/data/metAce1/bed/conservation/multiz /gbdb/metAce1/multizMa1Mb1Mz1
 
 #load
 hgLoadWiggle metAce1 phastCons /cluster/data/metAce1/bed/conservation/wib/phastCons.wig
 hgLoadMaf -warn metAce1 multizMa1Mb1Mz1
 hgLoadMaf -warn metAce1 metBak0_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metBak0_pwMaf
 hgLoadMaf -warn metAce1 metMaz1_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metMaz1_pwMaf
 
 #trackDb.ra entry
  # track multizMa1Mb1Mz1
  # shortLabel Conservation
  # longLabel Methosarcina  3-way multiz alignments
  # group compGeno
  # priority 10.0
  # visibility pack
  # type wigMaf 0.0 1.0
  # maxHeightPixels 100:40:11
  # wiggle phastCons
  # yLineOnOff Off
- # autoScaleDefault Off
+ # autoScale Off
  # pairwise pwMaf
  # speciesOrder metBak0 metMaz1
 cd ~/kent/src/hg/makeDb/trackDb/archae/metAce1
 cvs commit -m "Added multiz track" trackDb.ra
 
 #html page for multizMa1Mb1Mz1
 cd ~/kent/src/hg/makeDb/trackDb/archae/metAce1
 cvs add multizMa1Mb1Mz1.html
 cvs commit -m "Details page for multiz track" multizMa1Mb1Mz1.html
 
 ## 4 way
 multiz metAce1-metBur0.maf metAce1-metMaz1-metBak0.maf - > metAce1-metMaz1-metBak0-metBur0.maf
 /cluster/bin/phast/msa_view -i MAF -M metAce1.chr -o SS metAce1-metMaz1-metBak0-metBur0.maf > metAce4way.ss
 /cluster/bin/phast/phyloFit -i SS metAce4way.ss -t "(metBur0,(metBak0,(metAce1,metMaz1)))" -o Ma1Mz1Mbk0Mbr0
 /cluster/bin/phast/msa_view -i SS metAce4way.ss --summary-only 
 #descrip.                      A          C          G          T        G+C     length   all_gaps  some_gaps
 #metAce4way.ss            0.2887     0.2092     0.2094     0.2926     0.4186    6100179          0     710386
 /cluster/bin/phast/phastCons metAce4way.ss Ma1Mz1Mbk0Mbr0.mod --gc 0.4186 \
  --target-coverage 0.7 --estimate-trees met-tree4 \
  --expected-lengths 7 --no-post-probs --ignore-missing \
  --nrates 1,1
 /cluster/bin/phast/phastCons metAce4way.ss \
  met-tree4.cons.mod,met-tree4.noncons.mod \
  --target-coverage 0.7 --expected-lengths 75 \
  --viterbi metAce-elements2.bed --score \
  --require-informative 0 --seqname chr > cons4.dat
 wigEncode cons4.dat phastCons4.wig phastCons4.wib
 
 draw_tree Ma1Mz1Mbk0Mbr0.mod  > tree4.ps
 ps2pdf tree4.ps tree4.pdf
 #move data
 mv phastCons4.wib wib/phastCons4.wib
 mv phastCons4.wig wib/phastCons4.wig
 ln -s /cluster/data/metAce1/bed/conservation/wib/phastCons4.wib /gbdb/metAce1/wib
 mkdir -p otherMet/metBur0 
 mv metAce1-metBur0.maf otherMet/metBur0/chr.maf
 ln -s /cluster/data/metAce1/bed/conservation/otherMet/metBur0 /gbdb/metAce1/pwMaf/metBur0_pwMaf
 mkdir multiz4way
 mv metAce1-metMaz1-metBak0-metBur0.maf multiz4way/chr.maf
 ln -s /cluster/data/metAce1/bed/conservation/multiz4way /gbdb/metAce1/multizMa1Mz1Mbk0Mbr0
 
 #load
 hgLoadWiggle metAce1 phastCons4 /cluster/data/metAce1/bed/conservation/wib/phastCons4.wig
 hgLoadMaf -warn metAce1 multizMa1Mz1Mbk0Mbr0
 hgLoadMaf -warn metAce1 metBur0_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metBur0_pwMaf
    
 #trackDb.ra entry
  # track multizMa1Mz1Mbk0Mbr0
  # shortLabel Cons 4way
  # longLabel Methosarcina Acetivorans/Mazei/Bakeri 3-way multiz alignments + Methanobacteriodes Burtonii
  # group compGeno
  # priority 10.0
  # visibility pack
  # type wigMaf 0.0 1.0
  # maxHeightPixels 100:40:11
  # wiggle phastCons
  # yLineOnOff Off
- # autoScaleDefault Off
+ # autoScale Off
  # pairwise pwMaf
  # speciesOrder metMaz1 metBak0 metBur0
 
 
 ## re-run with 25 expected length parameter
 /cluster/bin/phast/phastCons metAce4way.ss Ma1Mz1Mbk0Mbr0.mod --gc 0.4186 \
  --target-coverage 0.7 --estimate-trees met-tree4.25 \
  --expected-lengths 25 --no-post-probs --ignore-missing \
  --nrates 1,1
 /cluster/bin/phast/phastCons metAce4way.ss \
  met-tree4.25.cons.mod,met-tree4.25.noncons.25.mod \
  --target-coverage 0.7 --expected-lengths 25 \
  --viterbi metAce-elements.25.bed --score \
  --require-informative 0 --seqname chr > cons4.25.dat
 
 wigEncode cons4.25.dat phastCons4.25.wig phastCons4.25.wib
 mkdir wib
 mv phastCons4.25.wib wib/phastCons4.25.wib
 mv phastCons4.25.wig wib/phastCons4.25.wig
 ln -s /cluster/data/metAce1/bed/conservation/wib/phastCons.wib /gbdb/metAce1/wib
 hgLoadWiggle metAce1 phastCons4_25 /cluster/data/metAce1/bed/conservation/phastCons4.25.wig
 
 # 3 states, 2 conserved , 1 nonconserved
 nice /cluster/bin/phast/phastCons metAce4way.ss Ma1Mz1Mbk0Mbr0.mod --gc 0.4186 \
  --target-coverage 0.7 --estimate-trees met-tree4.25.3state  \
  --expected-lengths 25 --no-post-probs --ignore-missing  \
  --nrates 2,1 
 
 /cluster/bin/phast/phastCons metAce4way.ss \
  met-tree4.25.cons1.mod,met-tree4.25.cons2.mod,met-tree4.25.3state.noncons.25.mod \
  --target-coverage 0.7 --expected-lengths 25 \
  --viterbi metAce-elements.3state.25.bed --score \
  --require-informative 0 --seqname chr > cons4.25.3state.dat
 
 #FINISH UP MULTIZ TRACK (4way) 
 # DONE (10/9/05), kpollard
 
     cd /cluster/data/metAce1/bed/conservation/
     #move data
     cp tree4.jpg /usr/local/apache/htdocs/images/metAce1-tree.jpg
     cd wib
     mv phastCons.wib phastCons3.wib
     mv phastCons.wig phastCons3.wig
     cp phastCons4.25.wib phastCons.wib
     cp phastCons4.25.wig phastCons.wig
     ln -s /cluster/data/metAce1/bed/conservation/wib/phastCons.wib /gbdb/metAce1/wib
     #drop tables so can reload with new data
     hgLoadWiggle metAce1 phastCons /cluster/data/metAce1/bed/conservation/wib/phastCons.wig
     hgLoadMaf -warn metAce1 multizMa1Mz1Mbk0Mbr0
     hgLoadMaf -warn metAce1 metBak0_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metBak0_pwMaf
     hgLoadMaf -warn metAce1 metBur0_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metBur0_pwMaf
     hgLoadMaf -warn metAce1 metMaz1_pwMaf -pathPrefix=/gbdb/metAce1/pwMaf/metMaz1_pwMaf
     hgLoadBed metAce1 phastConsElements metAce-elements.25.bed 
     #trackDb
     cd ~/kent/src/hg/makeDb/trackDb/archae/metAce1
     #trackDb.ra entry
     # track multizMa1Mz1Mbk0Mbr0
     # shortLabel Cons 4way
     # longLabel Methosarcina 4-way multiz alignments
     # group compGeno
     # priority 10.0
     # visibility pack
     # type wigMaf 0.0 1.0
     # maxHeightPixels 100:40:11
     # wiggle phastCons
     # yLineOnOff Off
-    # autoScaleDefault Off
+    # autoScale Off
     # pairwise pwMaf
     # speciesOrder metMaz1 metBak0 metBur0
     cvs commit -m "New multiz track" trackDb.ra
     #html page
     mv multizMa1Mb1Mz1.html multizMa1Mz1Mbk0Mbr0.html
     cvs remove multizMa1Mb1Mz1.html
     cvs add multizMa1Mz1Mbk0Mbr0.html
     cvs commit -m "Details page for multiz track" multizMa1Mz1Mbk0Mbr0.html