src/hg/makeDb/doc/hg19.txt 1.84
1.84 2010/02/18 20:29:50 hartera
Added a Signal track for the Burge RNA-seq data. Updated trackDb entry for this track to include views.
Index: src/hg/makeDb/doc/hg19.txt
===================================================================
RCS file: /projects/compbio/cvsroot/kent/src/hg/makeDb/doc/hg19.txt,v
retrieving revision 1.83
retrieving revision 1.84
diff -b -B -U 4 -r1.83 -r1.84
--- src/hg/makeDb/doc/hg19.txt 18 Feb 2010 18:53:25 -0000 1.83
+++ src/hg/makeDb/doc/hg19.txt 18 Feb 2010 20:29:50 -0000 1.84
@@ -7276,8 +7276,15 @@
# 2009-12-14, hartera. Set cdsStart = cdsEnd = 0. Moved track data directory to
# /hive/data/genomes/hg18/bed.
# 2010-01-04, hartera. Change the data to BED format and re-loaded tables. BED
# is more appropriate for this data type.
+# The data is too dense in places (feedback from QA) so it would be more
+# appropriate to have a Signal track as for the ENCODE RNA-seq data tracks.
+# 2010-02-09, hartera. Create bedGraph Signal subtracks for each tissue/cell
+# using reads/per million mapped reads as the data value.
+# 2010-02-17, hartera. Updated trackDb.ra entry to include views.
+# 2010-02-18, hartera. Loaded the bedGraph tables for the Raw Signal
+# subtracks.
mkdir /hive/groups/gencode/browser/hg19/burgeRnaSeqGemMapperAlign
cd /hive/groups/gencode/browser/hg19/burgeRnaSeqGemMapperAlign
@@ -7375,8 +7382,31 @@
end
# Changed track type in trackDb/human/trackDb.ra to bed 12 and
# then did make alpha in trackDb directory.
+ # 2010-02-09. Add a Signal track so it is easier to view the data in
+ # regions where there is a high density of reads.
+ cd /hive/data/genomes/hg19/bed/burgeRnaSeqGemMapperAlign
+ # Use bedItemOverlapCount to get counts of overlapping items for each base.
+ # Need to sort the bed files and then get the number of reads mapped for
+ # that tissue. Divide the counts by the number of million mapped reads to
+ # get the number of reads per million mapped reads as the data value.
+ foreach f (`ls *.bed`)
+ echo $f
+ set g=$f:r
+ sort $f | bedItemOverlapCount hg19 stdin > ${f}.count
+ set size=`hgsql -Ne "select count(distinct name) from ${g};" hg19`
+ awk -v size=${size} 'BEGIN {OFS="\t"} {print $1,$2,$3,($4 / (size/1000000));}' ${f}.count > ${g}.bedGraph
+ end
+ # (2010-02-18, hartera)
+ # Load the bedGraph tables into the database as Raw Signal tracks.
+ foreach f (`ls *.bedGraph`)
+ echo $f
+ set g=$f:r
+ hgLoadBed -bedGraph=4 hg19 ${g}AllRawSignal $f >>& loadSignal.log
+ end
+ # trackDb/human/trackDb.ra entry was updated to include views for Raw Signal
+ # and Alignment subtracks (2010-02-17)
##########################################################################
# BUILD ALLEN BRAIN TRACK (DONE 09/30/09 kent)
# Make the working directory
@@ -8591,12 +8621,4 @@
cat fb.calJac3.chainHg19Link.txt
# 2030475813 bases of 2752505800 (73.768%) in intersection
#####################################################################
-# PAR track (2010-02-18, markd DONE)
-
- cd /hive/data/genomes/hg19/bed/par/
- # create hg19.par using the documented coordinates
- hgPar hg19 hg19.par par
-#####################################################################
-
-