src/hg/makeDb/trackDb/affyExonTissues.html 1.7
1.7 2010/03/12 00:41:40 pauline
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<H2>Methods</H2>
<P>
RNA (from a commercial source) from 11 tissues were hybridized to Affymetrix
$Organism Exon 1.0 ST arrays. For each tissue, 3 replicate experiments were
performed
for a total of 33 arrays. The raw intensity signal from the arrays
was normalized
with a quantile normalization method, then run through the PLIER algorithm.
The normalized data were then converted to median-centered log-ratios,
which are displayed as green for negative log-ratios (below-median expression),
and red for positive (above-median expression).</P>
<P>The probe sets for this microarray track are shown in the
the <B>Affy Exon Probes</B> track.</P>
<H2>Credits</H2>
<P>
This track was produced by Andy Pohl, Kayla Smith, and Pauline Fujita of the
genome browser group at UCSC, Melissa Cline of the
<A HREF="http://ribonode.ucsc.edu/" TARGET=_blank>Ares lab</A> at UCSC, and
Chuck Sugnet at <A HREF="http://www.affymetrix.com/" TARGET=_blank>Affymetrix</A>, based on
<A HREF="http://www.affymetrix.com/support/technical/sample_data/exon_array_data.affx">
sample exon array data</A> available from Affymetrix, produced by Tyson Clark.
</P>
<H2>References</H2>
<P>
Pohl AA, Sugnet CW, Clark TA, Smith K, Fujita PA, Cline MS.
Affy Exon Tissues: Exon Levels in Normal Tissues in Human, Mouse, and Rat.
-<B>Bioinformatics</B>. In press.
+<I>Bioinformatics</I>.2009 Sept 15;25(18):2442-3.
</P>
<H2>Links</H2>
<P><UL><LI>
<A HREF="http://www.affymetrix.com/products/arrays/specific/exon.affx">Affymetrix $Organism Exon 1.0 ST array web page</A></LI>
<LI><A HREF="http://www.affymetrix.com/support/technical/sample_data/exon_array_data.affx">Affymetrix $Organism Exon data</A></LI>
<LI><A HREF="http://www.affymetrix.com/support/technical/technotes/plier_technote.pdf">PLIER algorithm documentation (PDF).</A></LI></UL></P>