e70152e44cc66cc599ff6b699eb8adc07f3e656a kent Sat May 24 21:09:34 2014 -0700 Adding Copyright NNNN Regents of the University of California to all files I believe with reasonable certainty were developed under UCSC employ or as part of Genome Browser copyright assignment. diff --git src/utils/cpgIslandExt/cpg.c src/utils/cpgIslandExt/cpg.c index 0afb018..52b6215 100644 --- src/utils/cpgIslandExt/cpg.c +++ src/utils/cpgIslandExt/cpg.c @@ -1,174 +1,177 @@ +/* Copyright (C) 2012 The Regents of the University of California + * See README in this or parent directory for licensing information. */ + /* Last edited: Jun 23 19:33 1995 (gos) */ /* cpg.c: CpG island finder. Larsen et al Genomics 13 1992 p1095-1107 "usually defined as >200bp with %GC > 50% and obs/exp CpG > 0.6". Here use running sum rather than window to score: if not CpG at postion i, then decrement runSum counter, but if CpG then runSum += CPGSCORE. Spans > threshold are searched for recursively. filename: fasta format concatenated sequence file: */ /********************************************************* put in options: -p debug to switch on commented out print statements -l length threshold -t score threshold -s to specify score -d score (-ve) if not found : default = -1. Really want it to take pattern file: Pattern score_if_found score_if_not_found How best to give #pattern hits, %GC in span, o/e ? Cleanest: Provide using separate scripts called using output. Add -g or -m option for "max gap" as in Qpatch.c - score not increased if reaches this threshold. This means that patches which are separated by more than the threshold are never joined. *********************************************************/ #include "stdio.h" #include "math.h" #include "readseq.h" /********************* CONTROLS ********************/ #define CPGSCORE 17 /* so that can compare with old cpg - this had CPGSCORE 27, but allowed score to reach 0 once without reporting */ #define MALLOCBLOCK 200000 #define MAXNAMELEN 512 /*"length 0, title > MAXNAMELEN or starts with bad character." if too small */ /*------------------------------------------------------*/ int readSequence (FILE *fil, int *conv, char **seq, char **id, char **desc, int *length) ; int findspans ( int start, int end, char *seq, char *seqname ) ; void getstats ( int start, int end, char *seq, char *seqname, int *ncpg, int *ngpc, int *ngc ) ; void usage (void) { fprintf (stderr, "Usage: cpg seqfilename\n") ; exit (-1) ; } /*------------------------------------------------------*/ void main (int argc, char **argv) { char *seq, *seqname, *desc ; int conv[] = { 0, 1, 2, 3, 4 } ; int length ; int i ; static FILE *fil ; char c, *cp ; extern char* malloc() ; /*------------------------------------------------------*/ switch ( argc ) { default: if (argc != 2) usage () ; } if (!(seqname = malloc (MAXNAMELEN+1))) { fprintf (stderr, "Couldn't malloc %d bytes", MAXNAMELEN) ; exit (-1) ; } if (!(seq = malloc (MALLOCBLOCK+1))) { fprintf (stderr, "Couldn't malloc %d bytes", MALLOCBLOCK) ; exit (-1) ; } if (!(fil = fopen ( argv[1], "r" ))) usage (); while ( readSequence(fil, dna2textConv, &seq, &seqname, &desc, &length) ) /* once through per sequence */ { i = 0 ; while ( seqname[i] != ' ' && seqname[i] != '\0' && i < 256 ) ++i ; seqname[i] = '\0' ; findspans ( 0, length, seq, seqname ) ; } exit (0); } int findspans ( int start, int end, char *seq, char *seqname ) { int i ; int sc = 0 ; int lsc = 0 ; int imn = -1 ; /* else sequences which start with pattern reported badly */ int imx = 0 ; int mx = 0 ; int ncpg, ngpc, ngc ; i = start ; while ( i < end ) { lsc = sc ; sc = ( end-1-i && seq[i]=='C' && seq[i+1]=='G' ) ? sc += CPGSCORE : --sc ; sc = sc < 0 ? 0 : sc ; /* printf("%d \t %d \t%d \t %d \t%d \t%d\n", i, sc, lsc, imn, imx, mx) ; */ if ( sc == 0 && lsc ) /* should threshold here */ { /* imn+1 is the start of the match. imx+1 is the end of the match, given pattern-length=2. fetchdb using reported co-ordinates will return a sequence which both starts and ends with a complete pattern. Further +1 adjusts so that start of sequence = 1 */ getstats ( imn+1, imx+2, seq, seqname, &ncpg, &ngpc, &ngc ) ; printf("%s\t %d\t %d\t %d\t CpG: %d\t %.1f\t %.1f\n", seqname, imn+2, imx+2, mx, ncpg, ngc*100.0/(imx+1-imn), 1.0*ncpg/ngpc) ; /* printf("%s \t %d\t %d\t %d \n", seqname, imn+2, imx+2, mx ) ; */ /* Recursive call searches from one position after the end of the last match to the current position */ findspans( imx+2, i, seq, seqname ) ; sc = lsc = imn = imx = mx = 0 ; } imx = sc > mx ? i : imx ; mx = sc > mx ? sc : mx ; imn = sc == 0 ? i : imn ; ++i ; } if ( sc != 0 ) /* should threshold here */ { /* printf("%d \t %d \t%d \t %d \t%d \t%d\n", i, sc, lsc, imn, imx, mx) ; */ getstats ( imn+1, imx+2, seq, seqname, &ncpg, &ngpc, &ngc ) ; printf("%s\t %d\t %d\t %d\t CpG: %d\t %.1f\t %.2f\n", seqname, imn+2, imx+2, mx, ncpg, ngc*100.0/(imx+1-imn), 1.0*ncpg/ngpc) ; /* printf("%s \t %d\t %d\t %d \n", seqname, imn+2, imx+2, mx ) ; */ findspans( imx+2, end, seq, seqname ) ; } } void getstats ( int start, int end, char *seq, char *seqname, int *ncpg, int *ngpc, int *ngc ) { int i ; *ncpg = *ngpc = *ngc = 0 ; for ( i = start ; i < end ; ++i ) { if ( end-1-i && seq[i]=='C' && seq[i+1]=='G' ) ++*ncpg ; if ( end-1-i && seq[i]=='G' && seq[i+1]=='C' ) ++*ngpc ; if ( seq[i]=='C' || seq[i]=='G' ) ++*ngc ; } }