1d76bf881d3e18580739e44698a0d1a499c2f9f1 hiram Fri Oct 2 10:31:39 2015 -0700 fixup gcc warnings for -Wunused-but-set-variable refs #16121 diff --git src/lib/pslGenoShow.c src/lib/pslGenoShow.c index 2e3e46d..507a9e2 100644 --- src/lib/pslGenoShow.c +++ src/lib/pslGenoShow.c @@ -1,349 +1,341 @@ /* Show aligned exons between a pre-located gene (a stamper gene) in the genome *and its homologues (stamp elements) in the genome. *The aligned exon sequences are shown in blue as regular blat alignment. * The unaligned exon sequence are shown in red. Intron sequences are shown in black. * It is modified from pslShow.c */ /* Copyright (C) 2011 The Regents of the University of California * See README in this or parent directory for licensing information. */ #include "common.h" #include "dnaseq.h" #include "htmshell.h" #include "psl.h" #include "cda.h" #include "seqOut.h" static void pslShowAlignmentStranded2(struct psl *psl, boolean isProt, char *qName, bioSeq *qSeq, int qStart, int qEnd, char *tName, bioSeq *tSeq, int tStart, int tEnd, int exnStarts[], int exnEnds[], int exnCnt, FILE *f) /* Show stamper gene and stamp elements alignment using genomic sequence. * The aligned exons' sequence of stamper gene are shown in colors as usual, but the * the unaligned exon's sequence of stamper gene are shown in red color. */ { boolean tIsRc = (psl->strand[1] == '-'); boolean qIsRc = (psl->strand[0] == '-'); int mulFactor = (isProt ? 3 : 1); DNA *dna = NULL; /* Mixed case version of genomic DNA. */ int qSize = qSeq->size; char *qLetters = cloneString(qSeq->dna); -int qbafStart, qbafEnd, tbafStart, tbafEnd; -int qcfmStart, qcfmEnd, tcfmStart, tcfmEnd; +int qbafStart, tbafStart; +int qcfmStart, tcfmStart; int lineWidth = isProt ? 60 : 50; tbafStart = tStart; -tbafEnd = tEnd; tcfmStart = tStart; -tcfmEnd = tEnd; qbafStart = qStart; -qbafEnd = qEnd; qcfmStart = qStart; -qcfmEnd = qEnd; /* Deal with minus strand. */ if (tIsRc) { int temp; reverseComplement(tSeq->dna, tSeq->size); tbafStart = tEnd; - tbafEnd = tStart; tcfmStart = tEnd; - tcfmEnd = tStart; temp = psl->tSize - tEnd; tEnd = psl->tSize - tStart; tStart = temp; } if (qIsRc) { int temp, j; reverseComplement(qSeq->dna, qSeq->size); reverseComplement(qLetters, qSeq->size); qcfmStart = qEnd; - qcfmEnd = qStart; qbafStart = qEnd; - qbafEnd = qStart; temp = psl->qSize - qEnd; qEnd = psl->qSize - qStart; qStart = temp; for(j = 0; j < exnCnt; j++) { temp = psl->qSize - exnStarts[j]; exnStarts[j] = psl->qSize - exnEnds[j]; exnEnds[j] = temp; } reverseInts(exnEnds, exnCnt); reverseInts(exnStarts, exnCnt); } dna = cloneString(tSeq->dna); if (qName == NULL) qName = psl->qName; if (tName == NULL) tName = psl->tName; fputs("Matching bases are colored blue and capitalized. " "Light blue bases mark the boundaries of gaps in either aligned sequence. " "Red bases are unaligned exons' bases of the query gene. \n", f); fprintf(f, "

%s%s

\n", qName, (qIsRc ? " (reverse complemented)" : "")); fprintf(f, "
");
 tolowers(qLetters);
 
 /* Display query sequence. */
     {
     struct cfm *cfm;
     char *colorFlags = needMem(qSeq->size);
     int i = 0, j = 0, exnIdx = 0;
     int preStop = 0;
     
     for (i=0; iblockCount; ++i)
 	{
 	int qs = psl->qStarts[i] - qStart;
 	int ts = psl->tStarts[i] - tStart;
 	int sz = psl->blockSizes[i]-1;
 	int end = 0;
 	bool omitExon = FALSE;
 	while(exnIdx < exnCnt && psl->qStarts[i] > exnEnds[exnIdx])
 	    {
 	    if(omitExon)
 		{
 		for( j = exnStarts[exnIdx] - qStart; j < exnEnds[exnIdx]-qStart; j++)
 		    {
 		    colorFlags[j] = socRed;
 		    }
 		}
 	    exnIdx++;
 	    preStop = exnStarts[exnIdx] - qStart;
 	    omitExon = TRUE;
 	    }
 
 	/*mark the boundary bases */
 	colorFlags[qs] = socBrightBlue;
 	qLetters[qs] = toupper(qLetters[qs]);
 	colorFlags[qs+sz] = socBrightBlue;
 	qLetters[qs+sz] = toupper(qLetters[qs+sz]);
 	
 	/* determine block end */
 	if( i < psl->blockCount -1)
 	    end = psl->qStarts[i+1] < exnEnds[exnIdx] ? psl->qStarts[i+1] - qStart : exnEnds[exnIdx] - qStart;
 	else
 	    end = qs + sz;
 	    
 	for (j=preStop; j < end; j++)
 	    {
 	    if(j == 82)
 		fprintf(stderr, "right here\n");
 	    if (j > qs && j < qs+sz)
 		{
 		if (qSeq->dna[j] == tSeq->dna[ts+j-qs])
 		    {
 		    colorFlags[j] = socBlue;
 		    qLetters[j] = toupper(qLetters[j]);
 		    }		
 		}
 	    else if(colorFlags[j] != socBrightBlue && colorFlags[j] != socBlue)
 		colorFlags[j] = socRed;
 	    }
 	preStop = end;
 	}
     cfm = cfmNew(10, lineWidth, TRUE, qIsRc, f, qcfmStart);
     for (i=0; i
\n"); fprintf(f, "

%s %s:

\n", tName, (tIsRc ? "(reverse strand)" : "")); fprintf(f, "
");
 
 /* Display DNA sequence. */
     {
     struct cfm *cfm;
     char *colorFlags = needMem(tSeq->size);
     int i,j;
     int curBlock = 0;
 
     for (i=0; iblockCount; ++i)
 	{
 	int qs = psl->qStarts[i] - qStart;
 	int ts = psl->tStarts[i] - tStart;
 	int sz = psl->blockSizes[i];
 	if (isProt)
 	    {
 	    for (j=0; jdna[qs+j];
 		int codonStart = ts + 3*j;
 		DNA *codon = &tSeq->dna[codonStart];
 		AA trans = lookupCodon(codon);
 		if (trans != 'X' && trans == aa)
 		    {
 		    colorFlags[codonStart] = socBlue;
 		    colorFlags[codonStart+1] = socBlue;
 		    colorFlags[codonStart+2] = socBlue;
 		    toUpperN(dna+codonStart, 3);
 		    }
 		}
 	    }
 	else
 	    {
 	    for (j=0; jdna[qs+j] == tSeq->dna[ts+j])
 		    {
 		    colorFlags[ts+j] = socBlue;
 		    dna[ts+j] = toupper(dna[ts+j]);
 		    }
 		}
 	    }
 	colorFlags[ts] = socBrightBlue;
 	colorFlags[ts+sz*mulFactor-1] = socBrightBlue;
 	}
 
     cfm = cfmNew(10, lineWidth, TRUE, tIsRc, f, tcfmStart);
 	
     for (i=0; isize; ++i)
 	{
 	/* Put down "anchor" on first match position in haystack
 	 * so user can hop here with a click on the needle. */
 	if (curBlock < psl->blockCount && psl->tStarts[curBlock] == (i + tStart) )
 	    {
 	    fprintf(f, "", ++curBlock);
 	    /* Watch out for (rare) out-of-order tStarts! */
 	    while (curBlock < psl->blockCount &&
 		   psl->tStarts[curBlock] <= tStart + i)
 		curBlock++;
 	    }
 	cfmOut(cfm, dna[i], seqOutColorLookup[(int)colorFlags[i]]);
 	}
     cfmFree(&cfm);
     freez(&colorFlags);
     htmHorizontalLine(f);
     }
 
 /* Display side by side. */
 fprintf(f, "
\n"); fprintf(f, "

Side by Side Alignment*

\n"); fprintf(f, "
");
     {
     struct baf baf;
     int i,j;
 
     bafInit(&baf, qSeq->dna, qbafStart, qIsRc,
 	    tSeq->dna, tbafStart, tIsRc, f, lineWidth, isProt);
 		
     if (isProt)
 	{
 	for (i=0; iblockCount; ++i)
 	    {
 	    int qs = psl->qStarts[i] - qStart;
 	    int ts = psl->tStarts[i] - tStart;
 	    int sz = psl->blockSizes[i];
 
 	    bafSetPos(&baf, qs, ts);
 	    bafStartLine(&baf);
 	    for (j=0; jdna[qs+j];
 		int codonStart = ts + 3*j;
 		DNA *codon = &tSeq->dna[codonStart];
 		bafOut(&baf, ' ', codon[0]);
 		bafOut(&baf, aa, codon[1]);
 		bafOut(&baf, ' ', codon[2]);
 		}
 	    bafFlushLine(&baf);
 	    }
 	fprintf( f, "*when aa is different, BLOSUM positives are in green, BLOSUM negatives in red\n");
 	}
     else
 	{
 	int lastQe = psl->qStarts[0] - qStart;
 	int lastTe = psl->tStarts[0] - tStart;
 	int maxSkip = 8;
 	bafSetPos(&baf, lastQe, lastTe);
 	bafStartLine(&baf);
 	for (i=0; iblockCount; ++i)
 	    {
 	    int qs = psl->qStarts[i] - qStart;
 	    int ts = psl->tStarts[i] - tStart;
 	    int sz = psl->blockSizes[i];
 	    boolean doBreak = TRUE;
 	    int qSkip = qs - lastQe;
 	    int tSkip = ts - lastTe;
 
 	    if (qSkip >= 0 && qSkip <= maxSkip && tSkip == 0)
 		{
 		for (j=0; jdna[lastQe+j], '-');
 		doBreak = FALSE;
 		}
 	    else if (tSkip > 0 && tSkip <= maxSkip && qSkip == 0)
 		{
 		for (j=0; jdna[lastTe+j]);
 		doBreak = FALSE;
 		}
 	    if (doBreak)
 		{
 		bafFlushLine(&baf);
 		bafSetPos(&baf, qs, ts);
 		bafStartLine(&baf);
 		}
 	    for (j=0; jdna[qs+j], tSeq->dna[ts+j]);
 	    lastQe = qs + sz;
 	    lastTe = ts + sz;
 	    }
 	bafFlushLine(&baf);
 
 	fprintf( f, "*Aligned Blocks with gaps <= %d bases are merged for this display\n", maxSkip);
 	}
     }
 fprintf(f, "
"); if (qIsRc) reverseComplement(qSeq->dna, qSeq->size); if (tIsRc) reverseComplement(tSeq->dna, tSeq->size); freeMem(dna); freeMem(qLetters); } int pslGenoShowAlignment(struct psl *psl, boolean isProt, char *qName, bioSeq *qSeq, int qStart, int qEnd, char *tName, bioSeq *tSeq, int tStart, int tEnd, int exnStarts[], int exnEnds[], int exnCnt, FILE *f) /* Show aligned exons between a pre-located gene (a stamper gene)and its homologues (stamp elements) * in the genome. The aligned exon sequences are shown in blue as regular blat alignment. * The unaligned exon sequence are shown in red. Intron sequences are shown in black */ { /* At this step we just do a little shuffling of the strands for * untranslated DNA alignments. */ char origStrand[2]; boolean needsSwap = (psl->strand[0] == '-' && psl->strand[1] == 0); if (needsSwap) { memcpy(origStrand, psl->strand, 2); pslRc(psl); } pslShowAlignmentStranded2(psl, isProt, qName, qSeq, qStart, qEnd, tName, tSeq, tStart, tEnd,exnStarts, exnEnds, exnCnt, f); if (needsSwap) { pslRc(psl); memcpy(psl->strand, origStrand, 2); } return psl->blockCount; }