1084bf14211e7e4ace072d7a213672f3a8f98be1 kate Fri Jul 24 17:30:21 2015 -0700 Scale wrt all genes, not just those in window. We now save the max tissue median score in the gtexInfo table. refs #15645 diff --git src/hg/hgTracks/gtexTracks.c src/hg/hgTracks/gtexTracks.c index c4d9df3..9cfb0a7 100644 --- src/hg/hgTracks/gtexTracks.c +++ src/hg/hgTracks/gtexTracks.c @@ -1,24 +1,25 @@ /* GTEX tracks */ /* Copyright (C) 2015 The Regents of the University of California * See README in this or parent directory for licensing information. */ #include "common.h" #include "hgTracks.h" #include "hvGfx.h" #include "rainbow.h" +#include "gtexInfo.h" #include "gtexGeneBed.h" #include "gtexTissue.h" #include "gtexUi.h" #define WIN_MAX_GRAPH 20000 #define MAX_GRAPH_HEIGHT 100 #define MAX_BAR_WIDTH 5 #define MAX_GRAPH_PADDING 2 #define WIN_MED_GRAPH 500000 #define MED_GRAPH_HEIGHT 60 #define MED_BAR_WIDTH 3 #define MED_GRAPH_PADDING 1 #define MIN_GRAPH_HEIGHT 20 @@ -97,48 +98,58 @@ } static int gtexGraphWidth(struct gtexGeneBed *gtex) /* Width of GTEx graph in pixels */ { int barWidth = gtexBarWidth(); int padding = gtexGraphPadding(); int count = gtex->expCount; return (barWidth * count) + (padding * (count-1)); } static int valToHeight(double val, double maxVal, int maxHeight) /* Convert a value from 0 to maxVal to 0 to maxHeight-1 */ { -double scaled = val/maxVal; +// FIXME: This sort of works. Seems to be dropping some tho. e.g. check MRAP lung +if (val == 0.0) + return 0; +// smallest counts are 1x10e-3, translate to counter negativity +double scaled = (log10(val)+3.001)/(log10(maxVal)+3.001); +if (scaled < 0) + warn("scaled=%f\n", scaled); +//uglyf("%.2f -> %.2f height %d", val, scaled, (int)scaled * (maxHeight-1)); return (scaled * (maxHeight-1)); } +// TODO: whack this static int valToY(double val, double maxVal, int maxHeight) /* Convert a value from 0 to maxVal to 0 to height-1 */ { -double scaled = val/maxVal; +if (val == 0.0) + return 0; +double scaled = (log10(val)+3.001)/(log10(maxVal)+3.001); +if (scaled < 0) + warn("scaled=%f\n", scaled); int y = scaled * (maxHeight); +//uglyf("%.2f -> %.2f height %d", val, scaled, (int)scaled * (maxHeight-1)); return (maxHeight-1) - y; - -//int y = scaled * (height-1); -//return (height - 1) - y; } struct gtexGeneExtras { - double maxExp; // TODO: remove this local normalization factor + double maxMedian; char *graphType; boolean isComparison; }; /* Cache tissue metadata */ struct gtexTissue *getGtexTissues() /* Get tissue metadata from database */ { static struct gtexTissue *gtexTissues = NULL; if (gtexTissues == NULL) { char query[1024]; struct sqlConnection *conn = sqlConnect("hgFixed"); sqlSafef(query, sizeof(query), "select * from gtexTissue order by id"); @@ -241,31 +252,31 @@ return; } struct gtexGeneBed *computedMedians = NULL; // 1 or 2 (if comparison) // with medians computed for sample subsets struct gtexGeneExtras *extras = (struct gtexGeneExtras *)tg->extraUiData; if ((extras->isComparison) && (tg->visibility == tvFull || tg->visibility == tvPack) && gtexGraphHeight() != MIN_GRAPH_HEIGHT) { // compute medians based on configuration (comparisons, and later, filters) computedMedians = loadComputedMedians(geneBed, extras->graphType); } int i; int expCount = geneBed->expCount; -double maxExp = ((struct gtexGeneExtras *)tg->extraUiData)->maxExp; +double maxMedian = ((struct gtexGeneExtras *)tg->extraUiData)->maxMedian; struct rgbColor lineColor = {.r=0}; int lineColorIx = hvGfxFindColorIx(hvg, lineColor.r, lineColor.g, lineColor.b); int heightPer = tg->heightPer; int graphX = gtexGraphX(geneBed); if (graphX < 0) return; // x1 is at left of graph int x1 = xOff + graphX; int startX = x1; // yZero is at bottom of graph int yZero = gtexGraphHeight() + y - 1; // draw faint line under graph to delineate extent when bars are missing (tissue w/ 0 expression) @@ -294,41 +305,38 @@ colors = getRainbow(&saturatedRainbowAtPos, expCount); //colors = getRainbow(&lightRainbowAtPos, expCount); } for (i=0; i<expCount; i++) { struct rgbColor fillColor = colors[i]; if (barWidth == 1 && sameString(colorScheme, GTEX_COLORS_GTEX)) { // brighten colors a bit so they'll be more visible at this scale struct hslColor hsl = mgRgbToHsl(fillColor); hsl.s = min(1000, hsl.s + 300); fillColor = mgHslToRgb(hsl); } int fillColorIx = hvGfxFindColorIx(hvg, fillColor.r, fillColor.g, fillColor.b); double expScore = geneBed->expScores[i]; - int yMedian = valToY(expScore, maxExp, gtexGraphHeight()) + y; - //int foo = valToY(expScore, maxExp, gtexGraphHeight()); - //int yMedian = yZero - height; - int height = yZero - yMedian; - // TODO: adjust yGene instead of yMedian+1 to get gene track distance as desired - //if (i ==0) uglyf("DRAW: expScore=%.2f, maxExp=%.2f, graphHeight=%d, y=%d<br>", expScore, maxExp, gtexGraphHeight(), y); + int height = valToHeight(expScore, maxMedian, gtexGraphHeight()); + // TODO: adjust yGene to get gene track distance as desired + //if (i ==0) uglyf("DRAW: expScore=%.2f, maxMedian=%.2f, graphHeight=%d, y=%d<br>", expScore, maxMedian, gtexGraphHeight(), y); //if (i ==0) uglyf("DRAW: yZero=%d, yMedian=%d, height=%d<br>", yZero, yMedian, height); if (graphPadding == 0 || sameString(colorScheme, GTEX_COLORS_GTEX)) - hvGfxBox(hvg, x1, yMedian+1, barWidth, height, fillColorIx); + hvGfxBox(hvg, x1, yZero-height, barWidth, height, fillColorIx); else - hvGfxOutlinedBox(hvg, x1, yMedian+1, barWidth, height, fillColorIx, lineColorIx); + hvGfxOutlinedBox(hvg, x1, yZero-height, barWidth, height, fillColorIx, lineColorIx); x1 = x1 + barWidth + graphPadding; } // mark gene extent int yGene = yZero + gtexGeneMargin() - 1; // load & draw gene model char query[1024]; char **row; sqlSafef(query, sizeof query, "select * from gtexGeneModel where name='%s'", geneBed->geneId); struct sqlConnection *conn = hAllocConn(database); if (conn == NULL) return; //uglyf("query: %s<br>", query); @@ -357,112 +365,106 @@ x1 = startX; // yZero is at top of graph yZero = yGene + gtexGeneHeight(); for (i=0; i<expCount; i++) { struct rgbColor fillColor = colors[i]; if (barWidth == 1 && sameString(colorScheme, GTEX_COLORS_GTEX)) { // brighten colors a bit so they'll be more visible at this scale struct hslColor hsl = mgRgbToHsl(fillColor); hsl.s = min(1000, hsl.s + 300); fillColor = mgHslToRgb(hsl); } int fillColorIx = hvGfxFindColorIx(hvg, fillColor.r, fillColor.g, fillColor.b); double expScore = geneBed->expScores[i]; - int height = valToHeight(expScore, maxExp, gtexGraphHeight()); + int height = valToHeight(expScore, maxMedian, gtexGraphHeight()); // TODO: adjust yGene instead of yMedian+1 to get gene track distance as desired - //if (i ==0) uglyf("DRAW2: expScore=%.2f, maxExp=%.2f, graphHeight=%d, y=%d<br>", expScore, maxExp, gtexGraphHeight(), y); + //if (i ==0) uglyf("DRAW2: expScore=%.2f, maxMedian=%.2f, graphHeight=%d, y=%d<br>", expScore, maxMedian, gtexGraphHeight(), y); //if (i ==0) uglyf("DRAW2: yZero=%d, height=%d<br>", yZero, height); if (graphPadding == 0 || sameString(colorScheme, GTEX_COLORS_GTEX)) hvGfxBox(hvg, x1, yZero, barWidth, height, fillColorIx); else hvGfxOutlinedBox(hvg, x1, yZero, barWidth, height, fillColorIx, lineColorIx); x1 = x1 + barWidth + graphPadding; } } static void gtexGeneMapItem(struct track *tg, struct hvGfx *hvg, void *item, char *itemName, char *mapItemName, int start, int end, int x, int y, int width, int height) /* Create a map box for each tissue (bar in the graph) or a single map for squish/dense modes */ { //uglyf("map item: itemName=%s, mapItemName=%s, start=%d, end=%d, x=%d, y=%d, width=%d, height=%d, insideX=%d<br>", //itemName, mapItemName, start, end, x, y, width, height, insideX); if (tg->visibility == tvDense || tg->visibility == tvSquish) { genericMapItem(tg, hvg, item, itemName, itemName, start, end, x, y, width, height); } struct gtexTissue *tissues = getGtexTissues(); struct gtexTissue *tissue = NULL; struct gtexGeneBed *gtex = item; int barWidth = gtexBarWidth(); int padding = gtexGraphPadding(); -double maxExp = ((struct gtexGeneExtras *)tg->extraUiData)->maxExp; +double maxMedian = ((struct gtexGeneExtras *)tg->extraUiData)->maxMedian; int graphX = gtexGraphX((struct gtexGeneBed *)item); if (graphX < 0) return; // x1 is at left of graph int x1 = insideX + graphX; int i = 0; int yZero = gtexGraphHeight() + y - 1; for (tissue = tissues; tissue != NULL; tissue = tissue->next, i++) { double expScore = gtex->expScores[i]; - int yMedian = valToY(expScore, maxExp, gtexGraphHeight()) + y; + //TODO: use valToHeight + int yMedian = valToY(expScore, maxMedian, gtexGraphHeight()) + y; int height = yZero - yMedian; // TODO: call genericMapItem //genericMapItem(tg, hvg, item, itemName, tissue->description, start, end, x1, y, barWidth, height); mapBoxHc(hvg, start, end, x1, yMedian+1, barWidth, height, tg->track, mapItemName, tissue->description); - //if (i==0) uglyf("MAP: expScore=%.2f, maxExp=%.2f, graphHeight=%d, y=%d<br>", expScore, maxExp, gtexGraphHeight(), y); + //if (i==0) uglyf("MAP: expScore=%.2f, maxMedian=%.2f, graphHeight=%d, y=%d<br>", expScore, maxMedian, gtexGraphHeight(), y); //if (i==0) uglyf("MAP: x=%d, x1=%d, y=%d, yZero=%d<br>", x, x1, y, yZero); //if (i==0) uglyf("MAP: yZero=%d, yMedian=%d, height=%d<br>", yZero, yMedian, height); x1 = x1 + barWidth + padding; } } static struct gtexGeneBed *loadGtexGeneBed(char **row) { return gtexGeneBedLoad(row); } static void gtexGeneLoadItems(struct track *tg) { bedLoadItem(tg, tg->table, (ItemLoader)loadGtexGeneBed); struct gtexGeneExtras *extras; AllocVar(extras); tg->extraUiData = extras; // TODO: move test to lib char *graphType = cartUsualStringClosestToHome(cart, tg->tdb, FALSE, GTEX_GRAPH, GTEX_GRAPH_DEFAULT); extras->graphType = cloneString(graphType); if (sameString(graphType, GTEX_GRAPH_AGE) || sameString(graphType, GTEX_GRAPH_SEX)) extras->isComparison = TRUE; -double maxExp = 0.0; -int i; -struct gtexGeneBed *geneBed; -// TODO: Remove this fake (window-based) normalization -for (geneBed = tg->items; geneBed != NULL; geneBed = geneBed->next) - for (i=0; i<geneBed->expCount; i++) - maxExp = (geneBed->expScores[i] > maxExp ? geneBed->expScores[i] : maxExp); -extras->maxExp = maxExp; +extras->maxMedian = gtexMaxMedianScore(NULL); } static int gtexGeneItemHeight(struct track *tg, void *item) { if ((item == NULL) || (tg->visibility == tvSquish) || (tg->visibility == tvDense)) return 0; int extra = 0; if (((struct gtexGeneExtras *)tg->extraUiData)->isComparison) extra = gtexGraphHeight() + 2; //uglyf("GTEX itemHeight extra = %d<br>", extra); return gtexGraphHeight() + gtexGeneMargin() + gtexGeneHeight() + extra; } static int gtexTotalHeight(struct track *tg, enum trackVisibility vis) /* Figure out total height of track */