4bb2602dc32b56e79cc661b7c546a05284f9581a kate Wed Jul 22 11:47:30 2015 -0700 Add squished gene model under expression graph (address concern of SAB and others re anchoring to genome. refs #15645 diff --git src/hg/hgTracks/gtexTracks.c src/hg/hgTracks/gtexTracks.c index c883554..8c96182 100644 --- src/hg/hgTracks/gtexTracks.c +++ src/hg/hgTracks/gtexTracks.c @@ -88,32 +88,34 @@ static int gtexGraphHeight() { int winSize = winEnd - winStart; if (winSize < WIN_MAX_GRAPH) return MAX_GRAPH_HEIGHT; else if (winSize < WIN_MED_GRAPH) return MED_GRAPH_HEIGHT; else return MIN_GRAPH_HEIGHT; } static int valToY(double val, double maxVal, int height) /* Convert a value from 0 to maxVal to 0 to height-1 */ { double scaled = val/maxVal; -int y = scaled * (height-1); -return height - 1 - y; +int y = scaled * (height); +return (height-1) - y; +//int y = scaled * (height-1); +//return (height - 1) - y; } struct gtexGeneExtras { double maxExp; }; /* Cache tissue metadata */ struct gtexTissue *getGtexTissues() /* Get tissue metadata from database */ { static struct gtexTissue *gtexTissues = NULL; if (gtexTissues == NULL) @@ -158,198 +160,248 @@ /* Locate graph on X, relative to viewport. Return -1 if it won't fit */ { int start = max(gtex->chromStart, winStart); int end = min(gtex->chromEnd, winEnd); if (start > end) return -1; double scale = scaleForWindow(insideWidth, winStart, winEnd); int x1 = round((start - winStart) * scale); int x2 = round((end - winStart) * scale); int width = gtexGraphWidth(gtex); if (x1 + width > x2) return -1; return x1; } +static int gtexGeneHeight() +{ + return 8; +} + +static int gtexGeneMargin() +{ + return 1; +} + static void gtexGeneDrawAt(struct track *tg, void *item, struct hvGfx *hvg, int xOff, int y, double scale, MgFont *font, Color color, enum trackVisibility vis) { struct gtexGeneBed *geneBed = item; -//warn("item: %s, xOff=%d\n", geneBed->name, xOff); -if (vis != tvFull && vis != tvPack) - { initGeneColors(hvg); +//warn("item: %s, xOff=%d\n", geneBed->name, xOff); // Color using transcriptClass +Color statusColor; if (geneBed->transcriptClass == NULL) - color = statusColors.unknown; - if (sameString(geneBed->transcriptClass, "coding")) - color = statusColors.coding; + statusColor = statusColors.unknown; +else if (sameString(geneBed->transcriptClass, "coding")) + statusColor = statusColors.coding; else if (sameString(geneBed->transcriptClass, "nonCoding")) - color = statusColors.nonCoding; + statusColor = statusColors.nonCoding; else if (sameString(geneBed->transcriptClass, "problem")) - color = statusColors.problem; + statusColor = statusColors.problem; else - color = statusColors.unknown; + statusColor = statusColors.unknown; - bedDrawSimpleAt(tg, item, hvg, xOff, y, scale, font, color, vis); +if (vis != tvFull && vis != tvPack) + { + bedDrawSimpleAt(tg, item, hvg, xOff, y, scale, font, statusColor, vis); return; } int i; int expCount = geneBed->expCount; double maxExp = ((struct gtexGeneExtras *)tg->extraUiData)->maxExp; struct rgbColor lineColor = {.r=0}; int lineColorIx = hvGfxFindColorIx(hvg, lineColor.r, lineColor.g, lineColor.b); int heightPer = tg->heightPer; -////int start = max(geneBed->chromStart, winStart); - -//int geneEnd = min(geneBed->chromEnd, winEnd); -//int width = expCount * (BAR_WIDTH + PADDING); -//int end = min(expCount*(BAR_WIDTH + PADDING), winEnd); -//if (start > end) - //return; -////int x1 = round((start-winStart)*scale) + xOff; +// x1 is at left of graph int x1 = gtexGraphX(geneBed) + xOff; -//warn("gtexGeneDrawAt: x1=%d, labelWidth=%d. ", - //x1, mgFontStringWidth(font, geneBed->name)); -//int x2 = round((e-winStart)*scale) + xOff; -int yBase = valToY(0, maxExp, heightPer) + y; -//int yZero = yBase - 5; +// yZero is at bottom of graph +int yZero = gtexGraphHeight() + y - 1; + +//uglyf("DRAW: xOff=%d, x1=%d, y=%d, yZero=%d<br>", xOff, x1, y, yZero); -//int firstX = x1; int barWidth = gtexBarWidth(); int graphPadding = gtexGraphPadding(); char *colorScheme = cartUsualStringClosestToHome(cart, tg->tdb, FALSE, GTEX_COLORS, GTEX_COLORS_DEFAULT); struct rgbColor *colors; if (sameString(colorScheme, GTEX_COLORS_GTEX)) { // retrieve from table // TODO: cache this colors = getGtexTissueColors(); } else { // currently the only other choice // TODO: cache this colors = getRainbow(&saturatedRainbowAtPos, expCount); //colors = getRainbow(&lightRainbowAtPos, expCount); } for (i=0; i<expCount; i++) { struct rgbColor fillColor = colors[i]; if (barWidth == 1 && sameString(colorScheme, GTEX_COLORS_GTEX)) { // brighten colors a bit so they'll be more visible at this scale struct hslColor hsl = mgRgbToHsl(fillColor); hsl.s = min(1000, hsl.s + 300); fillColor = mgHslToRgb(hsl); } int fillColorIx = hvGfxFindColorIx(hvg, fillColor.r, fillColor.g, fillColor.b); double expScore = geneBed->expScores[i]; - int yMedian = valToY(expScore, maxExp, heightPer) + y; + int yMedian = valToY(expScore, maxExp, gtexGraphHeight()) + y; + //int height = yZero - yMedian + 1; + int height = yZero - yMedian; + // TODO: adjust yGene instead of yMedian+1 to get gene track distance as desired + //if (i ==0) uglyf("DRAW: expScore=%.2f, maxExp=%.2f, graphHeight=%d, y=%d<br>", expScore, maxExp, gtexGraphHeight(), y); + //if (i ==0) uglyf("DRAW: yZero=%d, yMedian=%d, height=%d<br>", yZero, yMedian, height); if (graphPadding == 0 || sameString(colorScheme, GTEX_COLORS_GTEX)) - hvGfxBox(hvg, x1, yMedian, barWidth, yBase - yMedian, fillColorIx); + hvGfxBox(hvg, x1, yMedian+1, barWidth, height, fillColorIx); else - hvGfxOutlinedBox(hvg, x1, yMedian, barWidth, yBase - yMedian, fillColorIx, lineColorIx); - //warn("x1=%d, yMedian=%d, height=%d\n", x1, yMedian, yBase-yMedian); + hvGfxOutlinedBox(hvg, x1, yMedian+1, barWidth, height, fillColorIx, lineColorIx); x1 = x1 + barWidth + graphPadding; } -// underline gene extent -//hvGfxBox(hvg, firstX, yBase, round((geneEnd - start)*scale), 3, lineColorIx); +// mark gene extent +int yGene = yZero + gtexGeneMargin() - 1; + +#ifdef NO_GENE +// underline +int start = max(geneBed->chromStart, winStart); +int end = min(geneBed->chromEnd, winEnd); +if (start > end) + return; +int xStart = round((start - winStart) * scale)+xOff; +int width = round((end-start)*scale); +hvGfxBox(hvg, xStart, yGene, width, gtexGeneHeight(), lineColorIx); +//uglyf("DRAW: xStart=%d, yGene=%d, width=%d, height=%d<br> ", xStart, yGene, width, gtexGeneHeight()); +#endif + +// load & draw gene model -// TODO: replace with cached table hash -//char *tissueTable = "gtexTissue"; -//struct sqlConnection *conn = hAllocConn("hgFixed"); -//hFreeConn(&conn); +char query[1024]; +char **row; +sqlSafef(query, sizeof query, "select * from gtexGeneModel where name='%s'", geneBed->geneId); +struct sqlConnection *conn = hAllocConn(database); +if (conn == NULL) + return; +//uglyf("query: %s<br>", query); +struct sqlResult *sr = sqlGetResult(conn, query); +struct genePred *geneModel = NULL; +if (sr != NULL) + { + if ((row = sqlNextRow(sr)) != NULL) + geneModel = genePredLoad(row); + sqlFreeResult(&sr); + } +if (geneModel == NULL) + return; +struct linkedFeatures *lf = linkedFeaturesFromGenePred(tg, geneModel, FALSE); +tg->heightPer = gtexGeneHeight()+1; +lf->filterColor = statusColor; +linkedFeaturesDrawAt(tg, lf, hvg, xOff, yGene, scale, font, color, tvSquish); +tg->heightPer = heightPer; +hFreeConn(&conn); } static void gtexGeneMapItem(struct track *tg, struct hvGfx *hvg, void *item, char *itemName, char *mapItemName, int start, int end, int x, int y, int width, int height) /* Create a map box for each tissue (bar in the graph) */ { //uglyf("map item: itemName=%s, mapItemName=%s, start=%d, end=%d, x=%d, y=%d, width=%d, height=%d, insideX=%d", //itemName, mapItemName, start, end, x, y, width, height, insideX); struct gtexTissue *tissues = getGtexTissues(); struct gtexTissue *tissue = NULL; struct gtexGeneBed *gtex = item; int barWidth = gtexBarWidth(); int padding = gtexGraphPadding(); -int heightPer = tg->heightPer; double maxExp = ((struct gtexGeneExtras *)tg->extraUiData)->maxExp; -int i = 0; + //int start1 = max(start, winStart); // TODO: support reverse display (hvg->rc) //double scale = scaleForWindow(width, start, end); //int x1 = round((start-winStart)*scale) + x; // skip over label. TODO: check w/ different modes, and look for a better way // map box on label int labelWidth = mgFontStringWidth(tl.font, gtex->name) + 3; -mapBoxHc(hvg, start, end, x, y, labelWidth, height, tg->track, mapItemName, mapItemName); +//mapBoxHc(hvg, start, end, x, y, labelWidth, height, tg->track, mapItemName, mapItemName); int x1 = x + labelWidth; + +int i = 0; +int yZero = gtexGraphHeight() + y - 1; for (tissue = tissues; tissue != NULL; tissue = tissue->next, i++) { - //hvGfxBox(hvg, x1, yMedian, barWidth, yBase - yMedian, fillColorIx); double expScore = gtex->expScores[i]; - int yBase = valToY(0, maxExp, heightPer) + y; - int yMedian = valToY(expScore, maxExp, heightPer) + y; - int height = yBase - yMedian; + int yMedian = valToY(expScore, maxExp, gtexGraphHeight()) + y; + int height = yZero - yMedian; + //hvGfxBox(hvg, x1, yMedian, barWidth, yBase - yMedian, fillColorIx); + //int yBase = valToY(0, maxExp, heightPer) + y; + //int height = yBase - yMedian; // TODO: call genericMapItem //genericMapItem(tg, hvg, item, itemName, tissue->description, start, end, x1, y, barWidth, height); - mapBoxHc(hvg, start, end, x1, yMedian, barWidth, height, tg->track, mapItemName, tissue->description); //uglyf("id=%d, mapItemName= %s, start=%d, end=%d, x1=%d, y=%d (expScore=%.2f, yMedian=%d, yBase=%d), height=%d. ", //i, mapItemName, start, end, x1, y1, expScore, yMedian, yBase, height); + mapBoxHc(hvg, start, end, x1, yMedian+1, barWidth, height, tg->track, mapItemName, tissue->description); + #ifdef DEBUG + if (i ==0) + { + uglyf("MAP: expScore=%.2f, maxExp=%.2f, graphHeight=%d, y=%d<br>", expScore, maxExp, gtexGraphHeight(), y); + uglyf("MAP: yZero=%d, yMedian=%d, height=%d<br>", yZero, yMedian, height); + break; + } + #endif x1 = x1 + barWidth + padding; } } static struct gtexGeneBed *loadGtexGeneBed(char **row) { return gtexGeneBedLoad(row); } static void gtexGeneLoadItems(struct track *track) { bedLoadItem(track, track->table, (ItemLoader)loadGtexGeneBed); double maxExp = 0.0; int i; struct gtexGeneBed *geneBed; for (geneBed = track->items; geneBed != NULL; geneBed = geneBed->next) for (i=0; i<geneBed->expCount; i++) maxExp = (geneBed->expScores[i] > maxExp ? geneBed->expScores[i] : maxExp); struct gtexGeneExtras *extras; AllocVar(extras); extras->maxExp = maxExp; track->extraUiData = extras; } static int gtexGeneItemHeight(struct track *track, void *item) { if ((item == NULL) || (track->visibility == tvSquish) || (track->visibility == tvDense)) return 0; -return gtexGraphHeight(); +return gtexGraphHeight() + + gtexGeneMargin() + gtexGeneHeight(); } static int gtexTotalHeight(struct track *track, enum trackVisibility vis) /* Figure out total height of track */ { int height; if (track->visibility == tvSquish || track->visibility == tvDense) height = 10; else - height = gtexGraphHeight(); + height = gtexGraphHeight() + gtexGeneMargin() + gtexGeneHeight(); return tgFixedTotalHeightOptionalOverflow(track, vis, height, height, FALSE); } void gtexGeneMethods(struct track *track) { track->drawItemAt = gtexGeneDrawAt; track->loadItems = gtexGeneLoadItems; track->mapItem = gtexGeneMapItem; track->itemHeight = gtexGeneItemHeight; track->totalHeight = gtexTotalHeight; }