c4da827b5b232b002a32e2ec07cdb6b2494df4e4 kate Mon May 2 14:23:26 2016 -0700 Add highest and total median expression info to section panel. refs #17244 diff --git src/hg/hgGene/gtex.c src/hg/hgGene/gtex.c index 0b4c216..8326082 100644 --- src/hg/hgGene/gtex.c +++ src/hg/hgGene/gtex.c @@ -1,65 +1,90 @@ /* GTEx - Gene expression from the Genotype Tissue Expression project. */ /* Copyright (C) 2016 The Regents of the University of California * See README in this or parent directory for licensing information. */ #include "common.h" #include "hash.h" #include "linefile.h" #include "dystring.h" #include "cart.h" #include "cheapcgi.h" #include "hdb.h" #include "hgGene.h" #include "gtexInfo.h" +#include "gtexTissue.h" +#include "gtexGeneBed.h" #include "gtexUi.h" +static struct gtexSectionInfo + { + char *ensGene; + float totalLevel; + float highestLevel; + char *highestTissue; + } gtexGeneInfo; + static boolean gtexExists(struct section *section, struct sqlConnection *conn, char *geneId) /* Return TRUE if GTEx data exists for this gene */ { // Lookup geneId in knownToEnsembl to get ENST (w/ version), look that up (w/o version) // in ensGene to get ENSG (w/o version), and save it for printer char query[512]; if (!sqlTableExists(conn, "gtexGene")) return FALSE; sqlSafef(query, sizeof(query), "SELECT ensGene.name2 FROM ensGene, knownToEnsembl WHERE " "knownToEnsembl.name='%s' AND " "ensGene.name=SUBSTRING_INDEX(knownToEnsembl.value,'.',1)", geneId); char *ensGene = sqlQuickString(conn, query); if (!ensGene) return FALSE; +// construct full accession (with version) in gtexGene table sqlSafef(query, sizeof(query), "select geneId from gtexGene where geneId like '%s%%'", ensGene); ensGene = sqlQuickString(conn, query); if (!ensGene) return FALSE; -section->items = cloneString(ensGene); + +// get gtexGene median expression values +sqlSafef(query, sizeof(query), + "SELECT * from gtexGene WHERE geneId='%s'", ensGene); +struct gtexGeneBed *gtexGene = gtexGeneBedLoadByQuery(conn, query); +assert(gtexGene); +gtexGeneInfo.ensGene = cloneString(ensGene); +gtexGeneInfo.totalLevel = gtexGeneTotalMedianExpression(gtexGene); +int tisId; +gtexGeneInfo.highestLevel = gtexGeneHighestMedianExpression(gtexGene, &tisId); +gtexGeneInfo.highestTissue = gtexGetTissueDescription(tisId, NULL); +section->items = >exGeneInfo; return TRUE; } static void gtexPrint(struct section *section, struct sqlConnection *conn, char *geneId) /* Print out GTEx gene expression boxplot */ { struct tempName pngTn; -char *ensGene = section->items; -if (gtexGeneBoxplot(ensGene, NULL, GTEX_DEFAULT_VERSION, FALSE, &pngTn)) +struct gtexSectionInfo *info = section->items; +printf("Highest median expression: %0.2f RPKM in %s\n", + info->highestLevel, info->highestTissue); +printf("
Total median expression: %0.2f RPKM

\n", info->totalLevel); +if (gtexGeneBoxplot(info->ensGene, NULL, GTEX_DEFAULT_VERSION, FALSE, &pngTn)) hPrintf("
\n", pngTn.forHtml); hPrintf("
" "View in GTEx track of Genome Browser", cartSidUrlString(cart)); hPrintf("    "); -gtexPortalLink(ensGene); +gtexPortalLink(info->ensGene); } struct section *gtexSection(struct sqlConnection *conn, struct hash *sectionRa) /* Create GTEx gene expression section. */ { struct section *section = sectionNew(sectionRa, "gtex"); if (section != NULL) { section->exists = gtexExists; section->print = gtexPrint; } return section; }