c4da827b5b232b002a32e2ec07cdb6b2494df4e4
kate
Mon May 2 14:23:26 2016 -0700
Add highest and total median expression info to section panel. refs #17244
diff --git src/hg/hgGene/gtex.c src/hg/hgGene/gtex.c
index 0b4c216..8326082 100644
--- src/hg/hgGene/gtex.c
+++ src/hg/hgGene/gtex.c
@@ -1,65 +1,90 @@
/* GTEx - Gene expression from the Genotype Tissue Expression project. */
/* Copyright (C) 2016 The Regents of the University of California
* See README in this or parent directory for licensing information. */
#include "common.h"
#include "hash.h"
#include "linefile.h"
#include "dystring.h"
#include "cart.h"
#include "cheapcgi.h"
#include "hdb.h"
#include "hgGene.h"
#include "gtexInfo.h"
+#include "gtexTissue.h"
+#include "gtexGeneBed.h"
#include "gtexUi.h"
+static struct gtexSectionInfo
+ {
+ char *ensGene;
+ float totalLevel;
+ float highestLevel;
+ char *highestTissue;
+ } gtexGeneInfo;
+
static boolean gtexExists(struct section *section, struct sqlConnection *conn, char *geneId)
/* Return TRUE if GTEx data exists for this gene */
{
// Lookup geneId in knownToEnsembl to get ENST (w/ version), look that up (w/o version)
// in ensGene to get ENSG (w/o version), and save it for printer
char query[512];
if (!sqlTableExists(conn, "gtexGene"))
return FALSE;
sqlSafef(query, sizeof(query),
"SELECT ensGene.name2 FROM ensGene, knownToEnsembl WHERE "
"knownToEnsembl.name='%s' AND "
"ensGene.name=SUBSTRING_INDEX(knownToEnsembl.value,'.',1)", geneId);
char *ensGene = sqlQuickString(conn, query);
if (!ensGene)
return FALSE;
+// construct full accession (with version) in gtexGene table
sqlSafef(query, sizeof(query),
"select geneId from gtexGene where geneId like '%s%%'", ensGene);
ensGene = sqlQuickString(conn, query);
if (!ensGene)
return FALSE;
-section->items = cloneString(ensGene);
+
+// get gtexGene median expression values
+sqlSafef(query, sizeof(query),
+ "SELECT * from gtexGene WHERE geneId='%s'", ensGene);
+struct gtexGeneBed *gtexGene = gtexGeneBedLoadByQuery(conn, query);
+assert(gtexGene);
+gtexGeneInfo.ensGene = cloneString(ensGene);
+gtexGeneInfo.totalLevel = gtexGeneTotalMedianExpression(gtexGene);
+int tisId;
+gtexGeneInfo.highestLevel = gtexGeneHighestMedianExpression(gtexGene, &tisId);
+gtexGeneInfo.highestTissue = gtexGetTissueDescription(tisId, NULL);
+section->items = >exGeneInfo;
return TRUE;
}
static void gtexPrint(struct section *section, struct sqlConnection *conn, char *geneId)
/* Print out GTEx gene expression boxplot */
{
struct tempName pngTn;
-char *ensGene = section->items;
-if (gtexGeneBoxplot(ensGene, NULL, GTEX_DEFAULT_VERSION, FALSE, &pngTn))
+struct gtexSectionInfo *info = section->items;
+printf("Highest median expression: %0.2f RPKM in %s\n",
+ info->highestLevel, info->highestTissue);
+printf("
Total median expression: %0.2f RPKM
\n", info->totalLevel);
+if (gtexGeneBoxplot(info->ensGene, NULL, GTEX_DEFAULT_VERSION, FALSE, &pngTn))
hPrintf("
\n", pngTn.forHtml);
hPrintf("
"
"View in GTEx track of Genome Browser", cartSidUrlString(cart));
hPrintf(" ");
-gtexPortalLink(ensGene);
+gtexPortalLink(info->ensGene);
}
struct section *gtexSection(struct sqlConnection *conn, struct hash *sectionRa)
/* Create GTEx gene expression section. */
{
struct section *section = sectionNew(sectionRa, "gtex");
if (section != NULL)
{
section->exists = gtexExists;
section->print = gtexPrint;
}
return section;
}