411f45b8bd4a6cde404a78b81e4a41ea0aede025
mspeir
Mon Feb 26 07:51:10 2018 -0800
Adding link to YouTube video about using hgPcr, refs #20952
diff --git src/hg/hgPcr/hgPcr.c src/hg/hgPcr/hgPcr.c
index 91c9bb6..bc41bc1 100644
--- src/hg/hgPcr/hgPcr.c
+++ src/hg/hgPcr/hgPcr.c
@@ -149,30 +149,34 @@
}
dyStringFree(&dy);
sqlFreeResult(&sr);
hDisconnectCentral(&conn);
hFreeConn(&conn2);
slReverse(&serverList);
return serverList;
}
void doHelp()
/* Print up help page */
{
puts(
"In-Silico PCR searches a sequence database with a pair of\n"
"PCR primers, using an indexing strategy for fast performance.\n"
+"See an example\n"
+"video\n"
+"on our YouTube channel.\n"
"\n"
"
Configuration Options
\n"
"Genome and Assembly - The sequence database to search.
\n"
"Target - If available, choose to query transcribed sequences.
\n"
"Forward Primer - Must be at least 15 bases in length.
\n"
"Reverse Primer - On the opposite strand from the forward primer. Minimum length of 15 bases.
\n"
"Max Product Size - Maximum size of amplified region.
\n"
"Min Perfect Match - Number of bases that match exactly on 3' end of primers. Minimum match size is 15.
\n"
"Min Good Match - Number of bases on 3' end of primers where at least 2 out of 3 bases match.
\n"
"Flip Reverse Primer - Invert the sequence order of the reverse primer and complement it.
\n"
"\n"
"Output
\n"
"When successful, the search returns a sequence output file in fasta format \n"
"containing all sequence in the database that lie between and include the \n"
"primer pair. The fasta header describes the region in the database\n"