09f8c57bea92e646e7cc4ac1d20a09d585ffe5e5 jnavarr5 Fri May 3 08:33:57 2019 -0700 Updating http to https for galGal6, uiLinks cronjob. diff --git src/hg/makeDb/trackDb/crispr10K.html src/hg/makeDb/trackDb/crispr10K.html index 68514ef..a9095e5 100644 --- src/hg/makeDb/trackDb/crispr10K.html +++ src/hg/makeDb/trackDb/crispr10K.html @@ -46,31 +46,31 @@

Mouse-over a target site to show predicted specificity and efficiency scores:

  1. The MIT Specificity score summarizes all off-targets into a single number from 0-100. The higher the number, the fewer off-target effects are expected. We recommend guides with an MIT specificity > 50.
  2. The efficiency score tries to predict if a guide leads to rather strong or weak cleavage. According to (Haeussler et al. 2016), the Doench 2016 Efficiency score should be used to select the guide with the highest cleavage efficiency when expressing guides from RNA PolIII Promoters such as U6. Scores are given as percentiles, e.g. "70%" means that 70% of mammalian guides have a score equal or lower than this guide. The raw score number is also shown in parentheses after the percentile.
  3. The Moreno-Mateos 2015 Efficiency +href="https://www.crisprscan.org/">Moreno-Mateos 2015 Efficiency score should be used instead of the Doench 2016 score when transcribing the guide in vitro with a T7 promoter, e.g. for injections in mouse, zebrafish or Xenopus embryos. The Moreno-Mateos score is given in percentiles and the raw value in parentheses, see the note above.

Click onto features to show all scores and predicted off-targets with up to four mismatches. The Out-of-Frame score by Bae et al. 2014 is correlated with the probability that mutations induced by the guide RNA will disrupt the open reading frame. The authors recommend out-of-frame scores > 66 to create knock-outs with a single guide efficiently.

Off-target sites are sorted by the CFD score (Doench et al. 2016). The higher the CFD score, the more likely there is off-target cleavage at that site.