+HERC4, with a minus signal track using Negate values to flip the wiggle display to +emphasize that HERC4 is expressed on the minus strand. This image also displays the signal graphed +in points and a smoothing window of 16 pixels.
Smoothing window: When set to a numerical value, this option determines the size, in pixels, of a smoothing window to be passed over the plot to smooth the edges of the bars or lines. This is equivalent to a trend line calculation on the graph. The default setting is "OFF".
Negate values: When checked, all values in the wiggle are negated, meaning that
positive values become negative and vice-versa. This is useful for wiggles representing
transcription or other activities on the minus strand. Be aware that wiggles with negative values
are drawn in altColor not color as positive values
are. The below image shows ENCODE RNA-seq data around two genes on different strands, SIRT1 and
-HERC4, with a minus signal track using Negate values to flip the wiggle display to emphasize that
-HERC4 expresses on the minus strand. This image also displays the signal graphed in points and a
-smoothing window of 16 pixels.
+HERC4, with a minus signal track using Negate values to flip the wiggle display to
+emphasize that HERC4 is expressed on the minus strand. This image also displays the signal graphed
+in points and a smoothing window of 16 pixels.
Draw y indicator lines (boxed in orange):
