c672b62b59fdc40f5774c1210b74d670514e3130 dschmelt Wed Jul 3 15:45:30 2019 -0700 Adding cutting info and changing name for the crisprAll track #23514 diff --git src/hg/makeDb/trackDb/crisprAll.html src/hg/makeDb/trackDb/crisprAll.html index e95bd6f..9bf9b49 100644 --- src/hg/makeDb/trackDb/crisprAll.html +++ src/hg/makeDb/trackDb/crisprAll.html @@ -1,25 +1,26 @@ <h2>Description</h2> <p> This track shows the DNA sequences targetable by CRISPR RNA guides using the Cas9 enzyme from <em>S. pyogenes</em> (PAM: NGG) over the entire $organism ($db) genome. CRISPR target sites were annotated with predicted specificity (off-target effects) and predicted efficiency (on-target cleavage) by various algorithms through the tool <a href="http://crispor.tefor.net/" -target="_blank">CRISPOR</a>. +target="_blank">CRISPOR</a>. Sp-Cas9 usually cuts double-stranded DNA three or +four basepairs 5' of the PAM site. </p> <h2>Display Conventions and Configuration</h2> <p> The track "CRISPR Targets" shows all potential -NGG target sites across the genome. The target sequence of the guide is shown with a thick (exon) bar. The PAM motif match (NGG) is shown with a thinner bar. Guides are colored to reflect both predicted specificity and efficiency. Specificity reflects the "uniqueness" of a 20mer sequence in the genome; the less unique a sequence is, the more likely it is to cleave other locations of the genome (off-target effects). Efficiency is the frequency of cleavage at the target site (on-target efficiency).</p> <p>Shades of gray stand for sites that are hard to target specifically, as the