e5093b334fe57b5d09b70f5b37aee5c447b4666f mspeir Thu Jun 20 10:00:50 2019 -0700 Rearranging encoded email to remove unneeded space in link. no redmine diff --git src/hg/htdocs/singlecell.html src/hg/htdocs/singlecell.html index 8d6137e..7b781d1 100755 --- src/hg/htdocs/singlecell.html +++ src/hg/htdocs/singlecell.html @@ -1,126 +1,126 @@ /style/hgGtexTrackSettings.css">

Single cell resources at UCSC

Single cell experiments measure tens of thousands of gene loci in thousands of cells at the same time. That resulting data is challenging to visualize. The UCSC Genome Browser is adding published datasets as tracks and an entirely new data exploration tool:

UCSC Cell Browser
Interactively color dimensionality reduction plots by cell annotation or genes
Tabula Muris
Genome Browser track showing single cell RNA-seq gene expression, genome coverage and splice sites

UCSC Cell Browser

The UCSC Cell Browser is a fast, lightweight viewer for single-cell data. You can:

Contact us at - cells@ucsc. - edu to request new datasets or suggest improvements. + cells@ucsc.edu + to request new datasets or suggest improvements. To set up your own cell browser, follow our installation and set up instructions.

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Single cell datasets in the UCSC Genome Browser

Below is a screenshot of a public session that highlights the Tabula Muris gene expression track. The session shows the Sox10 locus. The gene expression track at the top shows that the gene is only well expressed in basal cells (orange), mammary gland (blue) and oligodendrocytes (pink). These cell type names are shown when you hover with the mouse over the bars on the genome browser. The coverage tracks below show that all exons are transcribed in these, but the last exon is also transcribed in stromal cells and some non-coding transcription is seen in goblet cells 3' of the gene. The splicing tracks below show that proper splicing is limited to the four cell types described above. This is also a non-coding region conserved in zebrafish and chicken.