32caec3dbd6130357867cf65a101ee4757f7c259 jnavarr5 Wed Jul 24 16:04:17 2019 -0700 Adding a note about how to find the most up-to-date chrom.sizes file for an assembly with patch sequences, no Redmine. diff --git src/utils/bedToBigBed/bedToBigBed.c src/utils/bedToBigBed/bedToBigBed.c index 6fe2795..d01e772 100644 --- src/utils/bedToBigBed/bedToBigBed.c +++ src/utils/bedToBigBed/bedToBigBed.c @@ -38,30 +38,33 @@ static boolean sizesIs2Bit = FALSE; void usage() /* Explain usage and exit. */ { errAbort( "bedToBigBed v. %s - Convert bed file to bigBed. (BigBed version: %d)\n" "usage:\n" " bedToBigBed in.bed chrom.sizes out.bb\n" "Where in.bed is in one of the ascii bed formats, but not including track lines\n" "and chrom.sizes is a two-column file/URL: \n" "and out.bb is the output indexed big bed file.\n" "If the assembly is hosted by UCSC, chrom.sizes can be a URL like\n" " http://hgdownload.soe.ucsc.edu/goldenPath//bigZips/.chrom.sizes\n" "or you may use the script fetchChromSizes to download the chrom.sizes file.\n" + "If you have bed annotations on patch sequences from NCBI, a more inclusive\n" + "chrom.sizes file can be found using a URL like\n" + " http://hgdownload.soe.ucsc.edu/goldenPath//database/chromInfo.txt.gz\n" "If not hosted by UCSC, a chrom.sizes file can be generated by running\n" "twoBitInfo on the assembly .2bit file.\n" "The in.bed file must be sorted by chromosome,start,\n" " to sort a bed file, use the unix sort command:\n" " sort -k1,1 -k2,2n unsorted.bed > sorted.bed\n" "Sorting must be set to skip Unicode mapping (LC_COLLATE=C).\n" "\n" "options:\n" " -type=bedN[+[P]] : \n" " N is between 3 and 15, \n" " optional (+) if extra \"bedPlus\" fields, \n" " optional P specifies the number of extra fields. Not required, but preferred.\n" " Examples: -type=bed6 or -type=bed6+ or -type=bed6+3 \n" " (see http://genome.ucsc.edu/FAQ/FAQformat.html#format1)\n" " -as=fields.as - If you have non-standard \"bedPlus\" fields, it's great to put a definition\n"