9abe26a72523c34f65aa05a4dde5a9535b408e23 lrnassar Thu Jul 25 16:14:36 2019 -0700 Clarifying psl output to replicate web results #23814 diff --git src/hg/htdocs/FAQ/FAQblat.html src/hg/htdocs/FAQ/FAQblat.html index b94c8e1..94fad92 100755 --- src/hg/htdocs/FAQ/FAQblat.html +++ src/hg/htdocs/FAQ/FAQblat.html @@ -167,30 +167,33 @@ Blat server on a separate port to enable translated Blat (protein searches or translated searches in protein-space).</p> <p> <em>gfClient</em>:</p> <ul> <Li>Set <em>-minScore=0</em> and <em>-minIdentity=0</em>. This will result in some low-scoring, generally spurious hits, but for interactive use it's sufficiently easy to ignore them (because results are sorted by score) and sometimes the low-scoring hits come in handy.</li> </ul> <p> <em>standalone Blat</em>:</p> <ul> <li>Blat search:<br> <code>blat -stepSize=5 -repMatch=2253 -minScore=0 -minIdentity=0 database.2bit query.fa output.psl </code><br> + <li><strong>Note:</strong> To replicate web results, PSL output should be used. BLAT handles + alternative output formats (such as blast8) slightly differently, and this can lead to minor + differences in results; particularly for short alignments. </ul> <p> Notes on repMatch:</p> <ul> <li> The default setting for gfServer dna matches is: repMatch = 1024 * (tileSize/stepSize).</li> <li> The default setting for Blat dna matches is: repMatch = 1024 (if tileSize=11).</li> <Li>To get command-line results that are equivalent to web-based results, repMatch must be specified when using BLAT.</li> </ul> <p> For more information about how to replicate the score and percent identity matches displayed by our web-based Blat, please see this <a href="../FAQ/FAQblat.html#blat4">BLAT FAQ</a>.</p> <p>