3d5b0dec29544546bf7e8be1d923433bc5d28428 hiram Thu Nov 28 11:04:34 2019 -0800 done with regenCho1 chainNet refs #24524 diff --git src/hg/makeDb/doc/mm10.txt src/hg/makeDb/doc/mm10.txt index 2907476..8c185fb 100644 --- src/hg/makeDb/doc/mm10.txt +++ src/hg/makeDb/doc/mm10.txt @@ -18364,15 +18364,82 @@ # real 72m34.088s cat fb.gorGor6.chainMm10Link.txt # 1017872526 bases of 2999027915 (33.940%) in intersection cat fb.gorGor6.chainSynMm10Link.txt # 880983055 bases of 2999027915 (29.376%) in intersection time (doRecipBest.pl -load -workhorse=hgwdev gorGor6 mm10 \ -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & # real 237m38.959s cat fb.gorGor6.chainRBest.Mm10.txt # 883663662 bases of 2999027915 (29.465%) in intersection ############################################################################## +# LASTZ Chinese hamster ovary cell line CHO-K1 regenCho1 +# (DONE - 2019-11-26 - Hiram) + # establish a screen to control this job + mkdir /hive/data/genomes/mm10/bed/lastzRegenCho1.2019-11-26 + cd /hive/data/genomes/mm10/bed/lastzRegenCho1.2019-11-26 + + printf '# Chinese hamster ovary cell line vs mouse +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.00/bin/lastz + +# TARGET: Mouse Mm10 +SEQ1_DIR=/hive/data/genomes/mm10/mm10.2bit +SEQ1_LEN=/hive/data/genomes/mm10/chrom.sizes +SEQ1_CHUNK=20000000 +SEQ1_LAP=10000 +SEQ1_LIMIT=40 + +# QUERY: Chinese hamster ovary cell line CHO-K1 regenCho1 +SEQ2_DIR=/hive/data/genomes/regenCho1/regenCho1.2bit +SEQ2_LEN=/hive/data/genomes/regenCho1/chrom.sizes +SEQ2_CHUNK=10000000 +SEQ2_LIMIT=20 +SEQ2_LAP=0 + +BASE=/hive/data/genomes/mm10/bed/lastzRegenCho1.2019-11-26 +TMPDIR=/dev/shm +' > DEF + + time (doBlastzChainNet.pl -verbose=2 `pwd`/DEF \ + -noDbNameCheck -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > do.log 2>&1 & + # real 340m52.020s + + cat fb.mm10.chainRegenCho1Link.txt + # 1525566783 bases of 2652783500 (57.508%) in intersection + cat fb.mm10.chainSynRegenCho1Link.txt + # 1410851403 bases of 2652783500 (53.184%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev mm10 regenCho1 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 664m46.970s + + cat fb.mm10.chainRBest.RegenCho1.txt + # 1395524606 bases of 2652783500 (52.606%) in intersection + + mkdir /hive/data/genomes/regenCho1/bed/blastz.mm10.swap + cd /hive/data/genomes/regenCho1/bed/blastz.mm10.swap + time (doBlastzChainNet.pl -verbose=2 \ + /hive/data/genomes/mm10/bed/lastzRegenCho1.2019-11-26/DEF \ + -noDbNameCheck -swap -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > swap.log 2>&1 & + # real 101m20.296s + + cat fb.regenCho1.chainMm10Link.txt + # 1522181082 bases of 2266312740 (67.166%) in intersection + cat fb.regenCho1.chainSynMm10Link.txt + # 1397889394 bases of 2266312740 (61.681%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev regenCho1 mm10 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 660m29.571s + + cat fb.regenCho1.chainRBest.Mm10.txt + # 1396267649 bases of 2266312740 (61.610%) in intersection + +##############################################################################