9f28b1b4d9cd670829c4aac01d702d3d78563a79 hiram Tue Nov 26 12:02:53 2019 -0800 chain/net mm10 to gorGor6 done refs #24524 diff --git src/hg/makeDb/doc/mm10.txt src/hg/makeDb/doc/mm10.txt index 6d08727..2907476 100644 --- src/hg/makeDb/doc/mm10.txt +++ src/hg/makeDb/doc/mm10.txt @@ -18297,16 +18297,82 @@ # If being pushed public, update 'Release Notes' in # human/mm10/wgEncodeGencodeSuper.html # edit human/mm10/trackDb.gencode.ra to add new .ra file include make DBS=mm10 ## only if being pushed to RR: # edit all.joiner to add ~/tmp/gencodeVM24.joiner # verify with: pushd /hive/data/genomes/mm10/bed/gencodeVM24 make -f ~/kent/src/hg/makeDb/outside/gencode/gencodeLoad.mk joinerCheck # commit all # if pushing public: add ticket and MARK QA READY -######################################################################### +############################################################################## +# LASTZ Gorilla gorGor6 (DONE - 2019-11-20 - Hiram) + # establish a screen to control this job + screen -S mm10gorGor6 + mkdir /hive/data/genomes/mm10/bed/lastzGorGor6.2019-11-20 + cd /hive/data/genomes/mm10/bed/lastzGorGor6.2019-11-20 + + printf '# mouse vs. gorilla +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.00/bin/lastz + +# TARGET: Mouse Mm10 +SEQ1_DIR=/hive/data/genomes/mm10/mm10.2bit +SEQ1_LEN=/hive/data/genomes/mm10/chrom.sizes +SEQ1_CHUNK=20000000 +SEQ1_LAP=10000 +SEQ1_LIMIT=1 + +# QUERY: gorilla gorGor6 +SEQ2_DIR=/hive/data/genomes/gorGor6/gorGor6.2bit +SEQ2_LEN=/hive/data/genomes/gorGor6/chrom.sizes +SEQ2_CHUNK=10000000 +SEQ2_LIMIT=130 +SEQ2_LAP=0 + +BASE=/hive/data/genomes/mm10/bed/lastzGorGor6.2019-11-20 +TMPDIR=/dev/shm +' > DEF + + time (doBlastzChainNet.pl -verbose=2 `pwd`/DEF \ + -syntenicNet -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > do.log 2>&1 & + # real 518m27.777s + + cat fb.mm10.chainGorGor6Link.txt + # 929953885 bases of 2652783500 (35.056%) in intersection + cat fb.mm10.chainSynGorGor6Link.txt + # 882047357 bases of 2652783500 (33.250%) in intersection + + time (doRecipBest.pl -workhorse=hgwdev -load mm10 gorGor6 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 270m54.749s + + cat fb.mm10.chainRBest.GorGor6.txt + # 885135149 bases of 2652783500 (33.366%) in intersection + + mkdir /hive/data/genomes/gorGor6/bed/blastz.mm10.swap + cd /hive/data/genomes/gorGor6/bed/blastz.mm10.swap + time (doBlastzChainNet.pl -verbose=2 \ + /hive/data/genomes/mm10/bed/lastzGorGor6.2019-11-20/DEF \ + -swap -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=hgwdev -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > swap.log 2>&1 + # real 72m34.088s + + cat fb.gorGor6.chainMm10Link.txt + # 1017872526 bases of 2999027915 (33.940%) in intersection + cat fb.gorGor6.chainSynMm10Link.txt + # 880983055 bases of 2999027915 (29.376%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev gorGor6 mm10 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 237m38.959s + + cat fb.gorGor6.chainRBest.Mm10.txt + # 883663662 bases of 2999027915 (29.465%) in intersection + +##############################################################################