cdd0101336b3399e8d1a204a298b017a2675cc3a hiram Fri Jan 24 10:35:41 2020 -0800 now correctly using this asmHubs directories as toolsDir to obtain commonName listings refs #24748 diff --git src/hg/makeDb/doc/asmHubs/mkAsmStats.pl src/hg/makeDb/doc/asmHubs/mkAsmStats.pl index 223ac45..06f6e58 100755 --- src/hg/makeDb/doc/asmHubs/mkAsmStats.pl +++ src/hg/makeDb/doc/asmHubs/mkAsmStats.pl @@ -1,292 +1,295 @@ #!/usr/bin/env perl use strict; use warnings; use File::stat; my $argc = scalar(@ARGV); if ($argc != 2) { printf STDERR "mkAsmStats Name asmName\n"; printf STDERR "e.g.: mkAsmStats Mammals mammals\n"; exit 255; } my $Name = shift; my $asmHubName = shift; my $home = $ENV{'HOME'}; -my $srcDocDir = "${asmHubName}AsmHub"; -my $asmHubDocDir = "$home/kent/src/hg/makeDb/doc/$srcDocDir"; +my $toolsDir = "$home/kent/src/hg/makeDb/doc/asmHubs"; my $commonNameList = "$asmHubName.asmId.commonName.tsv"; my $commonNameOrder = "$asmHubName.commonName.asmId.orderList.tsv"; my @orderList; # asmId of the assemblies in order from the *.list files # the order to read the different .list files: my $assemblyTotal = 0; # complete list of assemblies in this group my $asmCount = 0; # count of assemblies completed and in the table my $overallNucleotides = 0; my $overallSeqCount = 0; my $overallGapSize = 0; my $overallGapCount = 0; - ############################################################################## # from Perl Cookbook Recipe 2.17, print out large numbers with comma delimiters: ############################################################################## sub commify($) { my $text = reverse $_[0]; $text =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g; return scalar reverse $text } ############################################################################## ### start the HTML output ############################################################################## sub startHtml() { my $timeStamp = `date "+%F"`; chomp $timeStamp; +my $subSetMessage = "subset of $asmHubName only"; +if ($asmHubName eq "vertebrate") { + $subSetMessage = "subset of other ${asmHubName}s only"; +} + print <<"END" <!DOCTYPE HTML 4.01 Transitional> <!--#set var="TITLE" value="$Name genomes assembly hubs" --> <!--#set var="ROOT" value="../.." --> <!--#include virtual="\$ROOT/inc/gbPageStartHardcoded.html" --> <h1>$Name Genomes assembly hubs</h1> <p> -Assemblies from NCBI/Genbank/Refseq sources, subset of $asmHubName only. +Assemblies from NCBI/Genbank/Refseq sources, $subSetMessage. </p> <h3>See also: <a href='index.html'>hub access</a></h3><br> <h3>Data resource links</h3> NOTE: <em>Click on the column headers to sort the table by that column</em><br> The <em>link to genome browser</em> will attach only that single assembly to the genome browser. END } ############################################################################## ### start the table output ############################################################################## sub startTable() { print <<"END" <table class="sortable" border="1"> <thead><tr><th>count</th> <th>common name<br>link to genome browser</th> <th>scientific name<br>and data download</th> <th>NCBI assembly</th> <th>sequence<br>count</th><th>genome size<br>nucleotides</th> <th>gap<br>count</th><th>unknown bases<br>(gap size sum)</th><th>masking<br>percent</th> </tr></thead><tbody> END } ############################################################################## ### end the table output ############################################################################## sub endTable() { my $commaNuc = commify($overallNucleotides); my $commaSeqCount = commify($overallSeqCount); my $commaGapSize = commify($overallGapSize); my $commaGapCount = commify($overallGapCount); my $percentDone = 100.0 * $asmCount / $assemblyTotal; my $doneMsg = ""; if ($asmCount < $assemblyTotal) { $doneMsg = sprintf(" (%d build completed, %.2f %% finished)", $asmCount, $percentDone); } print <<"END" </tbody> <tfoot><tr><th>TOTALS:</th><td align=center colspan=3>total assembly count ${assemblyTotal}${doneMsg}</td> <td align=right>$commaSeqCount</td> <td align=right>$commaNuc</td> <td align=right>$commaGapCount</td> <td align=right>$commaGapSize</td> <td colspan=1> </td> </tr></tfoot> </table> END } ############################################################################## ### end the HTML output ############################################################################## sub endHtml() { printf "<p>\nOther assembly hubs available:<br>\n<table border='1'><thead>\n<tr>"; printf "<th><a href='../primates/asmStatsPrimates.html'>Primates</a></th>\n" if ($asmHubName ne "primates"); printf "<th><a href='../mammals/asmStatsMammals.html'>Mammals</a></th>\n" if ($asmHubName ne "mammals"); printf "<th><a href='../birds/asmStatsBirds.html'>Birds</a></th>\n" if ($asmHubName ne "birds"); printf "<th><a href='../fish/asmStatsFish.html'>Fish</a></th>\n" if ($asmHubName ne "fish"); printf "<th><a href='../vertebrate/asmStatsVertebrate.html'>other vertebrates</a></th>\n" if ($asmHubName ne "vertebrate"); printf "</tr></thead>\n</table>\n</p>\n"; print <<"END" </div><!-- closing gbsPage from gbPageStartHardcoded.html --> </div><!-- closing container-fluid from gbPageStartHardcoded.html --> <!--#include virtual="\$ROOT/inc/gbFooterHardcoded.html"--> <script type="text/javascript" src="/js/sorttable.js"></script> </body></html> END } sub asmCounts($) { my ($chromSizes) = @_; my ($sequenceCount, $totalSize) = split('\s+', `ave -col=2 $chromSizes | egrep "^count|^total" | awk '{printf "%d\\n", \$NF}' | xargs echo`); return ($sequenceCount, $totalSize); } # my ($gapSize) = maskStats($faSizeTxt); sub maskStats($) { my ($faSizeFile) = @_; my $gapSize = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$3}'`; chomp $gapSize; $gapSize =~ s/\(//; my $totalBases = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$1}'`; chomp $totalBases; my $maskedBases = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$9}'`; chomp $maskedBases; my $maskPerCent = 100.0 * $maskedBases / $totalBases; return ($gapSize, $maskPerCent); } # grep "sequences in 1 file" GCA_900324465.2_fAnaTes1.2.faSize.txt # 555641398 bases (3606496 N's 552034902 real 433510637 upper 118524265 lower) in 50 sequences in 1 files sub gapStats($$) { my ($buildDir, $asmId) = @_; my $gapBed = "$buildDir/trackData/allGaps/$asmId.allGaps.bed.gz"; my $gapCount = 0; if ( -s "$gapBed" ) { $gapCount = `zcat $gapBed | awk '{print \$3-\$2}' | ave stdin | grep '^count' | awk '{print \$2}'`; } chomp $gapCount; return ($gapCount); } ############################################################################## ### tableContents() ############################################################################## sub tableContents() { foreach my $asmId (reverse(@orderList)) { my $accessionDir = substr($asmId, 0 ,3); $accessionDir .= "/" . substr($asmId, 4 ,3); $accessionDir .= "/" . substr($asmId, 7 ,3); $accessionDir .= "/" . substr($asmId, 10 ,3); $accessionDir .= "/" . $asmId; my $buildDir = "/hive/data/genomes/asmHubs/refseqBuild/$accessionDir"; my $asmReport="$buildDir/download/${asmId}_assembly_report.txt"; next if (! -s "$asmReport"); my ($gcPrefix, $asmAcc, $asmName) = split('_', $asmId, 3); my $chromSizes = "${buildDir}/${asmId}.chrom.sizes"; my $twoBit = "${buildDir}/trackData/addMask/${asmId}.masked.2bit"; next if (! -s "$twoBit"); my $faSizeTxt = "${buildDir}/${asmId}.faSize.txt"; if ( ! -s "$faSizeTxt" ) { printf STDERR "twoBitToFa $twoBit stdout | faSize stdin > $faSizeTxt\n"; print `twoBitToFa $twoBit stdout | faSize stdin > $faSizeTxt`; } my ($gapSize, $maskPerCent) = maskStats($faSizeTxt); $overallGapSize += $gapSize; my ($seqCount, $totalSize) = asmCounts($chromSizes); $overallSeqCount += $seqCount; # my $totalSize=`ave -col=2 $chromSizes | grep "^total" | awk '{printf "%d", \$NF}'`; $overallNucleotides += $totalSize; my $gapCount = gapStats($buildDir, $asmId); $overallGapCount += $gapCount; my $sciName = "notFound"; my $commonName = "notFound"; my $bioSample = "notFound"; my $bioProject = "notFound"; my $taxId = "notFound"; my $asmDate = "notFound"; my $itemsFound = 0; open (FH, "<$asmReport") or die "can not read $asmReport"; while (my $line = <FH>) { last if ($itemsFound > 5); chomp $line; $line =~ s/ //g;; $line =~ s/\s+$//g;; if ($line =~ m/Date:/) { if ($asmDate =~ m/notFound/) { ++$itemsFound; $asmDate = $line; $asmDate =~ s/.*:\s+//; } } elsif ($line =~ m/BioSample:/) { if ($bioSample =~ m/notFound/) { ++$itemsFound; $bioSample = $line; $bioSample =~ s/.*:\s+//; } } elsif ($line =~ m/BioProject:/) { if ($bioProject =~ m/notFound/) { ++$itemsFound; $bioProject = $line; $bioProject =~ s/.*:\s+//; } } elsif ($line =~ m/Organism name:/) { if ($sciName =~ m/notFound/) { ++$itemsFound; $commonName = $line; $sciName = $line; $commonName =~ s/.*\(//; $commonName =~ s/\)//; $sciName =~ s/.*:\s+//; $sciName =~ s/\s+\(.*//; } } elsif ($line =~ m/Taxid:/) { if ($taxId =~ m/notFound/) { ++$itemsFound; $taxId = $line; $taxId =~ s/.*:\s+//; } } } close (FH); my $hubUrl = "https://hgdownload.soe.ucsc.edu/hubs/$accessionDir"; printf "<tr><td align=right>%d</td>\n", ++$asmCount; printf "<td align=center><a href='https://genome.ucsc.edu/cgi-bin/hgGateway?hubUrl=%s/%s.hub.txt&genome=%s&position=lastDbPos' target=_blank>%s</a></td>\n", $hubUrl, $asmId, $asmId, $commonName; printf " <td align=center><a href='https://hgdownload.soe.ucsc.edu/hubs/%s/genomes/%s/' target=_blank>%s</a></td>\n", $asmHubName, $asmId, $sciName; printf " <td align=left><a href='https://www.ncbi.nlm.nih.gov/assembly/%s_%s/' target=_blank>%s</a></td>\n", $gcPrefix, $asmAcc, $asmId; printf " <td align=right>%s</td>\n", commify($seqCount); printf " <td align=right>%s</td>\n", commify($totalSize); printf " <td align=right>%s</td>\n", commify($gapCount); printf " <td align=right>%s</td>\n", commify($gapSize); printf " <td align=right>%.2f</td>\n", $maskPerCent; printf "</tr>\n"; } } ############################################################################## ### main() ############################################################################## -open (FH, "<$asmHubDocDir/${commonNameOrder}") or die "can not read ${commonNameOrder}"; +open (FH, "<$toolsDir/${commonNameOrder}") or die "can not read ${commonNameOrder}"; while (my $line = <FH>) { chomp $line; my ($commonName, $asmId) = split('\t', $line); push @orderList, $asmId; ++$assemblyTotal; } close (FH); startHtml(); startTable(); tableContents(); endTable(); endHtml();