19d33fe8c7b5b90b7faa514185c79eb6af0d8d8b hiram Tue Jan 21 13:22:47 2020 -0800 now using new style GCF path names and separate hubs for each assembly refs #24748 diff --git src/hg/makeDb/doc/primateAsmHub/mkHubIndex.pl src/hg/makeDb/doc/primateAsmHub/mkHubIndex.pl index b330087..f1528d3 100755 --- src/hg/makeDb/doc/primateAsmHub/mkHubIndex.pl +++ src/hg/makeDb/doc/primateAsmHub/mkHubIndex.pl @@ -1,237 +1,244 @@ #!/usr/bin/env perl use strict; use warnings; my $home = $ENV{'HOME'}; my $srcDocDir = "primateAsmHub"; my $asmHubDocDir = "$home/kent/src/hg/makeDb/doc/$srcDocDir"; -my $Name = "Primates"; +my $Name = "Primate"; my $asmHubName = "primates"; my $defaultAssembly = "GCF_000001405.39_GRCh38.p13"; my $srcDir = "$home/kent/src/hg/makeDb/doc/$srcDocDir"; -my $commonNameList = "primates.asmId.commonName.tsv"; my $commonNameOrder = "primates.commonName.asmId.orderList.tsv"; my @orderList; # asmId of the assemblies in order from the *.list files # the order to read the different .list files: -my @classList = qw( human ); -my %class; # key is asmId, value is from class list my $assemblyCount = 0; ############################################################################## # from Perl Cookbook Recipe 2.17, print out large numbers with comma delimiters: ############################################################################## sub commify($) { my $text = reverse $_[0]; $text =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g; return scalar reverse $text } ############################################################################## ### start the HTML output ############################################################################## sub startHtml() { my $timeStamp = `date "+%F"`; chomp $timeStamp; # <html xmlns="http://www.w3.org/1999/xhtml"> print <<"END" <!DOCTYPE HTML 4.01 Transitional> -<!--#set var="TITLE" value="Primate genomes assembly hubs" --> +<!--#set var="TITLE" value="$Name genomes assembly hubs" --> <!--#set var="ROOT" value="../.." --> <!--#include virtual="\$ROOT/inc/gbPageStartHardcoded.html" --> -<h1>Primate Genomes assembly hubs</h1> +<h1>$Name Genomes assembly hubs</h1> <p> -Assemblies from NCBI/Genbank/Refseq sources +Assemblies from NCBI/Genbank/Refseq sources, subset of $asmHubName only. </p> <h3>How to view the hub</h3> <p> -You can load this hub from our +Individual assemblies are attached to the genome browser via the +<em>link to genome browser</em> in the table below. To attach all +of these assemblies in one set for this hub, select the ${Name}s assembly +hub from our <a href="https://genome.ucsc.edu/cgi-bin/hgHubConnect#publicHubs" target="_blank">Public Hubs</a> page or by clicking these assembly links to any of our official websites: <ul> <li> <a href="https://genome.ucsc.edu/cgi-bin/hgGateway?hubUrl=https://hgdownload.soe.ucsc.edu/hubs/$asmHubName/hub.txt&genome=$defaultAssembly" target="_blank">genome.ucsc.edu</a></li> <li> <a href="https://genome-euro.ucsc.edu/cgi-bin/hgGateway?hubUrl=https://hgdownload.soe.ucsc.edu/hubs/$asmHubName/hub.txt&genome=$defaultAssembly" target="_blank">genome-euro.ucsc.edu</a></li> <li> <a href="https://genome-asia.ucsc.edu/cgi-bin/hgGateway?hubUrl=https://hgdownload.soe.ucsc.edu/hubs/$asmHubName/hub.txt&genome=$defaultAssembly" target="_blank">genome-asia.ucsc.edu</a></li> </ul> </p> <p> -To manually attach this hub to other genome browsers: +To manually attach all the assemblies in this hub to other genome browsers: <ol> <li> From the blue navigation bar, go to <em><strong>My Data</strong> -> <strong>Track Hubs</strong></em></li> <li> Then select the <strong>My Hubs</strong> tab and enter this URL into the textbox: <br><code>https://hgdownload.soe.ucsc.edu/hubs/$asmHubName/hub.txt</code></li> <li> Once you have added the URL to the entry form, press the <em><strong>Add Hub</strong></em> button to add the hub.</li> </ol> </p> <p> After adding the hub, you will be redirected to the gateway page. The -genome assemblies can be selected from the <em>Reference Genome Improvement Hub Assembly</em> dropdown menu. -</p> -<p> -<h3>See also: <a href='asmStats$Name.html' target=_blank>assembly statistics</a></h3> +genome assemblies can be selected from the +<em>${Name}s Hub Assembly</em> dropdown menu. +Instead of adding all the assemblies in one collected group, use the individual +<em>link to genome browser</em> in the table below. </p> +<h3>See also: <a href='asmStats$Name.html' +target=_blank>assembly statistics</a></h3><br> <h3>Data resource links</h3> -NOTE: <em>Click on the column headers to sort the table by that column</em> +NOTE: <em>Click on the column headers to sort the table by that column</em><br> +The <em>link to genome browser</em> will attach only that single assembly to +the genome browser. END } # sub startHtml() ############################################################################## ### start the table output ############################################################################## sub startTable() { print <<"END" <table class="sortable" border="1"> <thead><tr><th>count</th> <th>common name<br>link to genome browser</th> <th>scientific name<br>and data download</th> <th>NCBI assembly</th> <th>bioSample</th><th>bioProject</th> <th>assembly date,<br>source link</th> </tr></thead><tbody> END } # sub startTable() ############################################################################## ### end the table output ############################################################################## sub endTable() { print <<"END" </tbody> </table> END } # sub endTable() ############################################################################## ### end the HTML output ############################################################################## sub endHtml() { print <<"END" </div><!-- closing gbsPage from gbPageStartHardcoded.html --> </div><!-- closing container-fluid from gbPageStartHardcoded.html --> <!--#include virtual="\$ROOT/inc/gbFooterHardcoded.html"--> <script type="text/javascript" src="/js/sorttable.js"></script> </body></html> END } # sub endHtml() ############################################################################## ### tableContents() ############################################################################## sub tableContents() { my $rowCount = 0; foreach my $asmId (@orderList) { - my $buildDir = "/hive/data/genomes/asmHubs/refseqBuild/" . substr($asmId, 0 ,3); - $buildDir .= "/" . substr($asmId, 4 ,3); - $buildDir .= "/" . substr($asmId, 7 ,3); - $buildDir .= "/" . substr($asmId, 10 ,3); - $buildDir .= "/" . $asmId; + my $accessionDir = substr($asmId, 0 ,3); + $accessionDir .= "/" . substr($asmId, 4 ,3); + $accessionDir .= "/" . substr($asmId, 7 ,3); + $accessionDir .= "/" . substr($asmId, 10 ,3); + $accessionDir .= "/" . $asmId; + my $ncbiFtpLink = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/$accessionDir/"; + my $buildDir = "/hive/data/genomes/asmHubs/refseqBuild/$accessionDir"; my $asmReport="$buildDir/download/${asmId}_assembly_report.txt"; my ($gcPrefix, $asmAcc, $asmName) = split('_', $asmId, 3); my $chromSizes="${buildDir}/${asmId}.chrom.sizes"; my $sciName = "notFound"; my $commonName = "notFound"; my $bioSample = "notFound"; my $bioProject = "notFound"; my $taxId = "notFound"; my $asmDate = "notFound"; my $itemsFound = 0; open (FH, "<$asmReport") or die "can not read $asmReport"; while (my $line = <FH>) { last if ($itemsFound > 5); chomp $line; $line =~ s/ //g;; $line =~ s/\s+$//g;; if ($line =~ m/Date:/) { if ($asmDate =~ m/notFound/) { ++$itemsFound; $line =~ s/.*:\s+//; my @a = split('-', $line); $asmDate = sprintf("%04d-%02d-%02d", $a[0], $a[1], $a[2]); } } elsif ($line =~ m/BioSample:/) { if ($bioSample =~ m/notFound/) { ++$itemsFound; $bioSample = $line; $bioSample =~ s/.*:\s+//; } } elsif ($line =~ m/BioProject:/) { if ($bioProject =~ m/notFound/) { ++$itemsFound; $bioProject = $line; $bioProject =~ s/.*:\s+//; } } elsif ($line =~ m/Organism name:/) { if ($sciName =~ m/notFound/) { ++$itemsFound; $commonName = $line; $sciName = $line; $commonName =~ s/.*\(//; $commonName =~ s/\)//; $sciName =~ s/.*:\s+//; $sciName =~ s/\s+\(.*//; } } elsif ($line =~ m/Taxid:/) { if ($taxId =~ m/notFound/) { ++$itemsFound; $taxId = $line; $taxId =~ s/.*:\s+//; } } } close (FH); + my $hubUrl = "https://hgdownload.soe.ucsc.edu/hubs/$accessionDir"; printf "<tr><td align=right>%d</td>\n", ++$rowCount; - printf "<td align=center><a href='https://genome.ucsc.edu/cgi-bin/hgGateway?hubUrl=https://hgdownload.soe.ucsc.edu/hubs/%s/hub.txt&genome=%s&position=lastDbPos' target=_blank>%s</a></td>\n", $asmHubName, $asmId, $commonName; - printf " <td align=center><a href='https://hgdownload.soe.ucsc.edu/hubs/%s/genomes/%s/' target=_blank>%s</a></td>\n", $asmHubName, $asmId, $sciName; + printf "<td align=center><a href='https://genome.ucsc.edu/cgi-bin/hgGateway?hubUrl=%s/%s.hub.txt&genome=%s&position=lastDbPos' target=_blank>%s</a></td>\n", $hubUrl, $asmId, $asmId, $commonName; + printf " <td align=center><a href='%s/' target=_blank>%s</a></td>\n", $hubUrl, $sciName; printf " <td align=left><a href='https://www.ncbi.nlm.nih.gov/assembly/%s_%s/' target=_blank>%s</a></td>\n", $gcPrefix, $asmAcc, $asmId; if ( $bioSample ne "notFound" ) { printf " <td align=left><a href='https://www.ncbi.nlm.nih.gov/biosample/?term=%s' target=_blank>%s</a></td>\n", $bioSample, $bioSample; } else { printf " <td align=left>n/a</td>\n"; } printf " <td align=left><a href='https://www.ncbi.nlm.nih.gov/bioproject/?term=%s' target=_blank>%s</a></td>\n", $bioProject, $bioProject; - printf " <td align=center>%s</td>\n", $asmDate; + printf " <td align=center><a href='%s' target=_blank>%s</a></td>\n", $ncbiFtpLink, $asmDate; printf "</tr>\n"; } } # sub tableContents() ############################################################################## ### main() ############################################################################## open (FH, "<$srcDir/${commonNameOrder}") or die "can not read ${commonNameOrder}"; while (my $line = <FH>) { chomp $line; my ($commonName, $asmId) = split('\t', $line); push @orderList, $asmId; ++$assemblyCount; } close (FH); startHtml(); startTable(); tableContents(); endTable(); endHtml();