32bd9b7bea2ca6154383f124a803fdd6ad04c77b hiram Sat Feb 29 16:41:43 2020 -0800 add allGaps column and use featureBits result when present and zero when track is empty refs #23891 diff --git src/hg/makeDb/doc/asmHubs/trackData.pl src/hg/makeDb/doc/asmHubs/trackData.pl index 074214a..326e842 100755 --- src/hg/makeDb/doc/asmHubs/trackData.pl +++ src/hg/makeDb/doc/asmHubs/trackData.pl @@ -27,51 +27,71 @@ my $asmCount = 0; # count of assemblies completed and in the table my $overallNucleotides = 0; my $overallSeqCount = 0; my $overallGapSize = 0; my $overallGapCount = 0; ############################################################################## # from Perl Cookbook Recipe 2.17, print out large numbers with comma delimiters: ############################################################################## sub commify($) { my $text = reverse $_[0]; $text =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g; return scalar reverse $text } -sub oneTrackData($$) { - my ($file, $genomeSize) = @_; +# ($itemCount, $percentCover) = oneTrackData($trackFile, $sizeNoGaps, $trackFb); +# might have a track feature bits file (trackFb), maybe not +sub oneTrackData($$$$$$) { + my ($asmId, $trackName, $file, $genomeSize, $trackFb, $runDir) = @_; # printf STDERR "# %s\n", $file; my $itemCount = 0; my $percentCover = 0; if (! -s "${file}") { + if ($trackName eq "gapOverlap") { + if (-s "${runDir}/$asmId.gapOverlap.bed.gz" ) { + my $lineCount=`zcat "${runDir}/$asmId.gapOverlap.bed.gz" | head | wc -l`; + chomp $lineCount; + if (0 == $lineCount) { + return("0", "0 %"); + } else { return("n/a", "n/a"); } + } + } else { + return("n/a", "n/a"); + } + } if ($file =~ m/.bw$/) { my $bigWigInfo = `bigWigInfo "$file" | egrep "basesCovered:|mean:" | awk '{print \$NF}' | xargs echo | sed -e 's/,//g;'`; chomp $bigWigInfo; my ($bases, $mean) = split('\s+', $bigWigInfo); $percentCover = sprintf("%.2f %%", 100.0 * $bases / $genomeSize); $itemCount = sprintf ("%.2f", $mean); # printf STDERR "# bigWigInfo %s %s %s\n", $itemCount, $percentCover, $file; } else { my $bigBedInfo = `bigBedInfo "$file" | egrep "itemCount:|basesCovered:" | awk '{print \$NF}' | xargs echo | sed -e 's/,//g;'`; chomp $bigBedInfo; my ($items, $bases) = split('\s', $bigBedInfo); $itemCount = commify($items); $percentCover = sprintf("%.2f %%", 100.0 * $bases / $genomeSize); +# 56992654 bases of 2616369673 (2.178%) in intersection + if ( -s "${trackFb}" ) { +printf STDERR "# $trackFb\n"; + my ($itemBases, undef, undef, $noGapSize, undef) = split('\s+', `cat $trackFb`, 5); + $percentCover = sprintf("%.2f %%", 100.0 * $itemBases / $noGapSize); + } # printf STDERR "# bigBedInfo %s %s %s\n", $itemCount, $percentCover, $file; } return ($itemCount, $percentCover); } ############################################################################## ### start the HTML output ############################################################################## sub startHtml() { my $timeStamp = `date "+%F"`; chomp $timeStamp; my $subSetMessage = "subset of $asmHubName only"; if ($asmHubName eq "vertebrate") { @@ -98,40 +118,41 @@ the genome browser.<br> The numbers are: item count (percent coverage)<br> Except for the gc5Base column which is: overall GC % average (percent coverage) END } ############################################################################## ### start the table output ############################################################################## sub startTable() { print <<"END" <table class="sortable" border="1"> <thead><tr><th>count</th> <th>common name<br>link to genome browser</th> <th class="sorttable_numeric">gc5 base</th> - <th class="sorttable_numeric">gap</th> - <th class="sorttable_numeric">assembly</th> + <th class="sorttable_numeric">AGP<br>gap</th> + <th class="sorttable_numeric">all<br>gaps</th> + <th class="sorttable_numeric">assembly<br>sequences</th> <th class="sorttable_numeric">rmsk</th> <th class="sorttable_numeric">TRF<br>simpleRepeat</th> - <th class="sorttable_numeric">windowMasker</th> - <th class="sorttable_numeric">gapOverlap</th> - <th class="sorttable_numeric">tandemDups</th> + <th class="sorttable_numeric">window<br>Masker</th> + <th class="sorttable_numeric">gap<br>Overlap</th> + <th class="sorttable_numeric">tandem<br>Dups</th> <th class="sorttable_numeric">cpg<br>unmasked</th> <th class="sorttable_numeric">cpg<br>island</th> - <th class="sorttable_numeric">ncbiGene</th> + <th class="sorttable_numeric">genes<br>ncbi</th> <th class="sorttable_numeric">ncbiRefSeq</th> <th class="sorttable_numeric">xenoRefGene</th> <th class="sorttable_numeric">augustus</th> </tr></thead><tbody> END } ############################################################################## ### end the table output ############################################################################## sub endTable() { my $commaNuc = commify($overallNucleotides); my $commaSeqCount = commify($overallSeqCount); my $commaGapSize = commify($overallGapSize); @@ -181,96 +202,97 @@ print <<"END" </div><!-- closing gbsPage from gbPageStartHardcoded.html --> </div><!-- closing container-fluid from gbPageStartHardcoded.html --> <!--#include virtual="\$ROOT/inc/gbFooterHardcoded.html"--> <script type="text/javascript" src="/js/sorttable.js"></script> </body></html> END } sub asmCounts($) { my ($chromSizes) = @_; my ($sequenceCount, $totalSize) = split('\s+', `ave -col=2 $chromSizes | egrep "^count|^total" | awk '{printf "%d\\n", \$NF}' | xargs echo`); return ($sequenceCount, $totalSize); } -# my ($gapSize) = maskStats($faSizeTxt); +# my ($gapSize, $maskPerCent, $sizeNoGaps) = maskStats($faSizeTxt); sub maskStats($) { my ($faSizeFile) = @_; + my $sizeNoGaps = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$4}'`; my $gapSize = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$3}'`; chomp $gapSize; $gapSize =~ s/\(//; my $totalBases = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$1}'`; chomp $totalBases; my $maskedBases = `grep 'sequences in 1 file' $faSizeFile | awk '{print \$9}'`; chomp $maskedBases; my $maskPerCent = 100.0 * $maskedBases / $totalBases; - return ($gapSize, $maskPerCent); + return ($gapSize, $maskPerCent, $sizeNoGaps); } # grep "sequences in 1 file" GCA_900324465.2_fAnaTes1.2.faSize.txt # 555641398 bases (3606496 N's 552034902 real 433510637 upper 118524265 lower) in 50 sequences in 1 files sub gapStats($$) { my ($buildDir, $asmId) = @_; my $gapBed = "$buildDir/trackData/allGaps/$asmId.allGaps.bed.gz"; my $gapCount = 0; if ( -s "$gapBed" ) { $gapCount = `zcat $gapBed | awk '{print \$3-\$2}' | ave stdin | grep '^count' | awk '{print \$2}'`; } chomp $gapCount; return ($gapCount); } ############################################################################## ### tableContents() ############################################################################## sub tableContents() { - my @trackList = qw(gc5Base gap assembly rmsk simpleRepeat windowMasker gapOverlap tandemDups cpgIslandExtUnmasked cpgIslandExt ncbiGene ncbiRefSeq xenoRefGene augustus); + my @trackList = qw(gc5Base gap allGaps assembly rmsk simpleRepeat windowMasker gapOverlap tandemDups cpgIslandExtUnmasked cpgIslandExt ncbiGene ncbiRefSeq xenoRefGene augustus); foreach my $asmId (reverse(@orderList)) { my ($gcPrefix, $asmAcc, $asmName) = split('_', $asmId, 3); my $accessionId = sprintf("%s_%s", $gcPrefix, $asmAcc); my $accessionDir = substr($asmId, 0 ,3); $accessionDir .= "/" . substr($asmId, 4 ,3); $accessionDir .= "/" . substr($asmId, 7 ,3); $accessionDir .= "/" . substr($asmId, 10 ,3); my $buildDir = "/hive/data/genomes/asmHubs/refseqBuild/$accessionDir/$asmId"; # next if ($asmId ne "GCF_000001405.39_GRCh38.p13"); my $asmReport="$buildDir/download/${asmId}_assembly_report.txt"; if (! -s "$asmReport") { printf STDERR "# no assembly report:\n# %s\n", $asmReport; next; } my $chromSizes = "${buildDir}/${asmId}.chrom.sizes"; my $twoBit = "${buildDir}/trackData/addMask/${asmId}.masked.2bit"; if (! -s "$twoBit") { printf STDERR "# no 2bit file:\n# %s\n", $twoBit; printf "<tr><td align=right>%d</td>\n", ++$asmCount; printf "<td align=center>%s</td>\n", $accessionId; - printf "<th colspan=14 align=center>missing masked 2bit file</th>\n"; + printf "<th colspan=15 align=center>missing masked 2bit file</th>\n"; printf "</tr>\n"; next; } my $faSizeTxt = "${buildDir}/${asmId}.faSize.txt"; if ( ! -s "$faSizeTxt" ) { printf STDERR "twoBitToFa $twoBit stdout | faSize stdin > $faSizeTxt\n"; print `twoBitToFa $twoBit stdout | faSize stdin > $faSizeTxt`; } - my ($gapSize, $maskPerCent) = maskStats($faSizeTxt); + my ($gapSize, $maskPerCent, $sizeNoGaps) = maskStats($faSizeTxt); $overallGapSize += $gapSize; my ($seqCount, $totalSize) = asmCounts($chromSizes); $overallSeqCount += $seqCount; # my $totalSize=`ave -col=2 $chromSizes | grep "^total" | awk '{printf "%d", \$NF}'`; $overallNucleotides += $totalSize; my $gapCount = gapStats($buildDir, $asmId); $overallGapCount += $gapCount; my $sciName = "notFound"; my $commonName = "notFound"; my $asmDate = "notFound"; my $itemsFound = 0; open (FH, "<$asmReport") or die "can not read $asmReport"; while (my $line = <FH>) { last if ($itemsFound > 5); chomp $line; @@ -289,60 +311,63 @@ $sciName = $line; $commonName =~ s/.*\(//; $commonName =~ s/\)//; $commonName = $betterName{$asmId} if (exists($betterName{$asmId})); $sciName =~ s/.*:\s+//; $sciName =~ s/\s+\(.*//; } } } close (FH); my $hubUrl = "https://hgdownload.soe.ucsc.edu/hubs/$accessionDir/$accessionId"; printf "<tr><td align=right>%d</td>\n", ++$asmCount; printf "<td align=center><a href='https://genome-test.gi.ucsc.edu/h/%s' target=_blank>%s<br>%s</a></td>\n", $accessionId, $commonName, $accessionId; foreach my $track (@trackList) { my $trackFile = "$buildDir/bbi/$asmId.$track"; + my $trackFb = "$buildDir/trackData/$track/fb.$asmId.$track.txt"; + my $runDir = "$buildDir/trackData/$track"; my ($itemCount, $percentCover); if ( "$track" eq "gc5Base" ) { $trackFile .= ".bw"; } else { $trackFile .= ".bb"; } if ( "$track" eq "rmsk") { my $rmskStats = "$buildDir/trackData/repeatMasker/$asmId.rmsk.stats"; if (! -s "${rmskStats}") { my $faOut = "$buildDir/trackData/repeatMasker/$asmId.sorted.fa.out.gz"; if ( -s "$faOut") { my $items = `zcat "$faOut" | wc -l`; chomp $items; $itemCount = commify($items); my $masked = `grep masked "$buildDir/trackData/repeatMasker/faSize.rmsk.txt" | awk '{print \$4}' | sed -e 's/%//;'`; chomp $masked; $percentCover = sprintf("%.2f %%", $masked); open (RS, ">$rmskStats") or die "can now write to $rmskStats"; printf RS "%s\t%s\n", $itemCount, $percentCover; close (RS); } else { $itemCount = "n/a"; $percentCover = "n/a"; } } else { ($itemCount, $percentCover) = split('\s+', `cat $rmskStats`); + $percentCover = sprintf("%.2f %%", $percentCover); chomp $percentCover; } } else { - ($itemCount, $percentCover) = oneTrackData($trackFile, $totalSize); + ($itemCount, $percentCover) = oneTrackData($asmId, $track, $trackFile, $totalSize, $trackFb, $runDir); } printf " <td align=right>%s<br>(%s)</td>\n", $itemCount, $percentCover; } printf "</tr>\n"; } } ############################################################################## ### main() ############################################################################## open (FH, "<$toolsDir/${commonNameOrder}") or die "can not read ${commonNameOrder}"; while (my $line = <FH>) { next if ($line =~ m/^#/); chomp $line;