475424b5d722b4ff761681f1d3bd91ad666fb2d6 angie Wed Apr 1 10:17:03 2020 -0700 For nested clades, add all clade samples to per-clade VCF. Also generate Newick (.nh) tree file and tweak sample name separator, in preparation for drawing the nextstrain tree in the VCF left label area. refs #25188 diff --git src/hg/utils/otto/nextstrainNcov/doUpdate.sh src/hg/utils/otto/nextstrainNcov/doUpdate.sh index cd735e1..299f077 100755 --- src/hg/utils/otto/nextstrainNcov/doUpdate.sh +++ src/hg/utils/otto/nextstrainNcov/doUpdate.sh @@ -1,65 +1,67 @@ #!/bin/bash set -beEu -o pipefail # Do not modify this script, modify the source tree copy: # kent/src/hg/utils/nextstrainNcov/doUpdate.sh ottoDir=/hive/data/outside/otto/nextstrainNcov chromSizes=/hive/data/genomes/wuhCor1/chrom.sizes gbdbDir=/gbdb/wuhCor1/nextstrain cd $ottoDir # The file is named .json, but is actually gzipped, so gunzip it. curl -s http://data.nextstrain.org/ncov.json | gunzip -c > ncov.json curl -s -I http://data.nextstrain.org/ncov.json \ | grep Last-Mod | sed -re 's/Last-Modified: //; s/\r//;' \ > ncov.json.date latestDate=$(cat ncov.json.date) oldDate=$(cat old.ncov.json.date) if [ $(date -d "$latestDate" +%s) -le $(date -d "$oldDate" +%s) ]; then exit 0 fi today=`date +%F` mkdir -p $today cp -p ncov.json $today mv ncov.json old.ncov.json mv ncov.json.date old.ncov.json.date runDir=$ottoDir/$today cd $runDir #Generate bed and VCF files $ottoDir/nextstrain.py # bgzip & tabix the VCF files bgzip -f nextstrainSamples.vcf tabix -p vcf nextstrainSamples.vcf.gz for clade in A1a A2 A2a A3 A6 A7 B B1 B2 B4; do bgzip -f nextstrainSamples$clade.vcf tabix -p vcf nextstrainSamples$clade.vcf.gz done # bigBed-ify the gene names and "clades" bedToBigBed -type=bed4 -tab -verbose=0 nextstrainGene.bed $chromSizes \ nextstrainGene.bb sort -k2n,2n nextstrainClade.bed > nextstrainClade.sorted.bed bedToBigBed -as=$ottoDir/nextstrainClade.as -type=bed12+7 -tab -verbose=0 \ nextstrainClade.sorted.bed $chromSizes \ nextstrainClade.bb # Archive mkdir -p $ottoDir/archive/$today cp -p $runDir/nextstrainGene.bb $runDir/nextstrainClade.bb \ $runDir/nextstrainSamples*.vcf.gz{,.tbi} \ + $runDir/nextstrain.nh \ + $runDir/ncov.json \ $ottoDir/archive/$today -cp -p $runDir/ncov.json $ottoDir/archive/$today # Install ln -sf $runDir/nextstrainGene.bb $runDir/nextstrainClade.bb \ $runDir/nextstrainSamples*.vcf.gz{,.tbi} \ + $runDir/nextstrain.nh \ $gbdbDir/ echo "Updated nextstrain/ncov `date` (ncov.json date $latestDate)"