1cec534c306475b701fb148763bc3edc021a0be5 kate Mon May 4 16:35:08 2020 -0700 Fix formatting error in track description. refs #25130 diff --git src/hg/makeDb/trackDb/human/gtexGeneV8.html src/hg/makeDb/trackDb/human/gtexGeneV8.html index 901a6d3..9cd4a10 100644 --- src/hg/makeDb/trackDb/human/gtexGeneV8.html +++ src/hg/makeDb/trackDb/human/gtexGeneV8.html @@ -49,58 +49,51 @@ NCI histopathology viewer</a>. The Qiagen PAXgene non-formalin tissue preservation product was used to stabilize tissue specimens without cross-linking biomolecules.</P> <P> RNA-seq was performed by the GTEx Laboratory, Data Analysis and Coordinating Center (LDACC) at the Broad Institute. The Illumina TruSeq protocol was used to create an unstranded polyA+ library sequenced on the Illumina HiSeq 2000 and HiSeq 2500 platforms to produce 76-bp paired end reads with a coverage goal of 50M (median achieved was ~82M total reads). </P> Sequence reads were aligned to the hg38/GRCh38 human genome using STAR v2.5.3a assisted by the GENCODE 26 transcriptome definition. The alignment pipeline is available <a target="_blank" href="https://github.com/broadinstitute/gtex-pipeline/tree/master/rnaseq">here</a>. </p> -<a> +<p> Gene annotations were produced using a custom isoform collapsing procedure that excluded retained intron and read through transcripts, merged overlapping exon intervals and then excluded exon intervals overlapping between genes. Gene expression levels in TPM were called via the RNA-SeQC tool (v1.1.9), after filtering for unique mapping, proper pairing, and exon overlap. For further method details, see the <a target="_blank" href="https://gtexportal.org/home/documentationPage#staticTexAnalysisMethods"> -GTEx Portal Documentation</a> page. +GTEx Portal Documentation</a> page.</p> <P> UCSC obtained the gene-level expression files, gene annotations and sample metadata from the GTEx Portal Download page. Median expression level in TPM was computed per gene/per tissue.</P> <H2>Subject and Sample Characteristics</H2> <P> The scientific goal of the GTEx project required that the donors and their biospecimen present with no evidence of disease. The tissue types collected were chosen based on their clinical significance, logistical feasibility and their relevance to the scientific goal of the project and the research community. -<!-- -Postmortem samples were collected from non-diseased donors with ages ranging from 20 to 79. 34.4% of donors were female and 65.6% male. -<div> <img border=1 src='/images/gtex/gtexSampleRin.V6.png'></div> -<p></p> -<div><img border=1 src='/images/gtex/gtexSampleAge.V6.png'></div></p> -<p> ---> Summary plots of GTEx sample characteristics are available at the <a target="_blank" href="https://gtexportal.org/home/tissueSummaryPage"> GTEx Portal Tissue Summary</a> page.</p> <h2>Data Access</h2> <p> The raw data for the GTEx Gene expression track can be accessed interactively through the <a href="hgTables?db=$db&hgta_track=$db&hgta_group=allTables&hgta_table=gtexGeneV8"> Table Browser</a> or <a href="hgIntegrator">Data Integrator</a>. Metadata can be found in the connected tables below. <ul> <li><strong><a href="hgTables?db=$db&hgta_track=$db&hgta_group=allTables&hgta_table=gtexGeneModelV8"> gtexGeneModelV8</a></strong> describes the gene names and coordinates in genePred format.</li>