ba48424667f579f95f96d49d1bc57467508d741f hiram Mon Apr 20 14:28:42 2020 -0700 mm10 canFam4 chainNet refs #25279 diff --git src/hg/makeDb/doc/mm10.txt src/hg/makeDb/doc/mm10.txt index 9d9538d..9cd4971 100644 --- src/hg/makeDb/doc/mm10.txt +++ src/hg/makeDb/doc/mm10.txt @@ -18576,16 +18576,83 @@ # real 48m44.604s cat fb.enhLutNer1.chainMm10Link.txt # 787727864 bases of 2413653822 (32.636%) in intersection cat fb.enhLutNer1.chainSynMm10Link.txt # 712950571 bases of 2413653822 (29.538%) in intersection time (doRecipBest.pl -load -workhorse=hgwdev enhLutNer1 mm10 \ -buildDir=`pwd`) > rbest.log 2>&1 & # real 167m45.490s cat fb.enhLutNer1.chainRBest.Mm10.txt # 734620004 bases of 2413653822 (30.436%) in intersection ############################################################################## +# LASTZ German shepard canFam4 (DONE - 2020-04-02 - Hiram) + # establish a screen to control this job + mkdir /hive/data/genomes/mm10/bed/lastzCanFam4.2020-04-02 + cd /hive/data/genomes/mm10/bed/lastzCanFam4.2020-04-02 + + printf '# German shepard canFam4 vs mouse +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.03/bin/lastz +BLASTZ_M=254 + +# TARGET: Mouse Mm10 +SEQ1_DIR=/hive/data/genomes/mm10/mm10.2bit +SEQ1_LEN=/hive/data/genomes/mm10/chrom.sizes +SEQ1_CHUNK=20000000 +SEQ1_LAP=10000 +SEQ1_LIMIT=40 +# QUERY: German shepard canFam4 +SEQ2_DIR=/hive/data/genomes/canFam4/canFam4.2bit +SEQ2_LEN=/hive/data/genomes/canFam4/chrom.sizes +SEQ2_CHUNK=10000000 +SEQ2_LIMIT=20 +SEQ2_LAP=0 + +BASE=/hive/data/genomes/mm10/bed/lastzCanFam4.2020-04-02 +TMPDIR=/dev/shm +' > DEF + + time (doBlastzChainNet.pl -verbose=2 `pwd`/DEF \ + -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > do.log 2>&1 & + # real 483m10.607s + + cat fb.mm10.chainCanFam4Link.txt + # 777883731 bases of 2652783500 (29.323%) in intersection + cat fb.mm10.chainSynCanFam4Link.txt + # 736602602 bases of 2652783500 (27.767%) in intersection + + + time (doRecipBest.pl -load -workhorse=hgwdev mm10 canFam4 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 219m16.168s + + cat fb.mm10.chainRBest.CanFam4.txt + # 741307883 bases of 2652783500 (27.945%) in intersection + + mkdir /hive/data/genomes/canFam4/bed/blastz.mm10.swap + cd /hive/data/genomes/canFam4/bed/blastz.mm10.swap + time (doBlastzChainNet.pl -verbose=2 \ + /hive/data/genomes/mm10/bed/lastzCanFam4.2020-04-02/DEF \ + -swap -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > swap.log 2>&1 & + # real 50m20.639s + + cat fb.canFam4.chainMm10Link.txt + # 772902855 bases of 2481941580 (31.141%) in intersection + cat fb.canFam4.chainSynMm10Link.txt + # 737924732 bases of 2481941580 (29.732%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev canFam4 mm10 \ + -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & + # real 173m38.016s + + cat fb.canFam4.chainRBest.Mm10.txt + # 740357755 bases of 2481941580 (29.830%) in intersection + +##############################################################################