46d4388aa7f2d939c4712d33053812d7ec1a7f97 hiram Wed Sep 23 09:55:39 2020 -0700 accomodate duplicate sequences refs #24396 diff --git src/hg/utils/automation/asmHubChromAlias.pl src/hg/utils/automation/asmHubChromAlias.pl index 142fca7..c8bfe1c 100755 --- src/hg/utils/automation/asmHubChromAlias.pl +++ src/hg/utils/automation/asmHubChromAlias.pl @@ -7,187 +7,212 @@ if ($argc != 1) { printf STDERR "usage: asmHubChromAlias.pl asmId > asmId.chromAlias.tab\n\n"; printf STDERR "where asmId is something like: GCF_000001735.4_TAIR10.1\n"; printf STDERR "Outputs a tab file suitable for processing with ixIxx to\n"; printf STDERR "create an index file to use in an assembly hub.\n"; printf STDERR "This command assumes it is in a work directory in the\n"; printf STDERR "assembly hub: .../asmId/trackData/chromAlias/\n"; printf STDERR "and .../asmId/downloads/ and .../asmId/sequence/ have\n"; printf STDERR "been created and processed for the hub build.\n"; exit 255; } my %ucscToRefSeq; # key is UCSC sequence name, value is RefSeq name my %ucscToGenbank; # key is UCSC sequence name, value is GenBank name my $ucscNames = 0; # == 1 if sequence is UCSC names, == 0 if NCBI names +my $dupCount = 0; my %aliasOut; # key is alias name, value is sequence name in this assembly sub showAlias() { printf "# sequenceName\talias names\n"; my %chromIndex; # key is sequence name in assembly, value # is a tab separated list of aliases foreach my $alias (sort keys %aliasOut) { my $name = $aliasOut{$alias}; -# printf "%s\t%s\n", $alias, $aliasOut{$alias}; + next if ($alias eq "na"); if (defined($chromIndex{$name})) { $chromIndex{$name} .= "\t" . $alias; } else { $chromIndex{$name} = $alias; } } foreach my $name (sort keys %chromIndex) { printf "%s\t%s\n", $name, $chromIndex{$name}; } } # given an alias and a sequence name, add to result or verify identical # to previous add sub addAlias($$) { my ($alias, $sequence) = @_; # do not need to add the sequence name itself return if ($alias eq $sequence); # already done, verify it is equivalent to previous request if (defined($aliasOut{$alias})) { if ($sequence ne $aliasOut{$alias}) { printf STDERR "ERROR: additional alias '%s:%s' does not match previous '%s'\n", $alias, $sequence, $aliasOut{$alias}; exit 255; } return; } $aliasOut{$alias} = $sequence; return; } -############################################################################## -########## experimental addVariations, not in use at this time ############### -############################################################################## -sub addVariations($$) { - my ($alias, $sequence) = @_; - addAlias($alias, $sequence); - return; - - addAlias(lc($alias), $sequence); # add lower case equivalent - my $noDotName = $alias; - $noDotName =~ s/\..*//; - addAlias($noDotName, $sequence); # add noDot name - addAlias(lc($noDotName), $sequence); # and lower case noDot nae - return; -} - my $asmId = shift; my $refSeq = 0; # == 0 for Genbank assembly, == 1 for RefSeq assembly $refSeq = 1 if ($asmId =~ m/^GCF/); my $twoBit = "../../$asmId.2bit"; my $sequenceCount = 0; - my %sequenceSizes; # key is sequence name, value is sequence size open (FH, "twoBitInfo $twoBit stdout|") or die "can not twoBitInfo $twoBit stdout"; while (my $line = <FH>) { chomp $line; my ($name, $size) = split('\s+', $line); $sequenceSizes{$name} = $size; ++$sequenceCount; } close (FH); my $nameCount = 0; -my %ncbiNames; # key is NCBI sequence name, value is 'chr' UCSC chromosome name -my %ucscNames; # key is 'chr' UCSC name, value is NCBI sequence name +my %ncbiToUcsc; # key is NCBI sequence name, value is 'chr' UCSC chromosome name +my %ucscToNcbi; # key is 'chr' UCSC name, value is NCBI sequence name open (FH, "cat ../../sequence/*.names|") or die "can not cat ../../sequence/*.names"; while (my $line = <FH>) { chomp $line; my ($ucscName, $seqName) = split('\s+', $line); - $ncbiNames{$seqName} = $ucscName; - $ucscNames{$ucscName} = $seqName; + $ncbiToUcsc{$seqName} = $ucscName; + $ucscToNcbi{$ucscName} = $seqName; ++$nameCount; $ucscNames = 1 if (defined($sequenceSizes{$ucscName})); if ($refSeq) { $ucscToRefSeq{$ucscName} = $seqName; } else { $ucscToGenbank{$ucscName} = $seqName; } } close (FH); +my $dupsNotFound = 0; +my $dupsList = "../../download/$asmId.dups.txt.gz"; +if ( -s "$dupsList" ) { + open (FH, "zcat $dupsList | awk '{print \$1, \$3}'|") or die "can not read $dupsList"; + while (my $line = <FH>) { + chomp $line; +# my ($dupAlias, $dupTarget) = split('\s+', $line); + my ($dupTarget, $dupAlias) = split('\s+', $line); + if ($ucscNames) { + if (!defined($ncbiToUcsc{$dupTarget})) { + printf STDERR "# ERROR: can not find dupTarget: $dupTarget in ncbiToUcsc for dupAlias: $dupAlias\n"; + $dupsNotFound += 1; + } else { + addAlias($dupAlias, $ncbiToUcsc{$dupTarget}); + } + } elsif (defined($ucscToNcbi{$dupTarget})) { + addAlias($dupAlias, $ucscToNcbi{$dupTarget}); + } else { + printf STDERR "# ERROR: can not find duplicate name $dupAlias for sequence $dupTarget\n"; + $dupsNotFound += 1; + } + ++$dupCount; + } + close (FH); +} + +if ($dupsNotFound > 0) { + printf STDERR "ERROR: can not find %d duplicate names\n", $dupsNotFound; + exit 255; +} + if ($sequenceCount != $nameCount) { printf STDERR "ERROR: do not find the same name count in sequence vs. names files\n"; - printf STDERR "sequenceCount %d != %d names count\n", $sequenceCount, $nameCount; + printf STDERR "sequenceCount %d != %d names count - %d duplicates\n", $sequenceCount, $nameCount, $dupCount; exit 255; } -printf STDERR "# initial set with UCSC names\n"; +printf STDERR "# initial set of %d UCSC names\n", $sequenceCount; ### first set of names is the UCSC to NCBI sequence names foreach my $sequence (sort keys %sequenceSizes) { my $seqName = $sequence; my $alias = $sequence; -# addVariations($seqName, $alias); # obsolete identical usage if ($ucscNames) { - $alias = $ucscNames{$seqName}; + $alias = $ucscToNcbi{$seqName}; } else { - $alias = $ncbiNames{$seqName}; + $alias = $ncbiToUcsc{$seqName}; } if (!defined($alias)) { printf STDERR "ERROR: can not find alias name for '%s' sequence\n", $seqName; exit 255; } - addVariations($alias, $seqName); + addAlias($alias, $seqName); } # foreach my $sequence (sort keys %chrNames) { # printf "%s\t%s\n", $chrNames{$sequence}, $sequence; # } ### next set of names are the equivalents declared by NCBI ### if they exist my %chr2acc; # key is sequence name, value is NCBI chromosome name my $asmStructCount = `ls ../../download/${asmId}_assembly_structure/*/*/chr2acc 2> /dev/null | wc -l`; chomp $asmStructCount; if ( $asmStructCount > 0 ) { printf STDERR "# second name set processing chr2acc files\n"; open (FH, "grep -h -v '^#' ../../download/${asmId}_assembly_structure/*/*/chr2acc|") or die "can not grep chr2acc files"; while (my $line = <FH>) { chomp $line; my ($alias, $seqName) = split('\s+', $line); $chr2acc{$seqName} = $alias; if ($ucscNames) { - $seqName = $ncbiNames{$seqName}; + $seqName = $ncbiToUcsc{$seqName}; } - addVariations($alias, $seqName); + addAlias($alias, $seqName); } close (FH); } # foreach my $sequence (sort keys %chr2acc) { # printf "%s\t%s\n", $chr2acc{$sequence}, $sequence; # } my %gbk2acc; # key is refseq name, value is genbank accession printf STDERR "# third set processing assembly_report\n"; # column 5 is the GenBank-Accn, column 7 is the RefSeq-Accn open (FH, "grep -v '^#' ../../download/${asmId}_assembly_report.txt | cut -d\$'\t' -f5,7|") or die "can not grep assembly_report"; while (my $line = <FH>) { chomp $line; my ($gbkName, $refSeqName) = split('\s+', $line); - next if ($refSeqName eq "na"); # may not be any RefSeq names + next if ($refSeqName eq "na"); # may not be any RefSeq name + next if ($gbkName eq "na"); # may not be any GenBank name $gbk2acc{$gbkName} = $refSeqName; if ($refSeq) { my $seqName = $refSeqName; if ($ucscNames) { - $seqName = $ncbiNames{$seqName}; + $seqName = $ncbiToUcsc{$seqName}; + } + if (!defined($seqName)) { + if (defined($aliasOut{$refSeqName})) { + $seqName = $aliasOut{$refSeqName}; } - addVariations($gbkName, $seqName); + } + addAlias($gbkName, $seqName); } else { my $seqName = $gbkName; if ($ucscNames) { - $seqName = $ncbiNames{$seqName}; + $seqName = $ncbiToUcsc{$seqName}; + } + if (!defined($seqName)) { + if (defined($aliasOut{$gbkName})) { + $seqName = $aliasOut{$gbkName}; + } } - addVariations($refSeqName, $seqName); + addAlias($refSeqName, $seqName); } } close (FH); showAlias();