99599c7130790107ff0de9f043930da6aa7fddf1 angie Mon Nov 16 16:35:58 2020 -0800 Scripts for automating SARS-CoV-2 Phylogeny tracks (refs #26530): fetching sequences and metadata from several public sources, mapping GISAID IDs to public seq IDs, downloading the latest release of the phylogenetic tree from github.com/roblanf/sarscov2phylo/ , making VCFs from GISAID and public sequences, and using github.com/yatisht/usher to resolve ambiguous alleles, make protobuf files for hgPhyloPlace, and add public sequences that have not been mapped to GISAID sequences to the sarscov2phylo tree for a comprehensive public tree+VCF. This is still not fully otto-mated because certain crucial inputs like GISAID sequences still must be downloaded using a web browser, but the goal is to automate as much as possible and maybe someday have it fully cron-driven. There are two main top-level scripts which call other scripts, which may in turn call scripts, in this hierarchy: updateIdMapping.sh getCogUk.sh getNcbi.sh searchAllSarsCov2BioSample.sh bioSampleIdToText.sh bioSampleTextToTab.pl gbMetadataAddBioSample.pl fixNcbiFastaNames.pl updateSarsCov2Phylo.sh getRelease.sh processRelease.sh cladeLineageColors.pl mapPublic.sh extractUnmappedPublic.sh addUnmappedPublic.sh many of the above: util.sh publicCredits.sh will hopefully be folded into updateSarsCov2Phylo.sh when I figure out how to automate fetching of author/institution metadata from NCBI and COG-UK. diff --git src/hg/utils/otto/sarscov2phylo/getCogUk.sh src/hg/utils/otto/sarscov2phylo/getCogUk.sh new file mode 100755 index 0000000..d2a2e2c --- /dev/null +++ src/hg/utils/otto/sarscov2phylo/getCogUk.sh @@ -0,0 +1,27 @@ +#!/bin/bash + +set -beEu -o pipefail + +# Download latest COG-UK fasta and metadata; update $ottoDir/cogUk.latest link. + +scriptDir=$(dirname "${BASH_SOURCE[0]}") +source $scriptDir/util.sh + +today=$(date +%F) + +ottoDir=/hive/data/outside/otto/sarscov2phylo +cogUrlBase=https://cog-uk.s3.climb.ac.uk/phylogenetics/latest + +mkdir -p $ottoDir/cogUk.$today +cd $ottoDir/cogUk.$today + +wget -q $cogUrlBase/cog_all.fasta +wget -q $cogUrlBase/cog_metadata.csv + +xz -f -T 50 cog_all.fasta + +tail -n +2 cog_metadata.csv \ +| awk -F, '{print $1 "\t" $4;}' | sort > cogUkToDate + +rm -f $ottoDir/cogUk.latest +ln -s cogUk.$today $ottoDir/cogUk.latest