fe8105565c585384c9d79752eb2f397d841acc62 hiram Tue Mar 2 11:01:19 2021 -0800 wrapping up lastz runs to xenTro10 refs #24693 diff --git src/hg/makeDb/doc/hg38/lastzRuns.txt src/hg/makeDb/doc/hg38/lastzRuns.txt index b2be5fc..8f4c106 100644 --- src/hg/makeDb/doc/hg38/lastzRuns.txt +++ src/hg/makeDb/doc/hg38/lastzRuns.txt @@ -12987,16 +12987,99 @@ # real 74m20.215s sed -e 's/^/ # /;' fb.rn7.chainHg38Link.txt # 928866703 bases of 2626580772 (35.364%) in intersection sed -e 's/^/ # /;' fb.rn7.chainSynHg38Link.txt # 879484562 bases of 2626580772 (33.484%) in intersection time (doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ rn7 hg38) > rbest.log 2>&1 & # real 373m6.610s sed -e 's/^/ # /;' fb.rn7.chainRBest.Hg38.txt # 885516265 bases of 2626580772 (33.714%) in intersection -######################################################################### +############################################################################# +# LASTZ human/hg38 vs. X. tropicalis/xenTro10 - (DONE - 2021-02-22 - Hiram) + mkdir /hive/data/genomes/hg38/bed/lastzXenTro10.2021-02-22 + cd /hive/data/genomes/hg38/bed/lastzXenTro10.2021-02-22 + + printf '# human vs X. tropicalis +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.03/bin/lastz +BLASTZ_O=400 +BLASTZ_E=30 +BLASTZ_Y=3400 +BLASTZ_L=6000 +BLASTZ_K=2200 +BLASTZ_M=50 +BLASTZ_Q=/hive/data/staging/data/blastz/HoxD55.q +# A C G T +# 91 -90 -25 -100 +# -90 100 -100 -25 +# -25 -100 100 -90 +# -100 -25 -90 91 + +# TARGET: human hg38 +SEQ1_DIR=/hive/data/genomes/hg38/hg38.2bit +SEQ1_LEN=/hive/data/genomes/hg38/chrom.sizes +SEQ1_CTGDIR=/hive/data/genomes/hg38/hg38.contigs.2bit +SEQ1_CTGLEN=/hive/data/genomes/hg38/hg38.contigs.chrom.sizes +SEQ1_LIFT=/hive/data/genomes/hg38/jkStuff/hg38.contigs.lift +SEQ1_CHUNK=40000000 +SEQ1_LIMIT=10 +SEQ1_LAP=10000 + +# QUERY: X. tropicalis xenTro10 +SEQ2_DIR=/hive/data/genomes/xenTro10/xenTro10.2bit +SEQ2_LEN=/hive/data/genomes/xenTro10/chrom.sizes +SEQ2_CHUNK=20000000 +SEQ2_LIMIT=20 +SEQ2_LAP=0 + +BASE=/hive/data/genomes/hg38/bed/lastzXenTro10.2021-02-22 +TMPDIR=/dev/shm +' > DEF + + time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 \ + -chainMinScore=5000 -chainLinearGap=loose \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -syntenicNet) > do.log 2>&1 & + # real 805m18.879s + + sed -e 's/^/ # /;' fb.hg38.chainXenTro10Link.txt + # 146412457 bases of 3110768607 (4.707%) in intersection + sed -e 's/^/ # /;' fb.hg38.chainSynXenTro10Link.txt + # 41291684 bases of 3110768607 (1.327%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` hg38 xenTro10) \ + > rbest.log 2>&1 & + # real 435m37.210s + + sed -e 's/^/ # /;' fb.hg38.chainRBest.XenTro10.txt + # 73679844 bases of 3110768607 (2.369%) in intersection + + # and for the swap: + mkdir /hive/data/genomes/xenTro10/bed/blastz.hg38.swap + cd /hive/data/genomes/xenTro10/bed/blastz.hg38.swap + + time (doBlastzChainNet.pl -verbose=2 \ + /hive/data/genomes/hg38/bed/lastzXenTro10.2021-02-22/DEF \ + -swap -chainMinScore=5000 -chainLinearGap=loose \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -syntenicNet) > swap.log 2>&1 + # real 79m35.244s + + sed -e 's/^/ # /;' fb.xenTro10.chainHg38Link.txt + # 150875559 bases of 1448461978 (10.416%) in intersection + + sed -e 's/^/ # /;' fb.xenTro10.chainSynHg38Link.txt + # 40317510 bases of 1448461978 (2.783%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` xenTro10 hg38) \ + > rbest.log 2>&1 +XXX - running - Tue Mar 2 10:52:22 PST 2021 + # real 749m24.229s + + sed -e 's/^/ # /;' fb.xenTro10.chainRBest.Hg38.txt + +#############################################################################