2eb7675b61bc79306e6ec54839140d2b40b31bf5 hiram Mon May 24 12:19:29 2021 -0700 canFam6 dog Tasha browser completed refs #27546 diff --git src/hg/makeDb/doc/hg38/lastzRuns.txt src/hg/makeDb/doc/hg38/lastzRuns.txt index c400232..d5d4d62 100644 --- src/hg/makeDb/doc/hg38/lastzRuns.txt +++ src/hg/makeDb/doc/hg38/lastzRuns.txt @@ -13170,15 +13170,89 @@ time (doRecipBest.pl -trackHub -load -workhorse=hgwdev -buildDir=`pwd` \ -target2Bit="/hive/data/genomes/asmHubs/$gcPath/${query}/${query}.2bit" \ -targetSizes="/hive/data/genomes/asmHubs/$gcPath/${query}/${query}.chrom.sizes.txt" \ $query $target) >> rbest.log 2>&1 grep -w real rbest.log | sed -e 's/^/ # /;' # real 463m23.874s sed -e 's/^/ # /;' fb.${query}.chainRBest.${Target}.txt # 1281096559 bases of 2869914396 (44.639%) in intersection # Complete run time for all the swap operation: # real 554m2.489s ############################################################################## +# LASTZ human/hg38 vs. boxer/Tasha/canFam6 +# (DONE - 2021-05-17 - Hiram) + + mkdir /hive/data/genomes/hg38/bed/lastzCanFam6.2021-05-17 + cd /hive/data/genomes/hg38/bed/lastzCanFam6.2021-05-17 + + printf '# human vs. dog (boxer Tasha) +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.03/bin/lastz +BLASTZ_M=254 + +# TARGET: human hg38 +SEQ1_DIR=/hive/data/genomes/hg38/hg38.2bit +SEQ1_LEN=/hive/data/genomes/hg38/chrom.sizes +SEQ1_CTGDIR=/hive/data/genomes/hg38/hg38.contigs.2bit +SEQ1_CTGLEN=/hive/data/genomes/hg38/hg38.contigs.chrom.sizes +SEQ1_LIFT=/hive/data/genomes/hg38/jkStuff/hg38.contigs.lift +SEQ1_CHUNK=40000000 +SEQ1_LIMIT=20 +SEQ1_LAP=10000 + +# QUERY: boxer Tasha/canFam6 +SEQ2_DIR=/hive/data/genomes/canFam6/canFam6.2bit +SEQ2_LEN=/hive/data/genomes/canFam6/chrom.sizes +SEQ2_CHUNK=20000000 +SEQ2_LIMIT=20 +SEQ2_LAP=0 + +BASE=/hive/data/genomes/hg38/bed/lastzCanFam6.2021-05-17 +TMPDIR=/dev/shm +' > DEF + + time (~/kent/src/hg/utils/automation/doBlastzChainNet.pl `pwd`/DEF \ + -verbose=2 -chainMinScore=3000 -chainLinearGap=medium \ + -workhorse=hgwdev -smallClusterHub=hgwdev -bigClusterHub=ku \ + -syntenicNet) > do.log 2>&1 + # real 589m47.086s + + sed -e 's/^/ # /;' fb.hg38.chainCanFam6Link.txt + # 1529157815 bases of 3110768607 (49.157%) in intersection + + sed -e 's/^/ # /;' fb.hg38.chainSynCanFam6Link.txt + # 1468477892 bases of 3110768607 (47.206%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ + hg38 canFam6) > rbest.log 2>&1 & + # real 389m44.734s + + sed -e 's/^/ # /;' fb.hg38.chainRBest.CanFam6.txt + # 1406453581 bases of 3110768607 (45.212%) in intersection + + # and for the swap: + mkdir /hive/data/genomes/canFam6/bed/blastz.hg38.swap + cd /hive/data/genomes/canFam6/bed/blastz.hg38.swap + + time (doBlastzChainNet.pl -verbose=2 \ + /hive/data/genomes/hg38/bed/lastzCanFam6.2021-05-17/DEF \ + -swap -chainMinScore=3000 -chainLinearGap=medium \ + -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ + -syntenicNet) > swap.log 2>&1 + # real 84m5.076s + + sed -e 's/^/ # /;' fb.canFam6.chainHg38Link.txt + # 1435515352 bases of 2312743346 (62.070%) in intersection + sed -e 's/^/ # /;' fb.canFam6.chainSynHg38Link.txt + # 1400962536 bases of 2312743346 (60.576%) in intersection + + time (doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ + canFam6 hg38) > rbest.log 2>&1 & + # real 270m7.152s + + sed -e 's/^/ # /;' fb.canFam6.chainRBest.Hg38.txt + # 1406634909 bases of 2312743346 (60.821%) in intersection + +############################################################################