78a15f21fb3bfad23eac7217e6d831810558304e angie Thu Sep 2 17:50:15 2021 -0700 Adding note about COG-UK credits in BioSample text (although I should repeat with the new biosample.jsonl file). diff --git src/hg/utils/otto/sarscov2phylo/publicCredits.sh src/hg/utils/otto/sarscov2phylo/publicCredits.sh index e50d582..51c367f 100755 --- src/hg/utils/otto/sarscov2phylo/publicCredits.sh +++ src/hg/utils/otto/sarscov2phylo/publicCredits.sh @@ -1,70 +1,84 @@ #!/bin/bash set -beEu -x -o pipefail # Do not modify this script, modify the source tree copy: # kent/src/hg/utils/otto/sarscov2phylo/publicCredits.sh usage() { echo "usage: $0" } if [ $# != 0 ]; then usage exit 1 fi cncbMetadata=/hive/data/outside/otto/sarscov2phylo/cncb.latest/cncb.metadata.tsv ncbiMetadata=ncbi.authors.20-11-13.csv #*** TODO AUTOMATE ME # * https://www.ncbi.nlm.nih.gov/labs/virus/vssi/#/virus?SeqType_s=Nucleotide&VirusLineage_ss=SARS-CoV-2,%20taxid:2697049 # * Download button # * Current table view result --> CSV format, Next button # * Download all records, Next button # * Select Accession and Authors [no labs options unfortunately] # * Download button, save as ncbi.authors.date.csv #*** TODO AUTOMATE ME # * https://www.ebi.ac.uk/ena/browser/view/PRJEB37886 # * select columns center_name, sample_accession, sample_alias # * Download report: TSV # * file saved to filereport_read_run_PRJEB37886_tsv.2020-11-3.txt (extra first column, run_accession) # 2021-01-08: the project seems to have 133312 sequences, but the download file is cut off # after 100,000. It would probably be more efficient to just identify all of the country/center # strings in strain names, and ask COG-UK how to attribute sequences from each one. cogUkMetadata=filereport_read_run_PRJEB37886_tsv.2020-11-13.txt +# Actually BioSample has this for COG-UK: +# xzcat $ncbiDir/all.bioSample.tab.xz \ +# | grep COG-UK \ +# | sed -re 's@COG-UK/@@; s/(\t202[01])[0-9-]+/\1/;' \ +# | tawk '{print $8 "/" $3 "/" $4, $5;}' \ +# | sort > cogToLab +# -- although some entries break it down like this: +# Wales/QEUH-B2C4CB/2020 Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit +# -- well, I guess just that one: +# g : cogToLab | cut -f 2 | sort | uniq -c | sort -nr | less +# 38415 Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit + + + # Author credits file... strip GenBank version numbers because NCBI metadata doesn't have those cut -f 2 treeToPublic \ | cut -d \| -f 2 \ | sed -re 's/^([A-Z][A-Z][0-9]{6})\.[0-9]/\1/;' \ | sort > publicIdsInTree tail -n+2 $cncbMetadata \ | cut -f 2,12,14 \ | grep -v ^EPI_ISL_ \ | egrep -v '^[A-Z][A-Z][0-9]{6}' \ | sed -e 's/"//g; s/$/\tn\/a/;' \ > cncb.credits tail -n+2 $ncbiMetadata \ | csvToTab \ | tawk '{print $1, "n/a", "n/a", $2;}' \ > ncbi.credits tail -n+2 $cogUkMetadata \ | tawk '{print $4, $3, $3, "COVID-19 Genomics UK Consortium";}' \ | sed -e 's@^COG-UK/@@;' \ | sort -u \ > cogUk.credits.partialIds grep / publicIdsInTree \ | awk -F/ '{print $2 "\t" $0;}' \ | sort \ > cogUk.partialToFull join -a 2 -e "n/a" -t$'\t' -o 2.2,1.2,1.3,1.4 cogUk.credits.partialIds cogUk.partialToFull \ | tawk '{ if ($4 == "n/a") { $4 = "COVID-19 Genomics UK Consortium"; } print; }' \ > cogUk.credits /bin/echo -e "accession\toriginating_lab\tsubmitting_lab\tauthors" > acknowledgements.tsv #*** THIS IS just for the subset... we need a full acknowledgements file too. grep -Fwf publicIdsInTree cncb.credits >> acknowledgements.tsv grep -Fwf publicIdsInTree ncbi.credits >> acknowledgements.tsv grep -Fwf publicIdsInTree cogUk.credits >> acknowledgements.tsv gzip acknowledgements.tsv