Tracks labeled as Probes (P) indicate the footprint of the oligonucleotide probes mapped to the human genome. This is the technically relevant targeted region by the assay. However, the sequenced region will be bigger than this since flanking sequences are sequenced as well. Tracks labeled as Target Regions (T) indicate the genomic regions targeted by the -assay. This is the biologically relevant target region. It's not granted that all targeted regions -will be sequenced perfectly, it might be some capture bias on certain locations. The Target +assay. This is the biologically relevant target region. Not all targeted regions +will necessarily be sequenced perfectly; there might be some capture bias at certain locations. +The Target Regions are those normally used for coverage analysis.
The capture of the genomic regions of interest using in-solution capture, is achieved through the hybridization of a set of probes (oligonucleotides) with a sample of fragmented genomic DNA in a solution environment. The probes hybridize selectively to the genomic regions of interest which, after a process of exclusion of the non-selective DNA material, can be pulled down and -sequenced enabling selective DNA sequencing of the genomic regions (e.g. exons) of interest. +sequenced, enabling selective DNA sequencing of the genomic regions of interest (e.g., exons). In-solution capture sequencing is a sensitive method to detect single nucleotide variants, insertions and deletions, and copy number variations.
| Kit | @@ -271,22 +272,23 @@ found in the Table Schema page for each subtrack after "Primary Table:".
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