1054d4e3c1d6bdb3d2799860d0eca70dfd35447c hiram Mon Oct 4 10:27:27 2021 -0700 mm39 vs. hg19 lastz/chain/net per Brian request no redmine diff --git src/hg/makeDb/doc/mm39/lastzRuns.txt src/hg/makeDb/doc/mm39/lastzRuns.txt index dc97846..3b7f56f 100644 --- src/hg/makeDb/doc/mm39/lastzRuns.txt +++ src/hg/makeDb/doc/mm39/lastzRuns.txt @@ -2226,15 +2226,93 @@ # real 45m15.626s sed -e 's/^/ # /;' fb.canFam6.chainMm39Link.txt # 749339105 bases of 2312743346 (32.400%) in intersection sed -e 's/^/ # /;' fb.canFam6.chainSynMm39Link.txt # 721148735 bases of 2312743346 (31.182%) in intersection time (doRecipBest.pl -load -workhorse=hgwdev canFam6 mm39 \ -buildDir=`pwd` -workhorse=hgwdev) > rbest.log 2>&1 & # real 204m49.212s sed -e 's/^/ # /;' fb.canFam6.chainRBest.Mm39.txt # 730973192 bases of 2312743346 (31.606%) in intersection ############################################################################## +# LASTZ human hg19 (DONE - 2021-09-30 - Hiram) + +# should be able to run this from anywhere, this time it was run from: + cd /hive/data/genomes/mm39/bed + + time (~/kent/src/hg/utils/automation/pairLastz.sh mm39 hg19 mammal primate) \ + > mm39.hg19.log 2>&1 + + # this command outputs this makeDoc text: + + cat lastz.hg19/makeDoc.txt + +############################################################################## +# LASTZ Mouse Mm39 vs. Human Hg19 (DONE - 2021-09-30 - Hiram) + mkdir /hive/data/genomes/mm39/bed/lastzHg19.2021-09-30 + cd /hive/data/genomes/mm39/bed/lastzHg19.2021-09-30 + + printf '# Human Hg19 vs. Mouse Mm39 +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.03/bin/lastz + +# TARGET: Mouse Mm39 +SEQ1_DIR=/hive/data/genomes/mm39/mm39.2bit +SEQ1_LEN=/hive/data/genomes/mm39/chrom.sizes +SEQ1_CHUNK=20000000 +SEQ1_LAP=10000 +SEQ1_LIMIT=40 + +# QUERY: Human Hg19 +SEQ2_DIR=/hive/data/genomes/hg19/hg19.2bit +SEQ2_LEN=/hive/data/genomes/hg19/chrom.sizes +SEQ2_CHUNK=20000000 +SEQ2_LAP=0 +SEQ2_LIMIT=100 + +BASE=/hive/data/genomes/mm39/bed/lastzHg19.2021-09-30 +TMPDIR=/dev/shm + +' > DEF + + time (~/kent/src/hg/utils/automation/doBlastzChainNet.pl -verbose=2 `pwd`/DEF -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=hgwdev -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > do.log 2>&1 + grep -w real do.log | sed -e 's/^/ # /;' + # real 474m8.118s + # # real 474m8.118s + # # real 370m29.622s + # real 844m37.788s + + sed -e 's/^/ # /;' fb.mm39.chainHg19Link.txt + # 938444606 bases of 2654624157 (35.351%) in intersection + + ### and for the swap + + cd /hive/data/genomes/hg19/bed/blastz.mm39.swap + + time (~/kent/src/hg/utils/automation/doBlastzChainNet.pl -swap -verbose=2 \ + /hive/data/genomes/mm39/bed/lastzHg19.2021-09-30/DEF -swapDir=`pwd` \ + -syntenicNet -workhorse=hgwdev -smallClusterHub=hgwdev -bigClusterHub=ku \ + -chainMinScore=3000 -chainLinearGap=medium) > swap.log 2>&1 + + grep -w real swap.log | sed -e 's/^/ # /;' + # real 57m17.328s + + sed -e 's/^/ # /;' fb.hg19.chainMm39Link.txt + # 969322683 bases of 2991710746 (32.400%) in intersection + sed -e 's/^/ # /;' fb.hg19.chainSynMm39Link.txt + # 921405754 bases of 2991710746 (30.799%) in intersection + + time (~/kent/src/hg/utils/automation/doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ + hg19 mm39) > rbest.log 2>&1 + + grep -w real rbest.log | sed -e 's/^/ # /;' + # real 299m2.449s + + sed -e 's/^/ # /;' fb.hg19.chainRBest.Mm39.txt + # 892863094 bases of 2991710746 (29.845%) in intersection + +##############################################################################