90555f803fa25829e2bf91e2faa913433999896f hiram Tue Apr 5 11:16:39 2022 -0700 new style chromAlias refs #29109 diff --git src/hg/utils/automation/asmHubChromAlias.pl src/hg/utils/automation/asmHubChromAlias.pl index 798f776..99c7af2 100755 --- src/hg/utils/automation/asmHubChromAlias.pl +++ src/hg/utils/automation/asmHubChromAlias.pl @@ -1,329 +1,373 @@ #!/usr/bin/env perl use strict; use warnings; my $argc = scalar(@ARGV); if ($argc != 1) { printf STDERR "usage: asmHubChromAlias.pl asmId > asmId.chromAlias.tab\n\n"; printf STDERR "where asmId is something like: GCF_000001735.4_TAIR10.1\n"; printf STDERR "Outputs a tab file suitable for processing with ixIxx to\n"; printf STDERR "create an index file to use in an assembly hub.\n"; printf STDERR "This command assumes it is in a work directory in the\n"; printf STDERR "assembly hub: .../asmId/trackData/chromAlias/\n"; printf STDERR "and .../asmId/downloads/ and .../asmId/sequence/ have\n"; printf STDERR "been created and processed for the hub build.\n"; exit 255; } my %ucscToRefSeq; # key is UCSC sequence name, value is RefSeq name my %ucscToGenbank; # key is UCSC sequence name, value is GenBank name my $ucscNames = 0; # == 1 if sequence is UCSC names, == 0 if NCBI names my $dupCount = 0; my %dupToSequence; # key is duplicate name, value is target sequence name # to manage duplicates coming in from assembly report my $sequenceCount = 0; my %sequenceSizes; # key is sequence name, value is sequence size my $asmId = shift; my %aliasOut; # key is source name, value is a hash pointer where # key is alias name and value is chrom name # typical source names: ucsc, refseq, genbank, ensembl, assembly, ncbi # the argument is the name of the 'native' source name # where 'native' are the names in the assembly itself, thus the # assembly names. The other sources are aliases to that. sub showAlias($) { my ($native) = @_; # second line is the column headers printf "# %s", $native; foreach my $source (sort keys %aliasOut) { printf "\t%s", $source if ($source ne $native); } printf "\n"; # need to account for all sequence names for all sources # create a list of sequence names for each source that does *not* have # an alias my %noAlias; # key is source, value is a hash pointer with # a key of seqName value 1 for those seqNames without alias foreach my $source (sort keys %aliasOut) { next if ($source eq $native); # don't need to do this for native my %seqDone; # key is seqName found in alias, value is 1 my $hashPtr = $aliasOut{$source}; foreach my $alias (sort keys %$hashPtr) { $seqDone{$hashPtr->{$alias}} = 1; } foreach my $sequence (sort keys %sequenceSizes) { next if (defined($seqDone{$sequence})); if (!defined($noAlias{$source})) { my %h; $noAlias{$source} = \%h; } $hashPtr = $noAlias{$source}; $hashPtr->{$sequence} = 1; } } # foreach my $source (sort keys %aliasOut) my %chromIndex; # key is sequence seqName in assembly, value # is a tab separated list of aliases, in order by source foreach my $source (sort keys %aliasOut) { next if ($source eq $native); my $hashPtr = $aliasOut{$source}; foreach my $alias (sort keys %$hashPtr) { my $seqName = $hashPtr->{$alias}; $alias = "" if ($alias eq "na"); if (defined($chromIndex{$seqName})) { $chromIndex{$seqName} .= "\t" . $alias; } else { $chromIndex{$seqName} = $alias; } } # catch up for those sequence names with no alias in this source if (defined($noAlias{$source})) { $hashPtr = $noAlias{$source}; foreach my $sequence (sort keys %sequenceSizes) { next if (!defined($hashPtr->{$sequence})); if (defined($chromIndex{$sequence})) { $chromIndex{$sequence} .= "\t" . ""; } else { $chromIndex{$sequence} = ""; } } } } foreach my $seqName (sort keys %chromIndex) { printf "%s\t%s\n", $seqName, $chromIndex{$seqName}; } } # given an alias and a sequence name, add to result or verify identical # to previous add sub addAlias($$$) { my ($source, $alias, $sequence) = @_; + if ($alias eq "na") { + return; + } + if ($sequence eq "na") { + return; + } # do not need to add the sequence name itself return if ($alias eq $sequence); if (!defined($aliasOut{$source})) { my %h; # hash: key: alias name, value 'native' chrom name $aliasOut{$source} = \%h; # printf STDERR "# creating aliasOut{'%s'}\n", $source; } my $hashPtr = $aliasOut{$source}; # already done, verify it is equivalent to previous request if (defined($hashPtr->{$alias})) { if ($sequence ne $hashPtr->{$alias}) { printf STDERR "ERROR: additional alias '%s:%s' does not match previous '%s'\n", $alias, $sequence, $hashPtr->{$alias}; exit 255; } return; } $hashPtr->{$alias} = $sequence; return; } # asmSource - is this a genbank or refseq assembly my $asmSource = "genbank"; my $isRefSeq = 0; # == 0 for Genbank assembly, == 1 for RefSeq assembly if ($asmId =~ m/^GCF/) { # printf STDERR "# processing a RefSeq assembly\n"; $isRefSeq = 1; $asmSource = "refseq"; } else { # printf STDERR "# processing a GenBank assembly\n"; } my $twoBit = "../../$asmId.2bit"; open (FH, "twoBitInfo $twoBit stdout|") or die "can not twoBitInfo $twoBit stdout"; while (my $line = <FH>) { chomp $line; my ($name, $size) = split('\s+', $line); $sequenceSizes{$name} = $size; ++$sequenceCount; } close (FH); # printf STDERR "# counted %d sequence names in the twoBit file\n", $sequenceCount; my $nameCount = 0; my %ncbiToUcsc; # key is NCBI sequence name, value is 'chr' UCSC chromosome name my %ucscToNcbi; # key is 'chr' UCSC name, value is NCBI sequence name open (FH, "cat ../../sequence/*.names|") or die "can not cat ../../sequence/*.names"; while (my $line = <FH>) { chomp $line; my ($ucscName, $seqName) = split('\s+', $line); $ncbiToUcsc{$seqName} = $ucscName; $ucscToNcbi{$ucscName} = $seqName; ++$nameCount; $ucscNames = 1 if (defined($sequenceSizes{$ucscName})); if ($isRefSeq) { $ucscToRefSeq{$ucscName} = $seqName; } else { $ucscToGenbank{$ucscName} = $seqName; } } close (FH); -# when not a UCSC named assembly, add the UCSC names as aliases -if (! $ucscNames) { - if ($isRefSeq) { - foreach my $ucscName (sort keys %ucscToRefSeq) { - addAlias("ucsc", $ucscName, $ucscToRefSeq{$ucscName}); - } - } else { - foreach my $ucscName (sort keys %ucscToGenbank) { - addAlias("ucsc", $ucscName, $ucscToGenbank{$ucscName}); - } - } -} - my $dupsNotFound = 0; my $dupsList = "../../download/$asmId.dups.txt.gz"; if ( -s "$dupsList" ) { open (FH, "zcat $dupsList | awk '{print \$1, \$3}'|") or die "can not read $dupsList"; while (my $line = <FH>) { chomp $line; my ($dupAlias, $dupTarget) = split('\s+', $line); $dupToSequence{$dupAlias} = $dupTarget; if ($ucscNames) { if (!defined($ncbiToUcsc{$dupTarget})) { printf STDERR "# ERROR: can not find dupTarget: $dupTarget in ncbiToUcsc for dupAlias: $dupAlias\n"; $dupsNotFound += 1; } else { - addAlias("ucsc", $dupAlias, $ncbiToUcsc{$dupTarget}); + printf STDERR "# addAlias(\"ucsc\", $dupAlias, $ncbiToUcsc{$dupTarget});\n"; } } else { - addAlias($asmSource, $dupAlias, $dupTarget); + printf STDERR "# addAlias($asmSource, $dupAlias, $dupTarget);\n"; } printf STDERR "# adding duplicate alias %s\n", $dupAlias; ++$dupCount; } close (FH); } if ($dupsNotFound > 0) { printf STDERR "ERROR: can not find %d duplicate names\n", $dupsNotFound; exit 255; } +if ($dupCount > 0) { + printf STDERR "# processed %d duplicates\n", $dupCount; +} + +# when not a UCSC named assembly, add the UCSC names as aliases +if (! $ucscNames) { + if ($isRefSeq) { + foreach my $ucscName (sort keys %ucscToRefSeq) { + if (defined($dupToSequence{$ucscToRefSeq{$ucscName}})) { # avoid duplicates + printf STDERR "# skipping duplicate name $ucscToRefSeq{$ucscName}\n"; + } else { + addAlias("ucsc", $ucscName, $ucscToRefSeq{$ucscName}); + } + } + } else { + foreach my $ucscName (sort keys %ucscToGenbank) { + if (defined($dupToSequence{$ucscToGenbank{$ucscName}})) { # avoid duplicates + printf STDERR "# skipping duplicate name $ucscToGenbank{$ucscName}\n"; + } else { + addAlias("ucsc", $ucscName, $ucscToGenbank{$ucscName}); + } + } + } +} + if ($sequenceCount != $nameCount) { printf STDERR "ERROR: do not find the same name count in sequence vs. names files\n"; printf STDERR "sequenceCount %d != %d names count - %d duplicates\n", $sequenceCount, $nameCount, $dupCount; exit 255; } # printf STDERR "# initial set of %d UCSC names\n", $sequenceCount; ### first set of names is the UCSC to NCBI sequence names foreach my $sequence (sort keys %sequenceSizes) { my $seqName = $sequence; + if (defined($dupToSequence{$seqName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $seqName\n"; + next; + } my $alias = $sequence; if ($ucscNames) { $alias = $ucscToNcbi{$seqName}; } else { $alias = $ncbiToUcsc{$seqName}; } if (!defined($alias)) { printf STDERR "ERROR: can not find alias name for '%s' sequence\n", $seqName; exit 255; } addAlias($ucscNames ? "ucsc" : $asmSource, $alias, $seqName); } # foreach my $sequence (sort keys %chrNames) { # printf "%s\t%s\n", $chrNames{$sequence}, $sequence; # } ### next set of names are the equivalents declared by NCBI ### if they exist my %chr2acc; # key is sequence name, value is NCBI chromosome name my $asmStructCount = `ls ../../download/${asmId}_assembly_structure/*/*/chr2acc 2> /dev/null | wc -l`; chomp $asmStructCount; if ( $asmStructCount > 0 ) { # printf STDERR "# second name set processing chr2acc files\n"; open (FH, "grep -h -v '^#' ../../download/${asmId}_assembly_structure/*/*/chr2acc|") or die "can not grep chr2acc files"; while (my $line = <FH>) { chomp $line; my ($alias, $seqName) = split('\t', $line); $alias =~ s/ /_/g; # some assemblies have spaces in chr names ... $alias =~ s/:/_/g; # one assembly had : in a chr name $chr2acc{$seqName} = $alias; if ($ucscNames) { $seqName = $ncbiToUcsc{$seqName}; } + if (defined($dupToSequence{$seqName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $seqName\n"; + } else { addAlias("ncbi", $alias, $seqName); } + } close (FH); } # foreach my $sequence (sort keys %chr2acc) { # printf "%s\t%s\n", $chr2acc{$sequence}, $sequence; # } my $dbgCount = 0; # printf STDERR "# third set processing assembly_report\n"; # column 1 is the 'assembly' name # column 5 is the GenBank-Accn, column 7 is the RefSeq-Accn open (FH, "grep -v '^#' ../../download/${asmId}_assembly_report.txt | cut -d\$'\t' -f1,5,7|") or die "can not grep assembly_report"; while (my $line = <FH>) { chomp $line; ++$dbgCount; my ($asmName, $gbkName, $refSeqName) = split('\s+', $line); + if (defined($dupToSequence{$asmName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $asmName\n"; + next; + } elsif (defined($dupToSequence{$gbkName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $gbkName\n"; + next; + } elsif (defined($dupToSequence{$refSeqName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $refSeqName\n"; + next; + } printf STDERR "# '%s'\t'%s'\t'%s'\n", $asmName, $gbkName, $refSeqName if ($dbgCount < 5); # next if ($refSeqName eq "na"); # may not be any RefSeq name # next if ($gbkName eq "na"); # may not be any GenBank name if ($refSeqName ne "na") { my $seqName = $refSeqName; if (! $isRefSeq) { $seqName = $gbkName; } if ($ucscNames) { $seqName = $ncbiToUcsc{$seqName}; } if (!defined($seqName)) { if (defined($aliasOut{"refseq"})) { if (defined($aliasOut{"refseq"}->{$refSeqName})) { $seqName = $aliasOut{"refseq"}->{$refSeqName}; } } } if (defined($seqName)) { if (defined($dupToSequence{$seqName})) { + if (defined($dupToSequence{$seqName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $dupToSequence{$seqName}\n"; + } else { addAlias("refseq", $refSeqName, $dupToSequence{$seqName}); addAlias("assembly", $asmName, $dupToSequence{$seqName}); + } + } else { + if (defined($dupToSequence{$seqName})) { # avoid duplicates + printf STDERR "# skipping duplicate name $seqName\n"; } else { addAlias("refseq", $refSeqName, $seqName); addAlias("assembly", $asmName, $seqName); } } + } } # if ($refSeqName ne "na") if ($gbkName ne "na") { my $seqName = $gbkName; if ($isRefSeq) { $seqName = $refSeqName; } if ($ucscNames) { $seqName = $ncbiToUcsc{$seqName}; } if (!defined($seqName)) { if (defined($aliasOut{"genbank"})) { if (defined($aliasOut{"genbank"}->{$gbkName})) { $seqName = $aliasOut{"genbank"}->{$gbkName}; } } } if (defined($seqName)) { if (defined($dupToSequence{$seqName})) { addAlias("genbank", $gbkName, $dupToSequence{$seqName}); addAlias("assembly", $asmName, $dupToSequence{$seqName}); } else { addAlias("genbank", $gbkName, $seqName); addAlias("assembly", $asmName, $seqName); } } } # if ($gbkName ne "na") } # done reading assembly_report.txt close (FH); showAlias($ucscNames ? "ucsc" : $asmSource);