2ffb313fbbddc5b678beb8a92da4d513c178268d
max
  Tue Apr 4 07:09:11 2023 -0700
got question from Jim R why long sequences do not work from IGV, so adding note to FAQ blat that HTTP POST is required for that, no redmine

diff --git src/hg/htdocs/FAQ/FAQblat.html src/hg/htdocs/FAQ/FAQblat.html
index 3e5a549..2c3eda2 100755
--- src/hg/htdocs/FAQ/FAQblat.html
+++ src/hg/htdocs/FAQ/FAQblat.html
@@ -475,25 +475,28 @@
 <a target="_blank"
 href="https://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html/index.cgi?chapter=cgencodes">
 NCBI website</a>. Additional details on mitochondria codon tables can be found on
 the <a target="_blank"
 href="https://www.wikiwand.com/en/DNA_and_RNA_codon_tables">Wikiwand website</a>.
 </p>
 
 <a name="blat14"></a>
 <h2>Querying BLAT programmatically using URLs</h2>
 <p>
 For programmatic access, BLAT supports URL queries which are returned as psl format in a JSON structure. 
 The URL requires three variables: the sequence to blat, the type of query and the database, as follows</p>
 <pre>
 https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=[seq]&amp;type=[type]&amp;db=[database]&amp;output=json</pre>
 <p>
+If the sequence is longer than 8kbp, the request must be sent via HTTP POST.
+</p>
+<p>
 Query types include DNA, protein, translated RNA and translated DNA:</p>
 <pre>
 <a href="https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=GACCTCGGCGTGGCCTAGCG&amp;type=DNA&amp;db=hg38&amp;output=json">https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=GACCTCGGCGTGGCCTAGCG&amp;type=DNA&amp;db=hg38&amp;output=json</a>
 <a href="https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNM&amp;type=protein&amp;db=hg38&amp;output=json">https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNM&amp;type=protein&amp;db=hg38&amp;output=json</a>
 <a href="https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=UUUCCCUUCCCCACUGUAGUGGGAGAGAAGGGAGUGGCCAUACCAUAUUUUUCUCGUGGGCCGUUGUAGUCAUAAGGCCUUCCUUUGCGGAAAAUUUUCAGGGUGGGAUA&amp;type=translated%20RNA&amp;db=hg38&amp;output=json">https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=UUUCCCUUCCCCACUGUAGUGGGAGAGAAGGGAGUGGCCAUACCAUAUUUUUCUCGUGGGCCGUUGUAGUCAUAAGGCCUUCCUUUGCGGAAAAUUUUCAGGGUGGGAUA&type=translated%20RNA&db=hg38&output=json</a>
 <a href="https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=TTTCCCTTCCCCACTGTAGTGGGAGAGAAGGGAGTGGCCATACCATATTTTTCTCGTGGGCCGTTGTAGTCATAAGGCCTTCCTTTGCGGAAAATTTTCAGGGTGGGATA&amp;type=translated%20DNA&amp;db=hg38&amp;output=json">https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=TTTCCCTTCCCCACTGTAGTGGGAGAGAAGGGAGTGGCCATACCATATTTTTCTCGTGGGCCGTTGTAGTCATAAGGCCTTCCTTTGCGGAAAATTTTCAGGGTGGGATA&amp;type=translated%20DNA&amp;db=hg38&amp;output=json</a>
 </pre>
 
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