4d7ad4d7fff4de41ae6ff3d1999df2cf640108db max Tue Aug 15 07:53:17 2023 -0700 docing primer3 formula that we use, refs #31950 diff --git src/hg/hgPcr/hgPcr.c src/hg/hgPcr/hgPcr.c index 77607de..e2d295c 100644 --- src/hg/hgPcr/hgPcr.c +++ src/hg/hgPcr/hgPcr.c @@ -702,31 +702,32 @@ } errCatchEnd(errCatch); if (errCatch->gotError) warn("%s", errCatch->message->string); errCatchFree(&errCatch); if (flipReverse) reverseComplement(rPrimer, strlen(rPrimer)); webNewSection("Primer Melting Temperatures"); printf(""); printf("Forward: %4.1f C %s
\n", oligoTm(fPrimer, 50.0, 50.0), fPrimer); printf("Reverse: %4.1f C %s
\n", oligoTm(rPrimer, 50.0, 50.0), rPrimer); printf("
"); printf("The temperature calculations are done assuming 50 mM salt and 50 nM annealing " "oligo concentration. The code to calculate the melting temp comes from " "" - "Primer3."); + "Primer3, the formula by Rychlik W, Spencer WJ and Rhoads RE NAR 1990, which can " + "be activated in Primer3 with PRIMER_TM_FORMULA=0."); webNewSection("Help"); printf("What is chr_alt & chr_fix?
"); printf("Replicating in-Silico PCR results on local machine"); return ok; } void dispatch() /* Look at input variables and figure out which page to show. */ { char *db, *organism; int maxSize = 4000; int minPerfect = 15; int minGood = 15; char *fPrimer = cartUsualString(cart, "wp_f", ""); char *rPrimer = cartUsualString(cart, "wp_r", "");