6f8a50068e6c1558dbbf286b9a573800c68b1fb1 braney Tue May 28 17:16:22 2024 -0700 ongoing work on pair browser diff --git src/hg/utils/buildPairAssembly/makePair src/hg/utils/buildPairAssembly/makePair index aa96a71..748685c 100755 --- src/hg/utils/buildPairAssembly/makePair +++ src/hg/utils/buildPairAssembly/makePair @@ -1,30 +1,30 @@ #!/bin/sh -ex pairRefDb=$1 pairRefSeq=$2 pairRefDb2bit=$3 pairQueryDb=$4 pairQuerySeq=$5 pairQueryDb2bit=$6 pairChain=$7 filterChain=$pairRefSeq.$pairQuerySeq.$pairChain chainFilter -t=$pairRefSeq -q=$pairQuerySeq $pairChain > $filterChain -buildPairAssembly $filterChain $pairRefSeq $pairRefDb2bit $pairQuerySeq $pairQueryDb2bit out.fa query.psl target.psl map.bed dup.bed miss.bed baseTarget.bed baseQuery.bed +buildPairAssembly $filterChain $pairRefSeq $pairRefDb2bit $pairQuerySeq $pairQueryDb2bit out.fa query.psl target.psl map.bed dup.bed miss.bed baseTarget.bed baseQuery.bed synBlocks.bed rm -rf hub mkdir hub faToTwoBit out.fa hub/buildPair.2bit twoBitInfo hub/buildPair.2bit hub/buildPair.sizes.txt bedToBigBed -type=bed9+4 map.bed hub/buildPair.sizes.txt hub/map.bb axtChain -psl -linearGap=medium query.psl hub/buildPair.2bit $pairQueryDb2bit query.chain hgLoadChain -noBin -test hg38 bigChain query.chain sed 's/\.000000//' chain.tab | awk 'BEGIN {OFS="\t"} {print $2, $4, $5, $11, 1000, $8, $3, $6, $7, $9, $10, $1}' > query.bigChain bedToBigBed -type=bed6+6 -as=$HOME/kent/src/hg/lib/bigChain.as -tab query.bigChain hub/buildPair.sizes.txt hub/queryChain.bb awk 'BEGIN {OFS="\t"} {print $1, $2, $3, $5, $4}' link.tab | sort -k1,1 -k2,2n > bigChain.bigLink bedToBigBed -type=bed4+1 -as=$HOME/kent/src/hg/lib/bigLink.as -tab bigChain.bigLink hub/buildPair.sizes.txt hub/queryChain.link.bb @@ -37,30 +37,33 @@ bedToBigBed -type=bed4+1 -as=$HOME/kent/src/hg/lib/bigLink.as -tab bigChain.bigLink hub/buildPair.sizes.txt hub/targetChain.link.bb chainSwap query.chain swap.query.chain chainSwap target.chain swap.target.chain awk '{print $1,$2,$3,$7}' dup.bed | liftOver -minMatch=0.001 stdin swap.query.chain tmp foo awk '{print $4,$5,$6,$7}' dup.bed | liftOver -minMatch=0.001 stdin swap.target.chain tmp2 foo sort tmp tmp2 > tmp3 bedToBigBed -type=bed4 -tab tmp3 hub/buildPair.sizes.txt hub/dups.bb bedToBigBed -type=bed4 miss.bed hub/buildPair.sizes.txt hub/mismatch.bb bedToBigBed -type=bed4+1 baseTarget.bed hub/buildPair.sizes.txt hub/baseTarget.bb bedToBigBed -type=bed4+1 baseQuery.bed hub/buildPair.sizes.txt hub/baseQuery.bb +sort -k1,1 -k2,2n synBlocks.bed > sorted.bed +bedToBigBed -type=bed4 sorted.bed hub/buildPair.sizes.txt hub/synBlocks.bb + genome=$pairQueryDb gcX="${genome:0:3}" d0="${genome:4:3}" d1="${genome:7:3}" d2="${genome:10:3}" tmp=`cd /usr/local/apache/htdocs/hubs; echo $gcX/$d0/$d1/$d2/$genome/bbi/*ens*.bb ` ensGeneBb=https://hgdownload.soe.ucsc.edu/hubs/$tmp tmp=`cd /usr/local/apache/htdocs/hubs; echo $gcX/$d0/$d1/$d2/$genome/bbi/*rmsk.bb ` rmskBb=https://hgdownload.soe.ucsc.edu/hubs/$tmp tmp=`cd /usr/local/apache/htdocs/hubs; echo $gcX/$d0/$d1/$d2/$genome/bbi/*simpleRepeat.bb ` simpleBb=https://hgdownload.soe.ucsc.edu/hubs/$tmp cat << _EOF_ > hub/groups.txt @@ -142,30 +145,38 @@ priority 0.1 type bigBaseView bigDataUrl baseQuery.bb shortLabel BaseView longLabel $pairQueryDb BaseView group query track baseTarget priority 0.1 type bigBaseView bigDataUrl baseTarget.bb shortLabel BaseView longLabel $pairRefDb BaseView group target +track synBlocks +priority 0.1 +type bigBed 4 +bigDataUrl synBlocks.bb +shortLabel synBlocks +longLabel Synteny Blocks +group map + track mismatch type bigBed 4 bigDataUrl mismatch.bb shortLabel Mismatches longLabel Mismatches between $pairRefDb and $pairQueryDb group map #track trmsk #type bigBed 9 #itemRgb on #shortLabel Rmsk #longLabel $pairRefDb Rmsk #bigDataUrl $rmskBb #quickLiftUrl targetChain.bb #group target