4f1877c2974ee37a984000e1add2c4c27da0de7b hiram Fri Oct 25 18:48:17 2024 -0700 the TMPDIR needs to be set locally on the para node not where the driver script is making the sub-script refs #34685 diff --git src/hg/utils/automation/asmHubRepeatMasker.sh src/hg/utils/automation/asmHubRepeatMasker.sh index 4678efe..c061941 100755 --- src/hg/utils/automation/asmHubRepeatMasker.sh +++ src/hg/utils/automation/asmHubRepeatMasker.sh @@ -1,152 +1,167 @@ #!/bin/bash # asmHubRepeatMasker.sh - process a specified *.fa.out.gz file into a set # of bigBed files for assembly hub display # # fail on any error: set -beEu -o pipefail # ensure sort functions properly despite kluster node environment export LC_COLLATE=C if [ $# -ne 3 ]; then printf "%s\n" "usage: asmHubRepeatMasker.sh <asmId> <pathTo/*fa.out.gz> <destinationDir/>" 1>&2 exit 255 fi +if [ -d "/data/tmp" ]; then + export TMPDIR="/data/tmp" +elif [ -d "/scratch/tmp" ]; then + export TMPDIR="/scratch/tmp" +else + tmpSz=`df --output=avail -k /tmp | tail -1` + shmSz=`df --output=avail -k /dev/shm | tail -1` + if [ "${shmSz}" -gt "${tmpSz}" ]; then + mkdir -p /dev/shm/tmp + chmod 777 /dev/shm/tmp + export TMPDIR="/dev/shm/tmp" + else + export TMPDIR="/tmp" + fi +fi export dateStamp=`date "+%FT%T %s"` export asmId=$1 export rmOutFile=$2 export destDir=$3 export chrSizes="" if [ -s "$destDir/$asmId.chrom.sizes" ]; then chrSizes="$destDir/$asmId.chrom.sizes" elif [ -s "../../$asmId.chrom.sizes" ]; then chrSizes="../../$asmId.chrom.sizes" fi # assume this file name pattern export faAlign=`echo "${rmOutFile}" | sed -e 's/sorted.fa.out/fa.align/; s/.gz//;'` export RepeatMaskerPath="/hive/data/staging/data/RepeatMasker221107" if [ -d "${destDir}" ]; then cd "${destDir}" # might already be gzipped if [ ! -s "${faAlign}" ]; then faAlign="${faAlign}.gz" fi # align file only exists when RM has been run locally, not for NCBI version # it is OK if it is missing, can do this anyway without it if [ -s "${faAlign}" ]; then printf "# using faAlign file: %s\n" "${faAlign}" 1>&2 printf "time $RepeatMaskerPath/util/rmToTrackHub.pl -sizes \"${chrSizes}\" -genome \"${asmId}\" -hubname \"${asmId}\" -out \"${rmOutFile}\" -align \"${faAlign}\"\n" 1>&2 time $RepeatMaskerPath/util/rmToTrackHub.pl -sizes "${chrSizes}" -genome "${asmId}" -hubname "${asmId}" -out "${rmOutFile}" -align "${faAlign}" # in place same file sort using the -o output option sort -k1,1 -k2,2n -o "${asmId}.fa.align.tsv" "${asmId}.fa.align.tsv" & else printf "# there is no faAlign file\n" 1>&2 printf "time $RepeatMaskerPath/util/rmToTrackHub.pl -sizes \"${chrSizes}\" -genome \"${asmId}\" -hubname \"${asmId}\" -out \"${rmOutFile}\"\n" 1>&2 time $RepeatMaskerPath/util/rmToTrackHub.pl -sizes "${chrSizes}" -genome "${asmId}" -hubname "${asmId}" -out "${rmOutFile}" fi sort -k1,1 -k2,2n -o "${asmId}.sorted.fa.join.tsv" "${asmId}.sorted.fa.join.tsv" & wait printf "bedToBigBed -tab -as=$HOME/kent/src/hg/lib/bigRmskBed.as -type=bed9+5 \"${asmId}.sorted.fa.join.tsv\" \"${chrSizes}\" \"${asmId}.rmsk.bb\" &\n" 1>&2 bedToBigBed -tab -as=$HOME/kent/src/hg/lib/bigRmskBed.as -type=bed9+5 \ "${asmId}.sorted.fa.join.tsv" "${chrSizes}" \ "${asmId}.rmsk.bb" & if [ -s "${asmId}.fa.align.tsv" ]; then printf "bedToBigBed -tab -as=$HOME/kent/src/hg/lib/bigRmskAlignBed.as -type=bed3+14 \"${asmId}.fa.align.tsv\" \"${chrSizes}\" \"${asmId}.rmsk.align.bb\" &\n" 1>&2 bedToBigBed -tab -as=$HOME/kent/src/hg/lib/bigRmskAlignBed.as \ -type=bed3+14 "${asmId}.fa.align.tsv" "${chrSizes}" \ "${asmId}.rmsk.align.bb" & fi wait if [ -s "${asmId}.fa.align.tsv" ]; then gzip "${asmId}.fa.align.tsv" & fi gzip "${asmId}.sorted.fa.join.tsv" & wait rm -fr classBed rmskClass ${asmId}.rmsk.tab bbi/*.rmsk.*.bb \ bbi/*.rmsk.*.bb mkdir classBed rmskClass rm -f ${asmId}.rm.out mkdir -p bbi dateStamp=`date "+%FT%T %s"` printf "# %s processing %s\n" "${dateStamp}" "${rmOutFile}" 1>&2 - export rmOutTmp=`mktemp -p /dev/shm rmskProcess.${asmId}.XXXXX` + export rmOutTmp=`mktemp -p "${TMPDIR}" rmskProcess.${asmId}.XXXXX` printf "%s\n" ' SW perc perc perc query position in query matching repeat position in repeat score div. del. ins. sequence begin end (left) repeat class/family begin end (left) ID ' > "${rmOutTmp}" zcat "${rmOutFile}" | headRest 3 stdin >> "${rmOutTmp}" hgLoadOut -verbose=2 -tabFile=${asmId}.rmsk.tab -table=rmsk -nosplit test "${rmOutTmp}" 2> rmsk.bad.records.txt dateStamp=`date "+%FT%T %s"` if [ -s ${asmId}.rmsk.tab ]; then printf "# %s splitting into categories %s\n" "${dateStamp}" "${asmId}.rmsk.tab" 1>&2 sort -k12,12 ${asmId}.rmsk.tab \ | splitFileByColumn -ending=tab -col=12 -tab stdin rmskClass for T in SINE LINE LTR DNA Simple Low_complexity Satellite do fileCount=`(ls rmskClass/${T}*.tab 2> /dev/null || true) | wc -l` if [ "$fileCount" -gt 0 ]; then dateStamp=`date "+%FT%T %s"` printf "# %s bedToBigBed on: rmskClass/%s*.tab " "${dateStamp}" "${T}" ls rmskClass/${T}*.tab | xargs echo $HOME/kent/src/hg/utils/automation/rmskBed6+10.pl rmskClass/${T}*.tab \ | sort -k1,1 -k2,2n > classBed/${asmId}.rmsk.${T}.bed bedToBigBed -tab -type=bed6+10 -as=$HOME/kent/src/hg/lib/rmskBed6+10.as \ classBed/${asmId}.rmsk.${T}.bed "${chrSizes}" \ bbi/${asmId}.rmsk.${T}.bb & fi done wait fileCount=`(ls rmskClass/*RNA.tab 2> /dev/null || true) | wc -l` if [ "$fileCount" -gt 0 ]; then dateStamp=`date "+%FT%T %s"` printf "# %s bedToBigBed on rmskClass/*RNA.tab: " "${dateStamp}" ls rmskClass/*RNA.tab | xargs echo $HOME/kent/src/hg/utils/automation/rmskBed6+10.pl rmskClass/*RNA.tab \ | sort -k1,1 -k2,2n > classBed/${asmId}.rmsk.RNA.bed bedToBigBed -tab -type=bed6+10 -as=$HOME/kent/src/hg/lib/rmskBed6+10.as \ classBed/${asmId}.rmsk.RNA.bed "${chrSizes}" \ bbi/${asmId}.rmsk.RNA.bb fi dateStamp=`date "+%FT%T %s"` printf "# %s checking ls rmskClass/*.tab | egrep -v \"/SIN|/LIN|/LT|/DN|/Simple|/Low_complexity|/Satellit|RNA.tab\"\n" "${dateStamp}" otherCount=`(ls rmskClass/*.tab 2> /dev/null || true) | (egrep -v "/SIN|/LIN|/LT|/DN|/Simple|/Low_complexity|/Satellit|RNA.tab" || true) | wc -l` if [ "${otherCount}" -gt 0 ]; then dateStamp=`date "+%FT%T %s"` printf "# %s bedToBigBed on: " "${dateStamp}" ls rmskClass/*.tab | egrep -v "/SIN|/LIN|/LT|/DN|/Simple|/Low_complexity|/Satellit|RNA.tab" | xargs echo | sed -e 's#rmskClass/##g;' $HOME/kent/src/hg/utils/automation/rmskBed6+10.pl `ls rmskClass/*.tab | egrep -v "/SIN|/LIN|/LT|/DN|/Simple|/Low_complexity|/Satellit|RNA.tab" | sed -e 's/^/"/; s/$/"/;'|xargs echo` \ | sort -k1,1 -k2,2n > classBed/${asmId}.rmsk.Other.bed bedToBigBed -tab -type=bed6+10 -as=$HOME/kent/src/hg/lib/rmskBed6+10.as \ classBed/${asmId}.rmsk.Other.bed "${chrSizes}" \ bbi/${asmId}.rmsk.Other.bb fi export bbiCount=`for F in bbi/*.rmsk.*.bb; do bigBedInfo $F | grep itemCount; done | awk '{print $NF}' | sed -e 's/,//g' | ave stdin | grep total | awk '{print $2}' | sed -e 's/.000000//'` export firstTabCount=`cat ${asmId}.rmsk.tab | wc -l` export splitTabCount=`cat rmskClass/*.tab | wc -l` if [ "$firstTabCount" -ne $bbiCount ]; then printf "%s.rmsk.tab count: %d, split class tab file count: %d, bbi class item count: %d\n" "${asmId}" $firstTabCount $splitTabCount $bbiCount 1>&2 printf "ERROR: did not account for all items in rmsk class bbi construction\n" 1>&2 exit 255 fi wc -l classBed/*.bed > ${asmId}.rmsk.class.profile.txt wc -l rmskClass/*.tab >> ${asmId}.rmsk.class.profile.txt rm -fr rmskClass classBed ${asmId}.rmsk.tab fi rm -f "${rmOutTmp}" else printf "ERROR: can not find destination directory '%s'\n" "${destDir}" 1>&2 exit 255 fi