8c2f7318d8d821de9b2a25750586a94ab5e8c1bb lrnassar Fri Nov 15 18:50:19 2024 -0800 Giving the UI link cronjob some love by fixing all the 301 redirects. These are the bulk of the items listed on the cron. No RM. diff --git src/hg/makeDb/trackDb/human/bamSLDenisova.html src/hg/makeDb/trackDb/human/bamSLDenisova.html index ef73330..a2f3ab9 100644 --- src/hg/makeDb/trackDb/human/bamSLDenisova.html +++ src/hg/makeDb/trackDb/human/bamSLDenisova.html @@ -24,31 +24,31 @@ <H2>Methods</H2> <P> Denisova sequence libraries were prepared by treating DNA extracted from a single phalanx bone with two enzymes: uracil-DNA-glycosylase, which removes uracil residues from DNA to leave abasic sites, and endonuclease VIII, which cuts DNA at the 59 and 39 sides of abasic sites. Subsequent incubation with T4 polynucleotide kinase and T4 DNA polymerase was used to generate phosphorylated blunt ends that are amenable to adaptor ligation. Because the great majority of uracil residues occur close to the ends of ancient DNA molecules, this procedure leads to only a moderate reduction in average length of the molecules in the library, but a several-fold reduction in uracil-derived nucleotide misincorporation. Reads were aligned to $organism sequence $date using the -<A HREF="http://bio-bwa.sourceforge.net/" +<A HREF="https://bio-bwa.sourceforge.net/" TARGET=_blank>Burrows-Wheeler Aligner.</A> </P> <P> <A HREF="ftp://hgdownload.soe.ucsc.edu/gbdb/hg18/denisova/">Download</A> the Denisova track data sets from the Genome Browser downloads server. </P> <H2>References</H2> <P> Briggs A.W., Stenzel U., Meyer M., Krause J., Kircher M., Pääbo S. <A HREF="https://academic.oup.com/nar/article/38/6/e87/3112544" TARGET=_blank> Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA</A>. <EM>Nucleic Acids Res.</EM> 2009 Dec 22:38(6) e87. </P>