This track shows potential enhancers whose activity was experimentally validated in transgenic mice. Most of these noncoding elements were selected for testing based on their extreme conservation in other vertebrates or epigenomic evidence (ChIP-Seq) of putative enhancer marks. More information -can be found on the VISTA Enhancer Browser +can be found on the VISTA Enhancer Browser page.
Items appearing in red (positive) indicate that a reproducible pattern was observed in the in vivo enhancer assay. Items appearing in blue (negative) indicate that NO reproducible pattern was observed in the in vivo enhancer assay. Note that this annotation refers only to the single developmental timepoint that was tested in this screen (e11.5) and does not exclude the possibility that this region is a reproducible enhancer active at earlier or later timepoints in development.
Excerpted from the Vista Enhancer Mouse Enhancer Screen Handbook and Methods page at the Lawrence Berkeley National Laboratory (LBNL) website:
Most enhancer candidate sequences are identified by extreme evolutionary sequence conservation or by ChIP-seq. Detailed information related to enhancer identification by extreme evolutionary conservation can be found in the following publications:
Detailed information related to enhancer identification by ChIP-seq can be found in the following publications:
See the Transgenic Mouse Assay section for experimental procedures that were used to perform the transgenic assays: Mouse Enhancer Screen Handbook and Methods
UCSC converted the Experimental Data for hg19 and mm9 into bigBed format using the bedToBigBed utility. The data for hg38 was lifted over from hg19. The data for mm10 and mm39 were lifted over from mm9.