1ee65ce95e96f67f08319b0310f494804453ecfc
jnavarr5
Mon Jun 9 15:06:40 2025 -0700
Adding a note that the Target option is only available for human and mouse assemblies, refs #35834
diff --git src/hg/hgPcr/hgPcr.c src/hg/hgPcr/hgPcr.c
index 3ee8c96c768..fd424d117f3 100644
--- src/hg/hgPcr/hgPcr.c
+++ src/hg/hgPcr/hgPcr.c
@@ -214,31 +214,31 @@
/* Print up help page */
{
printf(
"In-Silico PCR V%s searches a sequence database with a pair of\n"
"PCR primers, using the BLAT index for fast performance.\n"
"See an example\n"
"video\n"
"on our YouTube channel.
\n"
"This tool is not guaranteed to find absolutely all off-target locations,\n"
"it is optimized for targets with higher identities. For\n"
"use in primer design, especially in repetitive regions, consider additional validation with tools such as\n"
""
"primer blast.
\n"
"If you are looking for matches to RT-PCR primers, where primers often straddle intron-exon boundaries, change the Target option and select "
-"a gene transcript set.
\n"
+"a gene transcript set. This feature is only available for human and mouse assemblies.
\n"
"Configuration Options
\n"
"Genome and Assembly - The sequence database to search.
\n"
"Target - If available, choose to query transcribed sequences.
\n"
"Forward Primer - Must be at least 15 bases in length.
\n"
"Reverse Primer - On the opposite strand from the forward primer. Minimum length of 15 bases.
\n"
"Max Product Size - Maximum size of amplified region.
\n"
"Min Perfect Match - Number of bases that match exactly on 3' end of primers. Minimum match size is 15.
\n"
"Min Good Match - Number of bases on 3' end of primers where at least 2 out of 3 bases match.
\n"
"Flip Reverse Primer - Invert the sequence order of the reverse primer and complement it.
\n"
"Append to existing PCR result - Add this PCR result list to the currently existing track of PCR results.
\n"
"\n"
"Output
\n"
"When successful, the search returns a sequence output file in fasta format \n"
"containing all sequence in the database that lie between and include the \n"
"primer pair. The fasta header describes the region in the database\n"