1eb778792659124aa071876e2c5541bc8e3d6b3d max Tue Nov 4 01:54:21 2025 -0800 adding a link to more updated ancient dna tracks, refs #36592 diff --git src/hg/makeDb/trackDb/human/bamSLDenisova.html src/hg/makeDb/trackDb/human/bamSLDenisova.html index a2f3ab9c52e..7497eefd6ba 100644 --- src/hg/makeDb/trackDb/human/bamSLDenisova.html +++ src/hg/makeDb/trackDb/human/bamSLDenisova.html @@ -4,32 +4,41 @@ <IMG SRC="../images/denisovaCave.jpg" WIDTH=426 HEIGHT=640 ALT="Denisova cave"> </TD></TR> <TR><TD ALIGN=RIGHT> <FONT SIZE=-1>Denisova cave entrance in the Altai Mountains of Siberia, Russia where the bones were found from which DNA was sequenced </FONT> <FONT SIZE=-2> (Copyright (C) 2010, Johannes Krause) </FONT> </TD></TR> </TABLE> <H2>Description</H2> <P> -The Denisova track shows Denisova sequence reads mapped to the -$organism genome. The Denisova sequence was generated from a phalanx bone +This track shows Denisova sequence reads mapped to the +human genome, but to the old hg18 assembly from 2006. We provide updated annotation +tracks with the called variants on more recent assemblies: +<a href="hgTrackUi?g=dhcVcfDenisovaPinky&hgTracksConfigPage=configure">Denisova variants on hg19</a>, +and <a href="hgTrackUi?g=ancient&hgTracksConfigPage=configure">Neanderthal and Denisova variants on hg38</a>. +There is no actual "Neanderthal" or "Denisova" genome assembly, the short fragments obtained from ancient +samples can be aligned to the human genome and analyses are based on short variants. +</P> + +<P> +The Denisova sequence was generated from a phalanx bone excavated from Denisova Cave in the Altai Mountains in southern Siberia. </P> <H2>Methods</H2> <P> Denisova sequence libraries were prepared by treating DNA extracted from a single phalanx bone with two enzymes: uracil-DNA-glycosylase, which removes uracil residues from DNA to leave abasic sites, and endonuclease VIII, which cuts DNA at the 59 and 39 sides of abasic sites. Subsequent incubation with T4 polynucleotide kinase and T4 DNA polymerase was used to generate phosphorylated blunt ends that are amenable to adaptor ligation. Because the great majority of uracil residues occur close to the ends of ancient DNA molecules, this procedure leads to only a moderate reduction in average length of the