7594507ca126d5242346787e42e13c52ea7709b1 max Fri Apr 17 08:40:31 2026 -0700 Add lrSv supertrack: long-read structural variants from 9 studies (hg38). #Preview2 week - bugs introduced now will need a build patch to fix Sub-tracks (all bigBed 9+): han945Sv - 945 Han Chinese, ONT (Gong 2025, PMID 39929826) lrSv1kgOnt - 1019 1000 Genomes, ONT, SVAN-annotated (Schloissnig 2025, PMID 40702182; lifted from hs1) tommoJpSv - 333 Japanese (111 trios), ONT (Otsuki 2022, PMID 36127505) aou1kSv - 1027 All of Us, PacBio HiFi (Garimella 2025, PMID 41256123) ga4kSv - 502 GA4K pediatric rare disease, PacBio HiFi (Cohen 2022, PMID 35305867) decodeSv - 3622 Icelanders, ONT (Beyter 2021, PMID 33972781) hgsvc3Sv - 65 HGSVC3 diverse haplotype-resolved assemblies, HiFi+ONT (Logsdon 2025, PMID 40702183; merges insdel+inv tables) kwanhoSv - 100 post-mortem brains (PD/ILBD/HC), PacBio HiFi (Kim 2026, PMID 41929179) chirmade101Sv - 101 long-read WGS GWAS SVatalog cohort (Chirmade 2026, PMID 41203876) Includes per-track conversion scripts and autoSql under scripts/lrSv/, the supertrack summary table in lrSv.html, and a consolidated makeDoc at doc/hg38/lrSv.txt. refs #36258 Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> diff --git src/hg/makeDb/trackDb/human/aou1kSv.html src/hg/makeDb/trackDb/human/aou1kSv.html new file mode 100644 index 00000000000..bafede33425 --- /dev/null +++ src/hg/makeDb/trackDb/human/aou1kSv.html @@ -0,0 +1,95 @@ +<h2>Description</h2> +<p> +This track shows structural variants (SVs) identified by PacBio HiFi long-read +sequencing of 1,027 individuals from the All of Us (AoU) Research Program. +Participants self-identified as Black or African American and were sequenced +to ~8x coverage. The dataset contains 541,049 SVs (444,524 insertions and +96,525 deletions) on autosomes. +</p> +<p> +SVs are annotated with population-specific allele frequencies across five +ancestry groups (African, Admixed American, East Asian, European, South Asian), +gene intersections from curated disease gene lists (OMIM, ACMG, cancer genes), +regulatory element overlaps, and associations with eQTLs, GWAS loci, and +clinical phenotypes from the AoU electronic health records. +</p> + +<h2>Display Conventions and Configuration</h2> +<p> +Items are colored by SV type: +<ul> +<li><span style="color: rgb(200,0,0);">Deletions (DEL)</span> - red</li> +<li><span style="color: rgb(0,0,200);">Insertions (INS)</span> - blue</li> +</ul> +</p> +<p> +Filters are available for SV type, SV length, and population-specific allele +frequencies. For insertions, the item is placed at the insertion site with a +width of 1 bp; for deletions, the item spans the deleted region. +</p> +<p> +The detail page shows the following annotations when available: +<ul> +<li><b>Population Allele Frequencies</b>: separate frequencies for AFR, AMR, +EAS, EUR, and SAS ancestry groups</li> +<li><b>Fst</b>: fixation index between African and non-African populations</li> +<li><b>Gene Intersections</b>: overlapping OMIM, disease, cancer, and ACMG +genes with constraint scores (pLI and LOEUF)</li> +<li><b>Regulatory Elements</b>: intersected regulatory elements (e.g. enhancer, +promoter)</li> +<li><b>Other LR Datasets</b>: whether the SV was also detected in HPRC, HGSVC, +or 1KG-ONT long-read datasets</li> +<li><b>eQTLs</b>: expression QTL associations with q-values</li> +<li><b>GWAS Associations</b>: overlapping GWAS loci with trait, gene, rsID, +and LD information</li> +<li><b>SV-Trait Associations</b>: associations with clinical phenotypes from +AoU electronic health records, including odds ratios and confidence +intervals</li> +</ul> +</p> + +<h2>Methods</h2> +<p> +PacBio HiFi long-read sequencing was performed on 1,027 AoU participants +self-identifying as Black or African American, at a median coverage of ~8x. +SV calling was performed using a cohort-level pipeline, producing calls for +insertions and deletions. Allele frequencies were computed separately for +five ancestry groups. SVs were annotated with gene intersections from OMIM, +disease gene panels, cancer gene lists, and ACMG actionable genes, along +with regulatory element overlaps and segmental duplication associations. +</p> +<p> +A scalable imputation workflow was developed to impute over 750,000 SVs into +existing short-read whole-genome sequencing datasets. SV-trait associations +were tested in 848 AoU participants with matched electronic health records, +identifying 291 significant associations across 226 conditions. +</p> + +<h2>Data Access</h2> +<p> +This track was built from supplementary data (media-2) of the AoU long-read +sequencing preprint. Access to the full AoU dataset requires registration +through the <a href="https://www.researchallofus.org/" target="_blank">All of +Us Research Hub</a>. +</p> + +<h2>Credits</h2> +<p> +Thanks to Garimella et al. and the All of Us Research Program for making their +structural variant annotations publicly available. +</p> + +<h2>References</h2> + + + +<p> +Garimella KV, Li Q, Wertz J, Lee SK, Cunial F, Huang Y, Mostovoy Y, Lorig-Roach R, English A, Su H +<em>et al</em>. +<a href="https://doi.org/10.1101/2025.10.02.25336942" target="_blank"> +Population-scale Long-read Sequencing in the All of Us Research Program</a>. +<em>medRxiv</em>. 2025 Oct 5;. +PMID: <a href="https://www.ncbi.nlm.nih.gov/pubmed/41256123" target="_blank">41256123</a>; PMC: <a +href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622093/" target="_blank">PMC12622093</a> +</p> +