7594507ca126d5242346787e42e13c52ea7709b1 max Fri Apr 17 08:40:31 2026 -0700 Add lrSv supertrack: long-read structural variants from 9 studies (hg38). #Preview2 week - bugs introduced now will need a build patch to fix Sub-tracks (all bigBed 9+): han945Sv - 945 Han Chinese, ONT (Gong 2025, PMID 39929826) lrSv1kgOnt - 1019 1000 Genomes, ONT, SVAN-annotated (Schloissnig 2025, PMID 40702182; lifted from hs1) tommoJpSv - 333 Japanese (111 trios), ONT (Otsuki 2022, PMID 36127505) aou1kSv - 1027 All of Us, PacBio HiFi (Garimella 2025, PMID 41256123) ga4kSv - 502 GA4K pediatric rare disease, PacBio HiFi (Cohen 2022, PMID 35305867) decodeSv - 3622 Icelanders, ONT (Beyter 2021, PMID 33972781) hgsvc3Sv - 65 HGSVC3 diverse haplotype-resolved assemblies, HiFi+ONT (Logsdon 2025, PMID 40702183; merges insdel+inv tables) kwanhoSv - 100 post-mortem brains (PD/ILBD/HC), PacBio HiFi (Kim 2026, PMID 41929179) chirmade101Sv - 101 long-read WGS GWAS SVatalog cohort (Chirmade 2026, PMID 41203876) Includes per-track conversion scripts and autoSql under scripts/lrSv/, the supertrack summary table in lrSv.html, and a consolidated makeDoc at doc/hg38/lrSv.txt. refs #36258 Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> diff --git src/hg/makeDb/trackDb/human/kwanhoSv.html src/hg/makeDb/trackDb/human/kwanhoSv.html new file mode 100644 index 00000000000..d888c3a6f52 --- /dev/null +++ src/hg/makeDb/trackDb/human/kwanhoSv.html @@ -0,0 +1,106 @@ +<h2>Description</h2> +<p> +This track shows structural variants (SVs) identified by PacBio HiFi long-read +whole-genome sequencing of 100 post-mortem human brain samples, split across +three diagnostic groups: Parkinson's disease (PD), incidental Lewy body +disease (ILBD) and healthy controls (HC). The high-confidence catalog +contains 74,552 SVs: 34,056 insertions, 29,545 deletions, 9,707 duplications +and 1,244 inversions. +</p> +<p> +The dataset accompanies Kim et al. (2026), which combines the long-read SV +catalog with single-nucleus RNA-seq from the same donors to identify SVs +associated with cell-type-specific gene expression, including variants +near genes nominated as causal targets of PD GWAS loci. +</p> + +<h2>Display Conventions and Configuration</h2> +<p> +Items are colored by SV type: +<ul> +<li><span style="color: rgb(200,0,0);">Deletions (DEL)</span> - red</li> +<li><span style="color: rgb(0,0,200);">Insertions (INS)</span> - blue</li> +<li><span style="color: rgb(0,160,0);">Duplications (DUP)</span> - green</li> +<li><span style="color: rgb(230,140,0);">Inversions (INV)</span> - orange</li> +</ul> +</p> +<p> +Insertions are placed at the insertion site with a width of 1 bp; deletions, +duplications and inversions span the affected reference interval. Filters are +available for SV type, SV length, variant quality and allele frequencies in +each of the three cohorts (PD, HC, ILBD), as well as the case-minus-control +carrier-rate differential. +</p> +<p> +The detail page shows, for each variant: +<ul> +<li><b>Cohort support vector</b>: three-bit flag (PD/ILBD/HC) indicating +which cohorts include at least one carrier.</li> +<li><b>Carrier rates</b>: fraction of cases (PD+ILBD) and controls (HC) +carrying the variant, and the case-minus-control differential.</li> +<li><b>Per-cohort AF / AC / AN</b>: alternate allele frequency, alternate +allele count, and total called alleles in PD, HC and ILBD samples.</li> +<li><b>Carrier lists</b>: sample IDs carrying the variant in each cohort.</li> +<li><b>Nearby SNP context</b>: number of SNPs nearby and the number in +linkage disequilibrium with the SV (from the paper's LD analyses).</li> +<li><b>Read support</b>: average mapping quality and average supporting +reads per sample at the variant site.</li> +</ul> +</p> + +<h2>Methods</h2> +<p> +Long-read whole-genome sequencing was performed on 100 post-mortem brain +samples (35 PD, 31 ILBD, 34 HC) with PacBio HiFi chemistry. Per-sample SV +calls from multiple callers were merged into a joint callset; the +high-confidence filtered catalog released in Supplementary Table 13 +(<tt>media-13.txt</tt>) of the Kim et al. 2026 preprint is used directly +here. Per-cohort allele frequencies, Hardy-Weinberg statistics and case / +control carrier rates are reported in the source table; the track exposes +the allele counts and the case-control differential as filterable fields. +The paper also integrates single-nucleus RNA-seq from two brain regions +of the same donors to test SV-expression associations in specific cell +types, but that layer is not shown in this track. +</p> + +<h2>Data Access</h2> +<p> +The data can be explored interactively in table format with the +<a href="../cgi-bin/hgTables">Table Browser</a> or the +<a href="../cgi-bin/hgIntegrator">Data Integrator</a>, and accessed +programmatically through our <a href="https://api.genome.ucsc.edu">API</a>, +track=<i>kwanhoSv</i>. +</p> +<p> +The bigBed is available from +<a href="http://hgdownload.soe.ucsc.edu/gbdb/hg38/lrSv/" target="_blank">our +download server</a> as <tt>kwanho.bb</tt>. Example: +<tt>bigBedToBed http://hgdownload.soe.ucsc.edu/gbdb/hg38/lrSv/kwanho.bb -chrom=chr21 -start=0 -end=100000000 stdout</tt>. +</p> +<p> +The full supplementary data for the paper (including <tt>media-13.txt</tt>) +is available from the Kim et al. 2026 preprint. +</p> + +<h2>Credits</h2> +<p> +Thanks to Kim, Levin and colleagues at the Aligning Science Across +Parkinson's (ASAP) Collaborative Research Network, the Broad Institute, +Yale School of Medicine, Banner Sun Health Research Institute and their +collaborators for releasing this dataset. +</p> + +<h2>References</h2> + + +<p> +Kim K, Lin Z, Simmons SK, Parker J, Kearney M, Liao Z, Haywood N, Zhang J, Cline MP, Tuncali I +<em>et al</em>. +<a href="https://doi.org/10.64898/2026.03.20.713192" target="_blank"> +Integrating Long-Read Structural Variant Analysis with single-nucleus RNA-seq to Elucidate Gene +Expression Effects in Disease</a>. +<em>bioRxiv</em>. 2026 Mar 23;. +PMID: <a href="https://www.ncbi.nlm.nih.gov/pubmed/41929179" target="_blank">41929179</a>; PMC: <a +href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041997/" target="_blank">PMC13041997</a> +</p> +