7594507ca126d5242346787e42e13c52ea7709b1 max Fri Apr 17 08:40:31 2026 -0700 Add lrSv supertrack: long-read structural variants from 9 studies (hg38). #Preview2 week - bugs introduced now will need a build patch to fix Sub-tracks (all bigBed 9+): han945Sv - 945 Han Chinese, ONT (Gong 2025, PMID 39929826) lrSv1kgOnt - 1019 1000 Genomes, ONT, SVAN-annotated (Schloissnig 2025, PMID 40702182; lifted from hs1) tommoJpSv - 333 Japanese (111 trios), ONT (Otsuki 2022, PMID 36127505) aou1kSv - 1027 All of Us, PacBio HiFi (Garimella 2025, PMID 41256123) ga4kSv - 502 GA4K pediatric rare disease, PacBio HiFi (Cohen 2022, PMID 35305867) decodeSv - 3622 Icelanders, ONT (Beyter 2021, PMID 33972781) hgsvc3Sv - 65 HGSVC3 diverse haplotype-resolved assemblies, HiFi+ONT (Logsdon 2025, PMID 40702183; merges insdel+inv tables) kwanhoSv - 100 post-mortem brains (PD/ILBD/HC), PacBio HiFi (Kim 2026, PMID 41929179) chirmade101Sv - 101 long-read WGS GWAS SVatalog cohort (Chirmade 2026, PMID 41203876) Includes per-track conversion scripts and autoSql under scripts/lrSv/, the supertrack summary table in lrSv.html, and a consolidated makeDoc at doc/hg38/lrSv.txt. refs #36258 Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> diff --git src/hg/makeDb/trackDb/human/tommoJpSv.html src/hg/makeDb/trackDb/human/tommoJpSv.html new file mode 100644 index 00000000000..bf20054adf7 --- /dev/null +++ src/hg/makeDb/trackDb/human/tommoJpSv.html @@ -0,0 +1,81 @@ +<h2>Description</h2> +<p> +This track shows structural variants (SVs) identified by Oxford Nanopore long-read +sequencing of 333 Japanese individuals from the Tohoku Medical Megabank (ToMMo) +project. The 333 individuals form 111 parent-offspring trios, enabling +Mendelian consistency checks on the SV calls. Activated T lymphocytes were used +as a source of high-molecular-weight DNA for nanopore sequencing at a median +coverage of 22.2x with an N50 read length of 25.8 kb. +</p> +<p> +The dataset contains 74,201 SVs (37,981 deletions and 36,220 insertions), +merged across individuals using SURVIVOR v1.0.6. Over 95% of the SVs are +concordant with Mendelian inheritance in the trio families. +</p> + +<h2>Display Conventions and Configuration</h2> +<p> +Items are colored by SV type: +<ul> +<li><span style="color: rgb(200,0,0);">Deletions (DEL)</span> - red</li> +<li><span style="color: rgb(0,0,200);">Insertions (INS)</span> - blue</li> +</ul> +</p> +<p> +Filters are available for SV type, SV length, and allele frequency. +For insertions, the item is placed at the insertion site with a width of 1 bp; +for deletions, the item spans the deleted region. +</p> +<p> +The detail page for each item shows: +<ul> +<li><b>Allele Frequency</b>: fraction of alleles carrying this variant +(based on 444 alleles from 222 unrelated parents)</li> +<li><b>Allele Count / Allele Number</b>: number of variant alleles and +total alleles genotyped</li> +<li><b>Mendelian Error Rate</b>: fraction of trio families showing +inheritance errors for this variant</li> +<li><b>Families with Errors / Families Genotyped</b>: number of families +with Mendelian errors and total families with complete genotype calls</li> +</ul> +</p> + +<h2>Methods</h2> +<p> +Oxford Nanopore sequencing was performed on genomic DNA extracted from activated +T lymphocytes of 333 individuals (111 trios) from the Tohoku Medical Megabank +(ToMMo) cohort. SV calling was performed with Sniffles on each sample, and +calls were merged across individuals with SURVIVOR v1.0.6 using a maximum +distance of 1 kbp. Allele frequencies were computed from 222 unrelated parents +(excluding offspring to avoid double-counting). Mendelian error rates were +calculated by checking transmission consistency within each trio family. +</p> + +<h2>Data Access</h2> +<p> +Source data is available from the +<a href="https://jmorp.megabank.tohoku.ac.jp/datasets/tommo-jsv1-20211208-af" + target="_blank">jMorp data portal</a> (ToMMo Japanese Multi Omics Reference Panel). +</p> + +<h2>Credits</h2> +<p> +Thanks to the Tohoku Medical Megabank Organization for making their structural +variant calls publicly available through the jMorp data portal. +</p> + +<h2>References</h2> + + + +<p> +Otsuki A, Okamura Y, Ishida N, Tadaka S, Takayama J, Kumada K, Kawashima J, Taguchi K, Minegishi N, +Kuriyama S <em>et al</em>. +<a href="https://doi.org/10.1038/s42003-022-03953-1" target="_blank"> +Construction of a trio-based structural variation panel utilizing activated T lymphocytes and long- +read sequencing technology</a>. +<em>Commun Biol</em>. 2022 Sep 20;5(1):991. +PMID: <a href="https://www.ncbi.nlm.nih.gov/pubmed/36127505" target="_blank">36127505</a>; PMC: <a +href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489684/" target="_blank">PMC9489684</a> +</p> +