089c09e98db52e92da694ab1a0c8891c1a7fd3e8 gperez2 Thu Apr 2 15:50:07 2026 -0700 Fix broken external links in trackDb HTML docs found by uiLinks cron (2026-03-23 run). Updated 261 trackDb HTML and .ra files: - Fixed 404s: replaced dead URLs (sequenceontology.org wiki, compgen.cshl.edu PDFs, chori.org, PolyPhen v1, uswest.ensembl.org, RIKEN GSC) with current locations; removed links for defunct sites (snpdata.cshl.edu, zfrhmaps, renlab encode3 download, oia.gachon.ac.kr, phylohmm.pdf across 92 conservation track docs). - Fixed 301s: updated http→https (encodeproject.org, flybase.org, gene-regulation.com, drive5.com/muscle, microbesonline.org/fasttree, brain-map.org); updated moved domains (blackwell-synergy→wiley, genome.cshlp.org path fix, sanger.ac.uk path fix); removed hijacked domains (seqll.com, orfeomecollaboration.org, farnhamlab.com). - Fixed 302s: updated http→https (ensembl.org, affymetrix.com, asntech.org, cdna.eva.mpg.de, arcseqhub.com); updated moved domains (dfam.janelia.org→dfam.org, hmmer.janelia.org→hmmer.org, immuneepitope.org→iedb.org, ensembl variation path). No RM. diff --git src/hg/makeDb/trackDb/human/encodeUvaDnaRepTr50.html src/hg/makeDb/trackDb/human/encodeUvaDnaRepTr50.html index e9c6a48525a..328b9c7961a 100644 --- src/hg/makeDb/trackDb/human/encodeUvaDnaRepTr50.html +++ src/hg/makeDb/trackDb/human/encodeUvaDnaRepTr50.html @@ -1,64 +1,64 @@ <H2>Description</H2> <P> This annotation shows smoothed replication timing for DNA synthesis as the time of 50% replication (TR<SUB>50</SUB>).</P> <H2>Display Conventions and Configuration</H2> <P> This annotation follows the display conventions for composite tracks. The subtracks within this annotation may be configured in a variety of ways to highlight different aspects of the displayed data. The graphical configuration options are shown at the top of the track description page, followed by a list of subtracks. To display only selected subtracks, uncheck the boxes next to the tracks you wish to hide. For more information about the graphical configuration options, click the <A HREF="../goldenPath/help/hgWiggleTrackHelp.html" TARGET=_blank>Graph configuration help</A> link.</P> <H2>Methods</H2> <P> The experimental strategy adopted to map this profile involved isolation of replication products from HeLa cells synchronized at the G1-S boundary by thymidine-aphidicolin double block. Cells released from the block were labeled with BrdU at every two-hour interval of the 10 hours of S-phase and DNA was isolated from them. The heavy-light (H/L) DNA representing the pool of DNA replicated during each two-hour labeling period was separated from the unlabeled DNA by double cesium chloride density gradient centrifugation. The purified H/L DNA was then hybridized to a high-density genome-tiling Affymetrix array comprised of all unique probes within the ENCODE regions.</P> <P> The time of replication of 50% (TR<SUB>50</SUB>) of each microarray probe was calculated by accumulating the sum over the five time points and linearly interpolating the time when 50% was reached. Each probe was also classified as temporally specific or non-specific based on whether at least 50% of the accumulated signal appeared in a single time point or not.</P> <P> The TR<SUB>50</SUB> data for all specific probes were then lowess-smoothed within a 60 kb window to provide the profile displayed in the annotation.</P> <H2>Verification</H2> <P> The replication experiments were completed for two biological sets in the HeLa adherent cell line.</P> <H2>Credits</H2> <P> Data generation and analysis for this track were performed by the -DNA replication group in the <A HREF="http://genome.bioch.virginia.edu/" +DNA replication group in the <A HREF="https://app05.bioinformatics.virginia.edu/" TARGET=_blank>Dutta Lab</A> at the University of Virginia: Neerja Karnani, Christopher Taylor, Hakkyun Kim, Louis Lim, Ankit Malhotra, Gabe Robins and Anindya Dutta.</P> <P> Neerja Karnani and Christopher Taylor prepared the data for presentation in the UCSC Genome Browser. </P> <H2>References</H2> <P> Jeon, Y., Bekiranov, S., Karnani, N., Kapranov, P., Ghosh, S., MacAlpine, D., Lee, C., Hwang, D.S., Gingeras, T.R. and Dutta, A. <A HREF="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15845769&query_hl=1" TARGET=_blank>Temporal profile of replication of human chromosomes</A>. <em>Proc Natl Acad Sci U S A</em> <B>102</B>(18), 6419-24 (2005).